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Hyperbaric oxygen protects against PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of cell apoptosis and autophagy

引用

BIOCELL 2022年第1期46卷 137-148页

作者： JIANRONG YANG WAN CHEN XING ZHOU YAOXUAN LI ZHIHUANG NONG LIYUAN ZHOU XUAN WEI XIAORONG PAN CHUNXIA CHEN WENSHENG LU Hepatobiliary Surgery Guangxi Academy of Medical SciencesThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Emergency The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Endocrinology The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Neurology The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Pharmacy The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Hyperbaric Oxygen The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Research Center of Medical Sciences The People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China

In this study,we investigated the protective effect of hyperbaric oxygen(HBO)on PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion and its possible ***12 and H9C2 cell oxygen-glucose deprivation/reperfusion model were *** were divided into a control group,model group,hyperbaric air(HBA)group and HBO *** cell viability was detected by the CCK8 *** 33342 and PI staining assays and mitochondrial membrane potential(MMP)assays were used to detect cell *** ultrastructure of cells,including autophagosomes,lysosomes,and apoptosis,were examined using a transmission electron *** expression of autophagy-related proteins was detected by cellular immunofluorescence and *** results showed that HBO can significantly improve the vitality of damaged PC12 and H9C2 cells caused by oxygen–glucose deprivation/*** can significantly inhibit apoptosis of PC12 and H9C2 cells caused by oxygenglucose deprivation/***,we found that the protective mechanism of PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion may be related to the inhibition of the autophagy *** this study,the results of cellular immunofluorescence and immunocytochemistry experiments showed that the 4E-BP1,p-AKt and mTOR levels of PC12 and H9C2 cells in the model group decreased,while the levels of LC3B,Atg5 and p53 ***,after HBO treatment,these autophagy-related indexes were *** addition,observation of the cell ultrastructure with transmission electron microscopy found that in the model group,a significant increase in the number of autophagic vesicles was *** the HBO group,a decrease in autophagic vesicles was *** study demonstrated that hyperbaric oxygen protects against PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of cell apoptosis and autophagy.

关键词： Hyperbaric oxygen PC12 cells H9C2 cells celoxygen-glucose deprivation/reperfusion Apoptosis Autophagy

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