Impaired Compensatory Vasodilatory Effect Mediated by Wolfram Syndrome 1 and Corticotropin-Releasing Hormone Family Peptides in 17α-Ethynylestradiol-Induced Intrahepatic Cholestasis Pregnant Rats When Under Additional Acute Hypoxia Stress

作     者：Tingting Xu Daijuan Chen Xixi Deng Yongchi Zhan Fan Zhou Xiaodong Wang 

作者机构：Department of Obstetrics and GynecologyWest China Second University HospitalSichuan UniversitySichuan 610041China Key Laboratory of Birth Defects and Related Diseases of Women and Children(Sichuan University)Ministry of EducationSichuan 610041China 

出 版 物：《Maternal-Fetal Medicine》 (母胎医学杂志（英文）)

年 卷 期：2022年第4卷第1期

页      面：7-16页

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.81571446) the National Natural Science Foundation for Young Scientists of China(Grant No.82001560) 

主　　题：Cholestasis,intrahepatic Pregnancy Compensatory vasodilatory effect WFS1 CRH family peptides Ischemiareperfusion 

摘      要：Objective:To investigate the possible regulatory mechanism of corticotropin-releasing hormone(CRH),urocortin(UCN),and Wolfram syndrome 1(WFS1)in 17α-ethynylestradiol(EE)-induced intrahepatic cholestasis pregnant rats and its ischemia reperfusion(IR)***:Pregnant rats(n=60)were randomly divided into four experimental groups by random number table(Control,EE,IR,and EE-IR groups),and were studied on the 17^(th),19th,and 21st gestational days(GD)(n=5 in each group at the indicated time).Growth and development indicators of fetal rats among these four groups were ***-linked immunosorbent assay was employed to detect CRH,UCN,and WFS1 levels in maternal *** blotting and real-time polymerase chain reaction were used to quantify placental protein and placental mRNA levels of CRH,UCN,and *** analysis of variance and least significant difference test were used to establish the group and individual ***:A significant difference was found in placenta weight(F=8.10,P0.05),and IR(0.46±0.15vs.0.22±0.15,P0.05)groups at 19th GD,indicating that endoplasmic reticulum stress may be ***,the expression of CRH(0.42±0.05vs.0.58±0.12,P0.05),and WFS1(0.57±0.07vs.0.74±0.12,P0.05)protein in the EE-IR group was subsided compared to the IR group at 17^(th) ***:Fetal rat growth restriction was found in the EE-induced intrahepatic cholestasis *** study revealed that significant changes in the maternal sera level of UCN,placental lev