检索结果-南通市图书馆

Oncology Research 2024年第9期32卷 1439-1452页

作者： PEILING WU LIFANG ZHAO HONGYAN ZHANG YUEYAN LOU DONGFANG CHEN SHAN XUE XUEQING LIU HANDONG JIANG Department of Respiratory and Critical Care Medicine Renji HospitalShanghai Jiao Tong University School of MedicineShanghai200127China

Objectives:The pro-oncogenic effects of NCAPD2 have been extensively studied across various tumor types;however,its precise role within the context of lung adenocarcinoma(LUAD)remains *** study aims to elucidate the biological functions of NCAPD2 in LUAD and unravel the underlying mechanistic ***:Utilizing bioinformatics methodologies,we explored the differential expression of NCAPD2 between normal and tumor samples,along with its correlations with clinical-pathological characteristics,survival prognosis,and immune ***:In the TCGA-LUAD dataset,tumor samples demonstrated significantly elevated levels of NCAPD2 expression compared to normal samples(pcell *** infiltration analysis revealed that NCAPD2 expression levels were linked to the infiltration of memory B cells,naïve CD4+T cells,activated memory CD4+T cells,and M1 *** vitro experiments demonstrated that silencing NCAPD2 suppressed LUAD cell proliferation,migration,invasion,epithelial-mesenchymal transition(EMT),and cell cycle ***:In summary,NCAPD2 may represent a promising prognostic biomarker and novel therapeutic target for LUAD.

关键词： NCAPD2 LUAD Prognosis Immune infiltration cell cycle

cell cycle dysregulation with overexpression of KIF2C/MCAK is a critical event in

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Genes & Diseases 2023年第1期10卷 212-227页

作者： Xiaofeng Zuo Peixin Meng Yuxin Bao Chuntao Tao Yitao Wang Xianjun Liu Youquan Bu Jiang Zhu Department of Otolaryngology The First Affiliated Hospital of Chongqing Medical UniversityChongqing Medical UniversityChongqing 400016China Department of Biochemistry and Molecular Biology Chongqing Medical UniversityChongqing 400016China Molecular Medicine and Cancer Research Center Chongqing Medical UniversityChongqing 400016China

Nasopharyngeal carcinoma(NPC)is a common malignant carcinoma of the head and neck,and the biological mechanisms underlying the pathogenesis of NPC remain not fully *** the present study,we systematically analyzed four independent NPC transcriptomic datasets and focused on identifying the critical molecular networks and novel key hub genes implicated in *** found totally 170 common overlapping differentially expressed genes(DEGs)in the four NPC *** and KEGG pathway analysis revealed that cell cycle dysregulation is a critical event in ***-protein interaction(PPl)network analysis identified a 15 hub-gene corenetwork with overexpressedkinesin family member2C(KIF2C)as a central ***-of-function study demonstrated that knockdown of KiF2C significantly inhibited cell growth and cell motility,and delayed cell cycle progression,accompanied with dramatic mitotic defects in spindle formation in NPC ***-seq analysis revealed that KIF2c knockdown led to deregulation of various downstream ***2C could also regulate the AKT/mTOR pathways,and enhance paclitaxel sensitivity in NPC *** together,our results suggest that cell cycle dysregulation is a critical event during NPC pathogenesis and KIF2C is a novel key mitotic hub gene with therapeutic potential in NPC.

关键词： Bioinformatics cell cycle KIF2C Mitosis Nasopharyngeal carcinoma

Melatonin improves the first cleavage of parthenogenetic embryos from vitrified-warmed mouse oocytes potentially by promoting cell cycle progression

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Animal Science and Biotechnology 2022年第1期13卷 106-122页

作者： Bo Pan Izhar Hyder Qazi Shichao Guo Jingyu Yang Jianpeng Qin Tianyi Lv Shengqin Zang Yan Zhang Changjun Zeng Qingyong Meng Hongbing Han Guangbin Zhou Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province College of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Department of Veterinary Anatomy and Histology Shaheed Benazir Bhutto University of Veterinary and Animal SciencesSakrandSindh 67210Pakistan State Key Laboratory of AgroBiotechnology China Agricultural UniversityBeijing 100193China National Engineering Laboratory for Animal Breeding Key Laboratory of Animal Genetics and Breeding of the Ministry of AgricultureBeijing Key Laboratory for Animal Genetic ImprovementCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China

Background:This study investigated the effect of melatonin(MT)on cell cycle(G1/S/G2/M)of parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II(MII)oocytes and elucidated the potential mechanism of MT action in the first cleavage of ***:After vitrification and warming,oocytes were parthenogenetically activated(PA)and in vitro cultured(IVC).Then the spindle morphology and chromosome segregation in oocytes,the maternal mRNA levels of genes including Miss,Doc1r,Setd2 and Ythdf2 in activated oocytes,pronuclear formation,the S phase duration in zygotes,mitochondrial function at G1 phase,reactive oxygen species(ROS)level at S phase,DNA damage at G2 phase,early apoptosis in 2-cell embryos,cleavage and blastocyst formation rates were *** results indicated that the vitrification/warming procedures led to following perturbations 1)spindle abnormalities and chromosome misalignment,alteration of maternal mRNAs and delay in pronucleus formation,2)decreased mitochondrial membrane potential(MMP)and lower adenosine triphosphate(ATP)levels,increased ROS production and DNA damage,G1/S and S/G2 phase transition delay,and delayed first cleavage,and 3)increased early apoptosis and lower levels of cleavage and blastocyst *** results further revealed that such negative impacts of oocyte cryopreservation could be alleviated by supplementation of warming,recovery,PA and IVC media with 10^(−9) mol/L MT before the embryos moved into the 2-cell stage of ***:MT might promote cell cycle progression via regulation of MMP,ATP,ROS and maternal mRNA levels,potentially increasing the first cleavage of parthenogenetic zygotes developed from vitrified-warmed mouse oocytes and their subsequent development.

关键词： cell cycle Cleavage rate Melatonin MetaphaseⅡoocyte Parthenogenetic activation Vitrification

Antrodia cinnamomea exerts an anti-hepatoma effect by targeting PI3K/AKT-mediated cell cycle progression in vitro and in vivo

在线全文

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2022年第2期12卷 890-906页

作者： Yan Zhang Pin Lv Junmei Ma Ning Chen Huishan Guo Yan Chen Xiaoruo Gan Rong Wang Xuqiang Liu Sufang Fan Bin Cong Wenyi Kang Hebei Key Laboratory of Forensic Medicine College of Forensic MedicineHebei Medical UniversityShijiazhuang 050017China Hebei Food Safety Key Laboratory Hebei Food Inspection and Research InstituteShijiazhuang 050091China Cardiovascular Medical Science Center Department of Cell BiologyHebei Medical UniversityShijiazhuang 050017China National R&D Center for Edible Fungus Processing Technology Henan UniversityKaifeng 475004China Research Unit of Digestive Tract Microecosystem Pharmacology and Toxicology Chinese Academy of Medical SciencesBeijing 100730China

Antrodia cinnamomea is extensively used as a traditional medicine to prevention and treatment of liver ***,its comprehensive chemical fingerprint is uncertain,and the mechanisms,especially the potential therapeutic target for anti-hepatocellular carcinoma(HCC)are still *** UPLC-Q-TOF/MS,139 chemical components were identified in *** dropping pills(ACDPs).Based on these chemical components,network pharmacology demonstrated that the targets of active components were significantly enriched in the pathways in cancer,which were closely related with cell proliferation ***,HCC data was downloaded from Gene Expression Omnibus database(GEO).The Cancer Genome Atlas(TCGA)and Dis Ge NET were analyzed by bioinformatics,and 79 biomarkers were ***,nine targets of ACDP active components were revealed,and they were significantly enriched in PI3 K/AKT and cell cycle signaling *** affinity between these targets and their corresponding active ingredients was predicted by molecular ***,in vivo and in vitro experiments showed that ACDPs could reduce the activity of PI3 K/AKT signaling pathway and downregulate the expression of cell cycle-related proteins,contributing to the decreased growth of liver ***,PI3 K/AKT-cell cycle appears as the significant central node in anti-liver cancer of ***.

关键词： Antrodia cinnamomea UPLC-Q-TOF/MS Anti-hepatocellular carcinoma Network pharmacology PI3K/AKT cell cycle

CUDC-101 as a dual-target inhibitor of EGFR and HDAC enhances the anti-myeloma effects of bortezomib by regulating G2/M cell cycle arrest

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第5期24卷 442-454页

作者： Wen CAO Shunnan YAO Anqi LI Haoguang CHEN Enfan ZHANG Liqin CAO Jinna ZHANG Yifan HOU Zhenfeng DAI Jing CHEN Xi HUANG Li YANG Zhen CAI Bone Marrow Transplantation Center the First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310006China Institute of Hematology Zhejiang UniversityHangzhou 310058China School of Medicine Zhejiang UniversityHangzhou 310058China

CUDC-101,an effective and multi-target inhibitor of epidermal growth factor receptor(EGFR),histone deacetylase(HDAC),and human epidermal growth factor receptor 2(HER2),has been reported to inhibit many kinds of cancers,such as acute promyelocytic leukemia and non-Hodgkin's ***,no studies have yet investigated whether CUDC-101 is effective against ***,we proved that CUDC-101 effectively inhibits the proliferation of multiple myeloma(MM)cell lines and induces cell apoptosis in a time-and dose-dependent ***,CUDC-101 markedly blocked the signaling pathway of EGFR/phosphoinositide-3-kinase(PI3K)and HDAC,and regulated the cell cycle G2/M ***,we revealed through in vivo experiment that CUDC-101 is a potent anti-myeloma *** is one of the important drugs in MM treatment,and we investigated whether CUDC-101 has a synergistic or additive effect with *** results showed that this drug combination had a synergistic anti-myeloma effect by inducing G2/M phase ***,our findings revealed that CUDC-101 could act on its own or in conjunction with bortezomib,which provides insights into exploring new strategies for MM treatment.

关键词： CUDC-101 Multiple myeloma Bortezomib Epidermal growth factor receptor(EGFR) cell cycle

Discovery of small molecule degraders for modulating cell cycle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2023年第5期17卷 823-854页

作者： Liguo Wang Zhouli Yang Guangchen Li Yongbo Liu Chao Ai Yu Rao MOE Key Laboratory of Protein Sciences School of Pharmaceutical SciencesMOE Key Laboratory of Bioorganic Phosphorus Chemistry&Chemical BiologyTsinghua UniversityBeijing100084China Beijing Tsinghua Changgung Hospital School of Clinical MedicineTsinghua UniversityBeijing102218China

The cell cycle is a complex process that involves DNA replication,protein expression,and cell *** of the cell cycle is associated with various ***-dependent kinases(CDKs)and their corresponding cyclins are major proteins that regulate the cell *** contrast to inhibition,a new approach called proteolysis-targeting chimeras(PROTACs)and molecular glues can eliminate both enzymatic and scaffold functions of CDKs and cyclins,achieving targeted *** field of PROTACs and molecular glues has developed rapidly in recent *** this article,we aim to summarize the latest developments of CDKs and cyclin protein *** selectivity,application,validation and the current state of each CDK degrader will be ***,possible methods are discussed for the development of degraders for CDK members that still lack ***,this article provides a comprehensive summary of the latest advancements in CDK and cyclin protein degraders,which will be helpful for researchers working on this topic.

关键词： PROTAC molecular glue degrader cell cycle CDK cyclin

Expression dynamics of periodic transcripts during cancer cell cycle progression and their correlation with anticancer drug sensitivity

在线全文

学校读者我要写书评

暂无评论

军事医学研究:英文版 2023年第4期10卷 444-460页

作者： Chun‑Xiao Li Jin‑Song Wang Wen‑Na Wang Dong‑Kui Xu Yan‑Tong Zhou Fang‑Zhou Sun Yi‑Qun Li Feng‑Zhu Guo Jia‑Lu Ma Xue‑Yan Zhang Meng‑Jiao Chang Bing‑He Xu Fei Ma Hai‑Li Qian State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of Medical Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China Department of VIP National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021China

Background:The cell cycle is at the center of cellular activities and is orchestrated by complex regulatory mechanisms,among which transcriptional regulation is one of the most important *** splicing dramatically expands the regulatory network by producing transcript isoforms of genes to exquisitely control the cell ***,the patterns of transcript isoform expression in the cell cycle are *** targeting cell cycle checkpoints are commonly used as anticancer therapies,but none of them have been designed or evaluated at the alternative splicing transcript *** utility of these transcripts as markers of cell cycle-related drug sensitivity is still unknown,and studies on the expression patterns of cell cycle-targeting drug-related transcripts are also ***:To explore alternative splicing patterns during cell cycle progression,we performed sequential transcriptomic assays following cell cycle synchronization in colon cancer HCT116 and breast cancer MDA-MB-231 cell lines,using flow cytometry and reference cell cycle transcripts to confirm the cell cycle phases of samples,and we developed a new algorithm to describe the periodic patterns of transcripts fluctuating during the cell *** of Drug Sensitivity in Cancer(GDSC)drug sensitivity datasets and Cancer cell Line Encyclopedia(CCLE)transcript datasets were used to assess the correlation of genes and their transcript isoforms with drug *** identified transcripts associated with typical drugs targeting cell cycle by determining correlation *** assays were used to confirm the effect of ENST00000257904 against cyclin dependent kinase 4/6(CDK4/6)***,alternative splicing transcripts associated with mitotic(M)phase arrest were analyzed using an RNA synthesis inhibition assay and transcriptome ***:We established high-resolution transcriptome datasets of synchronized cell cycle samples from colon cancer HCT11

关键词： cell cycle Alternative splicing Transcriptome Drug resistance Cyclin dependent kinase 4/6 inhibitor,Dolichyl-phosphate mannosyltransferase polypeptide 2

Wild pink bayberry free phenolic extract induces mitochondria-dependent apoptosis and G0/G1 cell cycle arrest through p38/MAPK and PI3K/Akt pathway in MDA-MB-231 cancer cells

在线全文

学校读者我要写书评

暂无评论

Food Science and Human Wellness 2023年第5期12卷 1510-1518页

作者： Wen Xia Ersheng Gong Yanyun Lin Bisheng Zheng Wenhan Yang Tong Li Sheng Zhang Peng Li Ruihai Liu Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health(111 Center) School of Food Science and EngineeringSouth China University of TechnologyGuangzhou 510641China School of Public Health and Health Management Gannan Medical UniversityGanzhou 341000China Key Laboratory of Tropical Crop Products Processing of Ministry of Agriculture and Rural Affairs Agricultural Products Processing Research InstituteChinese Academy of Tropical Agricultural SciencesZhanjiang 524001China Department of Food Science Cornell UniversityIthaca 14853USA Stem cell Research and cellular Therapy Center Affi liated Hospital of Guangdong Medical UniversityZhanjiang 524001China

Polyphenol-rich foods have been shown to be good for cancer prevention as powerful antioxidants. In this study, the mechanisms of wild pink bayberry free phenolic extract(WPBFE)inhibiting the proliferation and inducing apoptotic of MDA-MB-231 breast cancer cells was examined. The main phenolic acids and flavonols in WPBFE were gallic acid((18.83 ± 0.44)μg/g FW)and myricetin((1.52 ± 0.05)μg/g FW), respectively. The maximum inhibition rate of WPBFE at non-cytotoxicity dose(below 80 mg/mL)was 81%. Western blotting analysis showed that WPBFE could cause the arrest of cell cycle in G0/G1 phase by down-regulating expression levels of PCNA, CDK4, cyclin D1 and up-regulating the expression level of p21. Meanwhile, WPBFE induced apoptosis through initiating the mitochondrial death pathway by up-regulating cleaved caspase-3 and enhancing the ratio of Bax/Bcl-2, with the maximum expression levels of 1.29 and 2.03 folds that of control group, respectively. Further study of the upstream protein, we found that WPBFE down-regulated TRAF2, while upregulated p-ASK1, p-p38 and p-p53. Furthermore, WPBFE could down-regulate the expression of p-PI3K and p-Akt. These observations indicated that WPBFE might play an anticancer role through regulating the p38 MAPK together with PI3K/Akt pathway.

关键词： Phenolic extract Breast cancer cell cycle Apoptosis cell proliferation

E2F4 regulates the cell cycle and DNA replication in the silkworm,Bombyx mori

在线全文

学校读者我要写书评

暂无评论

Insect Science 2022年第4期29卷 1006-1016页

作者： Peng Chen Ling Wang Yan-Bi Long Guang-Yan Liang Xiu Yang Zhan-Qi Dong Xia Jiang Yan Zhu Min-Hui Pan Cheng Lu State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqingChina Key Laboratory of Sericultural Biology and Genetic Breeding Ministry of Agriculture and Rural AffairsSouthwest UniversityChongqingChina

The E2F family of transcription factors is crucial for cell cycle progression and cell fate *** E2Fs have been widely studied in mammals,there have been few studies performed in ***,we determined the function of E2F4 in the silkworm,Bombyx *** demonstrate that E2F proteins are highly conserved among species from lower animals to higher *** of the BmE2F4 gene led to cell cycle arrest in the G1 phase,whereas interfering with the BmE2F4 mRNA led to accumulation of cells in the S *** results indicate that BmE2F4 is important in cell cycle *** also demonstrate that the BmE2F4 gene is involved in DNA replication of BmN-SWU1 cells and DNA synthesis in the silk ***,we identified a protein called Bm14-3-3ζthat can interact with BmE2F4 and allow it to localize in the *** of the Bm14-3-3ζgene led to cell cycle arrest in the G1 phase,while knocking down the gene increased the proportion of cells in S *** findings provide important insights into the function of E2F transcription factors and increase our understanding of their involvement in cell cycle regulation.

关键词： Bm14-3-35 Bombyx mori cell cycle E2F transcription factors E2F4 insect

Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Medical Journal 2022年第4期135卷 447-455页

作者： Keke Li Jingkai Xu Ke Xue Ruixing Yu Chengxu Li Wenmin Fei Xiaoli Ning Yang Han Ziyi Wang Jun Shu Yong Cui Department of Dermatology China-Japan Friendship HospitalBeijing 100029China Graduate School of Peking Union Medical College Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Institute of Clinical Medical Science China-Japan Friendship HospitalBeijing 100029China

Background:Systemic lupus erythematosus(SLE)is a complex autoimmune disease,and the mechanism of SLE is yet to be fully *** aim of this study was to explore the role of two-pore segment channel 2(TPCN2)in SLE ***:Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression ofTPCN2 in *** performed a loss-of-function assay by lentiviral construct in Jurkat and THP-1 *** ofTPCN2 were confirmed at the RNA level by qRT-PCR and protein level by Western *** Count Kit-8 and flow cytometry were used to analyze the cell proliferation,apoptosis,and cell cycle ofTPCN2-deficient *** addition,gene expression profile ofTPCN2-deficient cells was analyzed by RNA sequencing(RNA-seq).Results:TPCN2 knockdown with short hairpin RNA(shRNA)-mediated lentiviruses inhibited cell proliferation,and induced apoptosis and cell-cycle arrest of G2/M phase in both Jurkat and THP-1 *** analyzed the transcriptome of knockdown-TPCN2-Jurkat cells,and screened the differential genes,which were enriched for the G2/M checkpoint,complement,and interleukin-6-Janus kinase-signal transducer and activator of transcription pathways,as well as changes in levels of forkhead box O,phosphatidylinositol 3-kinase/protein kinase B/mechanistic target of rapamycin,and T cell receptor pathways;moreover,TPCN2 significantly influenced cellular processes and biological ***:TPCN2 might be a potential protective factor against SLE.

关键词： TPCN2 Systemic lupus erythematosus RNA sequencing analysis Apoptosis cell cycle