Hyperbaric oxygen protects against PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of cell apoptosis and autophagy

作     者：JIANRONG YANG WAN CHEN XING ZHOU YAOXUAN LI ZHIHUANG NONG LIYUAN ZHOU XUAN WEI XIAORONG PAN CHUNXIA CHEN WENSHENG LU 

作者机构：Hepatobiliary SurgeryGuangxi Academy of Medical SciencesThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of EmergencyThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of EndocrinologyThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of NeurologyThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of PharmacyThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Hyperbaric OxygenThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China Department of Research Center of Medical SciencesThe People’s Hospital of Guangxi Zhuang Autonomous RegionNanning530021China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2022年第46卷第1期

页      面：137-148页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(81960246,81701089,81560044 and 30860113) the Guangxi Natural Science Foundation(2020GXNSFAA238003 and 2017GXNSFBA198010) the Guangxi Medical and Health Appropriate Technology Research and Development Project(S2020076,S201422-01 and S2019087) the Shanxi Health Research Project(2019165) 

主　　题：Hyperbaric oxygen PC12 cells H9C2 cells Celoxygen-glucose deprivation/reperfusion Apoptosis Autophagy 

摘      要：In this study,we investigated the protective effect of hyperbaric oxygen(HBO)on PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion and its possible ***12 and H9C2 cell oxygen-glucose deprivation/reperfusion model were *** were divided into a control group,model group,hyperbaric air(HBA)group and HBO *** cell viability was detected by the CCK8 *** 33342 and PI staining assays and mitochondrial membrane potential(MMP)assays were used to detect cell *** ultrastructure of cells,including autophagosomes,lysosomes,and apoptosis,were examined using a transmission electron *** expression of autophagy-related proteins was detected by cellular immunofluorescence and *** results showed that HBO can significantly improve the vitality of damaged PC12 and H9C2 cells caused by oxygen–glucose deprivation/*** can significantly inhibit apoptosis of PC12 and H9C2 cells caused by oxygenglucose deprivation/***,we found that the protective mechanism of PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion may be related to the inhibition of the autophagy *** this study,the results of cellular immunofluorescence and immunocytochemistry experiments showed that the 4E-BP1,p-AKt and mTOR levels of PC12 and H9C2 cells in the model group decreased,while the levels of LC3B,Atg5 and p53 ***,after HBO treatment,these autophagy-related indexes were *** addition,observation of the cell ultrastructure with transmission electron microscopy found that in the model group,a significant increase in the number of autophagic vesicles was *** the HBO group,a decrease in autophagic vesicles was *** study demonstrated that hyperbaric oxygen protects against PC12 and H9C2 cell damage caused by oxygen-glucose deprivation/reperfusion via the inhibition of cell apoptosis and autophagy.