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Exogenous melatonin improves the chilling tolerance and preharvest fruit shelf life in eggplant by affecting ROS-and senescence-related processes

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Horticultural Plant Journal 2023年第3期9卷 523-540页

作者： Lijun Song Zhuo Tan Weiwei Zhang Qiang Li Zhaoxiang Jiang Shuxing Shen Shuangxia Luo Xueping Chen State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Vegetable Germplasm Innovation and Utilization of HebeiCollaborative Innovation Center of Vegetable Industry in HebeiCollege of HorticultureHebei Agricultural UniversityBaodingHebei 071000China College of Life Sciences Hebei Agricultural UniversityBaodingHebei 071000China

Low temperature is the most common abiotic stress factors during the eggplant cultivation in solar *** plays important roles in plant resistance to low ***,the role of melatonin in regulating chilling tolerance and extending the preharvest shelf life of eggplant fruits is still *** this study,we investigated the effects of exogenous melatonin on eggplant plants and fruits in response to low *** simulated low-temperature conditions,exogenous melatonin significantly relieved the chilling symptoms of seedlings by reducing reactive oxygen species (ROS) and malondialdehyde (MDA) levels and relative leakage *** reductions were caused by higher superoxide dismutase (SOD) and catalase (CAT) activities and increased endogenous polyamine and melatonin levels compared with those in untreated ***,the expression levels of SOD,CAT1/2,and polyamine synthesis genes (ADC and ODC) were also increased by 100μmol·L~(-1)melatonin,as well as those of genes involved in melatonin synthesis (TDC,T5H,SNAT,ASMT,and COMT) and cold regulation (COR1,CBFa/b,and ZAT2/6/12).To further investigate the effects of melatonin on eggplant leaves and fruits under natural low temperature conditions,100μmol·L~(-1)melatonin was sprayed on the functional leaves at three days before commodity *** significantly alleviated chilling injury in the leaves and pericarp and extended the preharvest shelf life of the fruit by increasing the expression of COR1,CBF,ZAT2/6/12,and API5 and decreasing the expression of senescence-related genes (NCED1/2 and SAG12).Therefore,100μmol·L~(-1)melatonin improved chilling tolerance and fruit shelf life by upregulating ZAT2/6/12 to affect ROS-and senescence-related processes,which provides a reference for alleviating cold stress and extending the preharvest fruit shelf life in eggplant.

关键词： Eggplant Melatonin chilling tolerance Cold regulation Reactive oxygen species Preharvest fruit shelf life

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chilling tolerance of Cucumber During Germination is Related to Expression of Lysine Decarboxylase Gene

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Agricultural Sciences in China 2005年第12期4卷 898-902页

作者： LU Ming-hui LI Xiao-ming CHEN Jin-feng CHEN Long-zheng QIAN Chun-tao National Key Laboratory of Crop Genetics and Germplasm Enhancement College of Horticulture Nanjing Agricultural UniversityNanjing 210095 P.R.China

Using cDNA-AFLP technique, a specific fragment was isolated from cucumber cultivar Changchun mici possessing chilling tolerance induced at low temperature （15℃）. This fragment, named cctr 132, could not be induced in the chilling sensitive cucumber cultivar Beijing jietou. After recovering the fragment, sequencing and translating, the results of blastx and blastp in GenBank of NCBI indicated that CCTR132 had 88.37% identities and 100% positives with Oryza sativa putative lysine decarboxylase-like protein respectively, and PGGXGTXXE, the putative conserved domain of lysine decarboxylase family, was detected from CCTR132, suggesting the cucumber chilling tolerance during germination is related to the expression of the lysine decarboxylase gene.

关键词： Cucumber chilling tolerance cDNA-AFLP Lysine decarboxylase Cadaverine

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chilling tolerance and Physiological Parameters as Influenced by Grafting in Watermelon Seedlings

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Agricultural Sciences in China 2003年第10期2卷 1164-1169页

作者： LIUHui-ying ZHUZhu-jun LUGuo-hua QIANQiong-qiu InstituteofVegetableScience ZhejiangUniversityHangzhou310029P.R.China DepartmentofHorticulture XinjiangShiheziUniversityShihezi832003P.R.China

The influences of different rootstocks on chilling tolerance and physiological parameters in watermelon seedlings have been studied. The results showed that grafting improved the chilling tolerance. Compared with own-rooted watermelon seedlings, the grafted watermelon seedlings had lower chilling injury index, lower electrolytic leakage (%), lower malondialdehyde (MDA) content, higher chlorophyll and proline content, and higher activities of superoxide dismutase (SOD), ascorbate peroxidase (AsA-POD) and dehydroascorbate reductase (DR) in the leaves under low temperature stress. There was a considerable difference of chilling tolerance among different grafted watermelon seedlings due to the difference of rootstock chilling tolerance. After low temperature treatment, the grafted seedling with higher chilling tolerance had lower electrolytic leakage (%),lower MDA content, higher proline content and higher activities of SOD, AsA-POD and DR in the leaves compared with the grafted seedling with weaker chilling tolerance. From these, we could conclude that chilling tolerance of watermelon seedlings may be related to higher antioxidative ability and membrane stability in the plants. The chilling tolerance of grafted seedling could be properly evaluated by comprehensive physiological indexes but not a single physiological index.

关键词： Watermelon Low temperature stress Grafted seedling chilling tolerance

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Genome-wide Association Analysis of Ten chilling tolerance Indices at the Germination and Seedling Stages in Maize

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Journal of Integrative Plant Biology 2013年第8期55卷 735-744页

作者： Juan Huang Jianhua Zhang Wenzhen Li Wei Hu Lichao Duan Yang Feng Fazhan Qiu Bing Yue National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Maize seedlings are very sensitive to chilling, especially during the transition phase from heterotrophic to autotrophic growth. Genetic dissection of the genetic basis of chilling tolerance would provide useful information for genetic improvement of maize inbreds. In this study, genome-wide association analysis was conducted to explore the genetic architecture of maize chilling tolerance at the seed germination and seedling stages with an association panel of 125 inbreds. Ten tolerance indices （ratios of the performance of 10 germination rates and seedling growth-related traits under chilling stress and control conditions） were investigated to assess the ability of chilling tolerance of the inbreds, and a total of 43 single nucleotide polymorphisms associated with chilling tolerance were detected, with none of them being related to chilling tolerance at both the germination and seedling stages simultaneously. Correlation analysis also revealed that the genetic basis of chilling tolerance at the seed germination stage is generally different from that at the seedling stage. In addition, a total of 40 candidate genes involving 31 of the 43 single nucleotide polymorphisms were predicted, and were grouped into five categories according to their functions. The possible roles of these candidate genes in chilling tolerance were also discussed.

关键词： Association mapping chilling tolerance germination and seedling stage maize.

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Ectopic expression of antifreeze protein gene from Ammopiptanthus nanus confers chilling tolerance in maize

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The Crop Journal 2021年第4期9卷 924-933页

作者： Yuanyuan Zhang Yang Cao Hongying Zheng Wenqi Feng Jingtao Qu Fengling Fu Wanchen Li Haoqiang Yu Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of AgricultureMaize Research InstituteSichuan Agricultural UniversityChengdu 611130SichuanChina College of Life Science&Biotechnology Mianyang Teachers’CollegeMianyang 621000SichuanChina

Improved chilling tolerance is important for maize production. Previous efforts in transgenics and marker-assisted breeding have not achieved practical results. In this study, the antifreeze protein(AnAFP) from the super-xerophyte Ammopiptanthus nanus was aligned to KnS-type *** in vitro and subcellular localization showed that AnAFP was phosphorylated by maize casein kinase II and translocated from nucleus to cytoplasm under chilling stress. AnAFP also increased lactate dehydrogenase activity. A parent line of commercial maize hybrids was transformed with the AnAFP gene. Based on thermal asymmetric interlaced PCR, one hemizygous and two homozygous integration sites were identified in one T_(1) line. Ectopic expression of AnAFP in transgenic lines was confirmed by quantitative real-time PCR, RNA-seq, and Western blotting. After chilling treatment, the transgenic lines showed significantly improved phenotype, higher kernel production, survival rate and biomass, and lower relative electrolyte leakage and superoxide dismutation than the untransformed line. Thus, ectopic expression of AnAFP gene improved chilling tolerance in the transgenic lines, which could be used to apply for further safety assessment for commercial breeding.

关键词： Antifreeze protein chilling tolerance Dehydrin Ectopic expression Homozygosity identification

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Transcriptional activation and phosphorylation of OsCNGC9 confer enhanced chilling tolerance in rice

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Molecular Plant 2021年第2期14卷 315-329页

作者： Jiachang Wang Yulong Ren Xi Liu Sheng Luo Xiao Zhang Xin Liu Qibing Lin Shanshan Zhu Hua Wan Yang Yang Yu Zhang Bin Lei Chunlei Zhou Tian Pan Yongfei Wang Mingming Wu Ruonan jing Yang Xu Meng Han Fuqing Wu Cailin Lei Xiuping Guo Zhijun Cheng Xiaoming Zheng Yihua Wang Zhigang Zhao Ling Jiang Xin Zhang Yong-Fei Wang Haiyang Wang Jianmin Wan National Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China MOE Key Laboratory of Bioinformatics School of Life SciencesTsinghua UniversityBeijing100084China

Low temperature is a major environmental factor that limits plant growth and *** transient elevation of cytoplasmic calcium has long been recognized as a critical signal for plant cold tolerance,the calcium channels responsible for this process have remained largely *** we report that OsCNGC9,a cyclic nucleotide-gated channel,positively regulates chilling tolerance by mediating cytoplasmic calcium elevation in rice(Oryza sativa).We showed that the loss-of-function mutant of OsCNGC9 is defective in cold-induced calcium influx and more sensitive to prolonged cold treatment,whereas OsCNGC9 overexpression confers enhanced cold ***,we demonstrated that in response to chilling stress,OsSAPK8,a homolog of Arabidopsis thaliana OST1,phosphorylates and activates OsCNGC9 to trigger Ca2+***,we found that the transcription of OsCNGC9 is activated by a rice dehydration-responsive element-binding transcription factor,*** together,our results suggest that OsCNGC9 enhances chilling tolerance in rice through regulating cold-induced calcium influx and cytoplasmic calcium elevation.

关键词： OsCNGC9 OsSAPK8 OsDREB1A cold signaling transduction chilling tolerance

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The Relationship Between Heat-induced chilling tolerance and Endogenous ABA Levels in Mangoes

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Agricultural Sciences in China 2002年第5期1卷 568-571页

作者： ZHU Shi-jiang and JI Zuo-liang( College of Horticulture , South China Agricultural University, Guangzhou 510642 ,P.R. China ) College of Horticulture South China Agricultural University Guangzhou 510642 P. R. China

Heating 'Zhihua' mangoes (Mangifera indica L. cv. Zhihua) in hot air at 38℃ for 12hours significantly reduced the development of chilling injury following storage for 12days at 2t. Prolonging the heating up to 72hours acquired similar results. During the 12 days' storage at 2℃ following heating for 72hours, the membrane leakage of the mango peels showed little change, while it increased with time for the control fruits, and the endogenous ABA levels in the heated fruits were higher than that in the control. These results indicated that higher ABA rates were linked with stronger chilling tolerance in mangoes.

关键词： chilling tolerance ABA Heat treatment Mango

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Analysis and Evaluation Indicator Selection of chilling tolerance of Different Cotton Genotypes

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Agricultural Science & Technology 2012年第11期13卷 2338-2346页

作者：武辉侯丽丽周艳飞范志超石俊毅阿丽艳.肉孜张巨松新疆农业大学农学院/教育部棉花工程研究中心新疆乌鲁木齐830052

[Objectivc] This study aimed to investigate the chilling tolerance of seedlings of different cotton genotypes and screen appropriate indicators for assess- ing chilling tolerance, to establish reliable mathematical evaluation model for chilling tolerance of cotton, thus providing theoretical basis for breeding and promoting new chilling-tolerant cotton germplasms and large-scale evaluation of chilling tolerance of cotton varieties. [Method] Fifteen cotton varieties （lines） were used as experimental materials. The photosynthetic gas exchange parameters, chlorophyll fluorescence ki- netic parameters, chlorophyll content, relative soluble sugar content, malonaldehyde content, relative proiine content, relative conductivity and other 12 physiological indi- cators of seedling leaves under low temperature treatment （5 ℃, 12 h） and recovery treatment （25 ℃. 24 h） were determined; based on the chilling tolerance coefficient （CTC） of various individual indicators, the comprehensive evaluation of chilling toler- ance was conducled by using principal component analysis, hierarchical cluster anal- ysis and stepwise regression analysis. [Result] The results showed that the 12 indi- vidual physiological indicators could be classified into 7 independent comprehensive components by principal component analysis; 15 cotton varieties （lines） were clus- tered into three categories by using membership function method and hierarchical cluster analysis; the mathematical model for evaluating chilling tolerance of cotton seedlings was established： D =0.275 -0.244Fo1 ＋0.206Fv/Fm1＋0.326g,%-0.056SS ＋ 0.225MDA＋O.O38REC （FF=0.995）, and the evaluation accuracy of the equation was higher than 94.25%,0. Six identification indicators closely related to chilling tolerance were screened, including Fo,, Fv/Fm1, Seedling leaves of cotton varieties （lines） gs2, SS, MDA, and REC. [Conclusion] with high chilling tolerance are less dam- aged under low temperature stress, and are able to maintain relatively

关键词： Cotton chilling tolerance Principal components analysis Comprehensiveevaluation Stepwise regression analysis

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Effect of Polyamine Priming on chilling tolerance of Lolium perenne during Seed Imbibition

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Agricultural Science & Technology 2012年第9期13卷 1859-1863,1869页

作者：王应芬王普昶吴佳海赵丽丽莫本田黎俊贵州牧草种籽繁殖场贵州独山558202 贵州省草业研究所贵州贵阳550006 贵州大学动物科学学院贵州贵阳550025

[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.

关键词： Polyamines Annual Lofium perenne chilling tolerance Seed imbibition Antioxidant enzymes

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Genetic variation in a heat shock transcription factor modulates cold tolerance in maize

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MOLECULAR PLANT 2024年第9期17卷 1423-1438页

作者： Gao, Lei Pan, Lingling Shi, Yiting Zeng, Rong Li, Zhuoyang Zhang, Xuan Zhao, Xiaoming Gong, Xinru Huang, Wei Yang, Xiaohong Lai, Jinsheng Zuo, Jianru Gong, Zhizhong Wang, Xiqing Jin, Weiwei Dong, Zhaobin Yang, Shuhua China Agr Univ Frontiers Sci Ctr Mol Design Breeding Ctr Crop Funct Genom & Mol Breeding State Key Lab Plant Environm ResilienceColl Biol Beijing 100193 Peoples R China China Agr Univ Natl Maize Improvement Ctr Frontiers Sci Ctr Mol Design Breeding Dept Plant Genet & BreedingState Key Lab Maize Bi Beijing Peoples R China Chinese Acad Sci State Key Lab Plant Genom Inst Genet & Dev Biol Beijing 100101 Peoples R China Chinese Acad Sci Inst Genet & Dev Biol Natl Plant Gene Res Ctr Beijing 100101 Peoples R China

Understanding how maize (Zea mays) responds to cold stress is crucial for facilitating breeding programs of cold-tolerant varieties. Despite extensive utilization of the genome-wide association study (GWAS) approach for exploring favorable natural alleles associated with maize cold tolerance, few studies have successfully identified candidate genes that contribute to maize cold tolerance. In this study, we used a diverse panel of inbred maize lines collected from different germplasm sources to perform a GWAS on variations in the relative injured area of maize true leaves during cold stress-a trait very closely correlated with maize cold tolerance. We identified HSF21, which encodes a B-class heat shock transcription factor (HSF) that positively regulates cold tolerance at both the seedling and germination stages. Natural variations in the promoter of the cold-tolerant HSF21(Hap1) allele led to increased HSF21 expression under cold stress by inhibiting binding of the basic leucine zipper bZIP68 transcription factor, a negative regulator of cold tolerance. By integrating transcriptome deep sequencing, DNA affinity purification sequencing, and targeted lipidomic analysis, we revealed the function of HSF21 in regulating lipid metabolism homeostasis to modulate cold tolerance in maize. In addition, we found that HSF21 confers maize cold tolerance without incurring yield penalties. Collectively, this study establishes HSF21 as a key regulator that enhances cold tolerance in maize, providing valuable genetic resources for breeding of cold-tolerant maize varieties.

关键词： heat shock factor HSF21 cold tolerance natural variation bZIP68 lipid metabolism maize QUANTITATIVE TRAIT LOCI chilling tolerance FREEZING tolerance LIPID CHANGES STRESS MEMBRANE ARCHITECTURE IMPACTS PLANTS YIELD

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