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Effect of compound Yindan decoction on alpha-naphthylisothiocyanate-Induced acute intrahepatic cholestasis in rats

Journal of Traditional Chinese Medicine 2019年第3期39卷 315-323页

作者： Sun Fengxia Li Xiaoling Xu Chunjun Sui Jingli Li Pan Wu Jingjing Department of Infections Beijing Hospital of Traditional Chinese Medicine Affiliated to the Capital Medical University No.23 Back Road of the Art Gallery Dongcheng District Beijing 100010 China Infections Department Beijing Hospital of Traditional Chinese Medicine Shunyi Branch Beijing 101300China

OBJECTIVE:To investigate the therapeutic mechanism of compound Yindan decoction (CYD) in a rat model of acute intrahepatic cholestatic (AIC).METHODS:A total of 108 adult male rats were randomly divided into control (n =18) and AIC groups (n =90).AIC was induced in rats using alpha-naphthylisothiocyanate (ANIT)(75 mg/kg,10 mL/kg in corn oil,p.o.).Then,90 AIC rats were randomly divided into five groups:a control group (n =18),a CYD high dose group (n =18),a CYD middle dose group (n =18),a CYD low dose group (n =18),and a ursodeoxycholicacid (UDCA) group (n =18).According to sampling time,each group was subdivided into three subgroups:24 h (n =6),48 h (n =6),and 72 h groups (n =6).The CYD-high,-middle and-low groups were orally administered 24.48,12.24,and 6.12 ***-1.d-1 modified CYD,respectively,while the model group was given 20 mL/kg of body weight of distilled water once a *** UDCA group was given 67.5 ***-1.d-1 UDCA once a *** assay was used to detect the activity of alanine aminotransferase (ALT),aspartate aminotransferase (AST),alkaline phosphatase (ALP),gamma-glutamyl transpeptidase (GGT) and the levels of total bilirubin (TBil) and indirectbiliruin (DBil) in *** transcription quantitative polymerase chain reaction (qRT-PCR),Western blot analysis,and immunohistochemistry were used to detect multidrug resistance-associated protein 2 (MRP2) *** vitro,HepG2 hepatocellular carcinoma cells were treated with CYD medicated serum at a concentration of 15 mol/***2 and retinoid X receptor alpha (RXRα) expression was analyzed by qRT-PCR and Western ***:Serum levels of ALT,AST,GGT,ALP,TBil,and DBil were significantly reduced in the CYD and positive drug groups compared with the control group (P < 0.05 and P < 0.01,respectively).Pathological changes in rat liver tissues at 72 h in the CYD-high and-medium dose groups and positive drug group were not significant compared with the control *** and UDCA treatment ameli

关键词： cholestasis,intrahepatic Multidrug resistance-associated proteins Retinoid X receptor alpha Compound Yindan decoction

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Clinical Management Guidelines for intrahepatic cholestasis of Pregnancy

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Maternal-Fetal Medicine 2024年第1期6卷 13-22页

作者： Obstetrics Group of the Gynecology and Obstetrics Branch of Chinese Medical Association Perinatal Medicine Branch of Chinese Medical Association Xinyang Yu Huixia Yang Hongbo Qi 不详 Medical University Chongqing 400016China Department of Obstetrics and Gynecology Peking University First HospitalBeijing 100034China Department of Obstetrics and Gynecology Women and Children's Hospital of Chongqing Medical UniversityChongqing 401147China

intrahepatic cholestasis of pregnancy(ICP)is a significant gestational complication in late pregnancy,potentially leading to severe perinatal complications such as intrauterine fetal demise and preterm *** Obstetrics Group of the Gynecology and Obstetrics Branch and the Perinatal Medicine Branch of the Chinese Medical Association organized a panel of domestic experts to deliberate and propose recommendations based on domestic and international guidelines,recent evidence-based medical evidence about key clinical issues including risk factors,clinical manifestations,perinatal outcomes,diagnosis,severity grading,maternal and fetal monitoring,treatment methods,timing,and methods of pregnancy termination,and postpartum follow-up for ICP,with the aim to guide its clinical diagnosis,treatment,and management.

关键词： cholestasis,intrahepatic Bile Acids Stillbirth Preterm Birth Diagnostic and Therapeutic Guidelines(Theme)

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Impaired Compensatory Vasodilatory Effect Mediated by Wolfram Syndrome 1 and Corticotropin-Releasing Hormone Family Peptides in 17α-Ethynylestradiol-Induced intrahepatic cholestasis Pregnant Rats When Under Additional Acute Hypoxia Stress

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Maternal-Fetal Medicine 2022年第1期4卷 7-16页

作者： Tingting Xu Daijuan Chen Xixi Deng Yongchi Zhan Fan Zhou Xiaodong Wang Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversitySichuan 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationSichuan 610041China

Objective:To investigate the possible regulatory mechanism of corticotropin-releasing hormone(CRH),urocortin(UCN),and Wolfram syndrome 1(WFS1)in 17α-ethynylestradiol(EE)-induced intrahepatic cholestasis pregnant rats and its ischemia reperfusion(IR)***:Pregnant rats(n=60)were randomly divided into four experimental groups by random number table(Control,EE,IR,and EE-IR groups),and were studied on the 17^(th),19th,and 21st gestational days(GD)(n=5 in each group at the indicated time).Growth and development indicators of fetal rats among these four groups were ***-linked immunosorbent assay was employed to detect CRH,UCN,and WFS1 levels in maternal *** blotting and real-time polymerase chain reaction were used to quantify placental protein and placental mRNA levels of CRH,UCN,and *** analysis of variance and least significant difference test were used to establish the group and individual ***:A significant difference was found in placenta weight(F=8.10,P0.05),and IR(0.46±0.15vs.0.22±0.15,P>0.05)groups at 19th GD,indicating that endoplasmic reticulum stress may be ***,the expression of CRH(0.42±0.05vs.0.58±0.12,P0.05),and WFS1(0.57±0.07vs.0.74±0.12,P>0.05)protein in the EE-IR group was subsided compared to the IR group at 17^(th) ***:Fetal rat growth restriction was found in the EE-induced intrahepatic cholestasis *** study revealed that significant changes in the maternal sera level of UCN,placental lev

关键词： cholestasis,intrahepatic Pregnancy Compensatory vasodilatory effect WFS1 CRH family peptides Ischemiareperfusion

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Dysregulated Hepatic Expression of Glucose Transporter Type-1,Toll-Like Receptor 4,and Nuclear Factor Kappa B in Estrogen-Induced cholestasis Pregnant Rats with Placental Ischemia-Reperfusion Stress

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Maternal-Fetal Medicine 2022年第1期4卷 17-23页

作者： Fan Zhou Huafang Chen Dan Shan Yuxia Wu Qian Chen Yayi Hu Department of Obstetrics and Gynecology West China Second University HospitalSichuan UniversityChengduSichuan 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University) Ministry of EducationChengduSichuan 610041China

Objective:This study aimed at investigating the expression of nuclear factor kappa B(NF-κB)and mammalian target of rapamycin(mTOR)related signal pathways in liver tissues of intrahepatic cholestasis of pregnancy animal ***:Estrogen(EE)-induced cholestasis and a placental ischemia-reperfusion(IR)model were established in pregnant *** pregnant rats were divided into four groups by random number table:EE-IR group(n=6),EE-sham group(n=6),control-IR group(n=6)and control-sham group(n=6).Liver expression of mTOR,its upstream regulator DNA damage response-1(REDD1),and downstream factor glucose transporter type-1(GLUT1),accompanied by NF-κB(p65 is the most important component),its activator toll-like receptor 4(TLR4),and inhibitor IκBα,were detected by western blot analysis and real-time polymerase chain *** intergroup comparisons were performed with a one-way analysis of variance,the comparisons among groups were analyzed with the nonparametric Kruskal-Wallis ***:Giving pregnant rats EE alone reduced the hepatic expression of IκBα(0.72±0.20vs.1.01±0.07,P=0.008).Meanwhile,giving pregnant rats placental IR alone increased liver levels of REDD1(3.24±0.98vs.1.06±0.24,P=0.025),GLUT1(2.37±0.82vs.1.09±0.10,P=0.039),TLR4(2.12±0.29vs.1.20±0.28,P=0.010),and p65(2.09±0.85vs.1.04±0.06,P=0.023),and decreased hepatic mTOR(0.50±0.07vs.1.01±0.03,P=0.001)and IκBα(0.61±0.08vs.1.01±0.07,P=0.014)*** EE-treated rats to placental IR did not further alter liver levels of GLUT1(2.02±0.45vs.1.79±0.39,P=0.240),TLR4(2.10±0.74vs.1.60±0.36,P=0.129),or p65(2.41±0.83vs.1.65±0.46,P=0.145),whereas it did decrease hepatic mTOR(0.42±0.09vs.0.90±0.14,P=0.008)and IκBα(0.43±0.09vs.0.72±0.20,P=0.004)expression and enhance REDD1 expression(4.46±0.65vs.2.05±0.47,P=0.009).Placental IR stress did impact the hepatic expression of REDD1-mTOR-GLUT1 and TLR4/NF-κB/IκBαin pregnant ***:Placental IR-induced hepatic GLUT1,TLR4,and p65 alternation,which respond

关键词： cholestasis,intrahepatic Liver mTOR Pregnancy

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