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Plasma Science and Technology 2017年第2期19卷 63-68页

作者： Yuyang WANG Cheng CHENG Peng GAO Shaopeng LI Jie SHEN Yan LAN Yongqiang YU Paul K CHU The first Affiliated Hospital of Anhui Medical University Hefei 230032People's Republic of China Institute of Plasma Physics Chinese Academy of SciencesHefei 230031People's Republic of China Department of Physics and Materials Science City University of Hong KongTatChee AvenueKowloonHong KongPeople's Republic of China

An atmospheric-pressure air plasma is employed to treat C6 glioma cells in vitro. To elucidate on the mechanism causing cell death and role of reactive species （RS） in the medium produced by the plasma, the concentration of the long-lived RS such as hydrogen peroxide, nitrate, and ozone in the plasma-treated liquid （phosphate-buffered saline solution） is measured. When vitamin C is added to the medium as a ROS quencher, the viability of C6 glioma cells after the plasma treatment is different from that without vitamin C. The results demonstrate that reactive oxygen species （ROS） such as H2O2, and O3 constitute the main factors for inactivation of C6 glioma cells and the reactive nitrogen species （RNS） may only play an auxiliary role in cell death.

关键词： atmospheric pressure air plasma reactive species cell death

Recent advances in plant immunity with cell death:A review

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Journal of Integrative Agriculture 2022年第3期21卷 610-620页

作者： YIN Jun-jie XIONG Jun XU Li-ting CHEN Xue-wei LI Wei-tao State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Rice Research InstituteSichuan Agricultural University at WenjiangChengdu 611130P.R.China

cell death is an important physiological phenomenon in *** can be programmed or *** cell death is usually induced by abiotic or biotic *** studies have shown that many proteins regulate both cell death and immunity in ***,we provide a review on the advances in plant immunity with cell death,especially the molecular regulation and underlying mechanisms of those proteins involved in both cell death and plant *** addition,we discuss potential approaches toward improving plant immunity without compromising plant growth.

关键词： cell death unprogrammed cell death programmed cell death hypersensitive response reactive oxygen species plant immunity resistance

Overexpression of SGR Results in Oxidative Stress and Lesion-mimic cell death in Rice Seedlings

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Journal of Integrative Plant Biology 2011年第5期53卷 375-387页

作者： Huawu Jiang Yaping Chen Meiru Li Xingian Xu Guojiang Wu Key Laboratory of-Plant Resou-rces Conservation and Sustainable Utilization South China Botanical Garden Chinese Academyof Sciences Guangzhou 510650 China

It is thought that the Stay Green Rice （SGR） gene is involved in the disaggregation of the light harvesting complex and in the subsequent breakdown of chlorophyll and apo-protein during senescence. In this study, we found that overexpression of SGR （Ov-SGR） resulted in the generation of singlet oxygen and other reactive oxygen species and produced a chlorophyll-dependent regional cell death phenotype on leaves of rice seedlings. Transcriptome analyses using Affymetrix Rice GeneChips revealed that Ov-SGR rice seedlings exhibited a number of signs of singlet oxygen response. The genes and their associated biochemical pathways identified provide an insight into how rice plants respond to singlet oxygen at the molecular and physiologic level.

关键词： PLANT gene expression OXIDATIVE stress cell death SEEDLINGS CHLOROPHYLL ACTIVE oxygen RICE GENETICS

Seasonal variations of group-specific phytoplankton cell death in Xiamen Bay, China

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Chinese Journal of Oceanology and Limnology 2017年第2期35卷 324-335页

作者：黄晓舟柳欣陈纪新肖武鹏曹振黄邦钦 Fujian Provincial Key Laboratory of Coastal Ecology and Environmental Studies Key Laboratory of Coastal and Wetland Ecosystems Ministry of EducationXiamen University College of Oceanography and Food Science Quanzhou Normal University

The importance of phytoplankton cell death is being increasingly recognized, however, there are still no published reports on this in Xiamen Bay. In this study, the proportion of dead phytoplankton cells associated with environmental factors was assessed at a station in Xiamen Bay from December 2012 to December 2013, using a cell digestion assay, which is an effective method to analyze dead/ living ceils in complex natural phytoplankton communities. The percentages of dead cells （% DC） in the total phytoplankton in summer （16%＋6%） were lower than those in winter （27%q-16%）. Six groups of phytoplankton （G1-G6） were categorized by flow cytometry. These phytoplankton communities with diverse seasonal variations in % DC had different responses to environmental constraints. The main factors affecting mortality were temperature and salinity, while nutrient concentration showed little influence on phytoplankton death. Additio^aally, our results provide evidence that chlorophyll a concentrations had an inverse relationship with total phytoplankton % DC and viable cell abundance was more meaningful than total cells to explain variations in environmental parameters （such as Chl a）. Moreover, the lowest mean % DC in total phytoplankton was 16%q-6% at our sample site, which is in a subtropical area with high water temperatures, full solar radiation, and rich nutrients. This indicates that phytoplankton cell death is a process that cannot be ignored. In summary, phytoplankton cell death is important in understanding the dynamics of phytoplankton communities and the fimctioning of subtropical ecosystems.

关键词： cell death cell digestion assay phytoplankton Xiamen Bay

Rice sl-MH-1 mutant induces cell death and confers blast resistance via the synergistic roles of signaling systems

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The Crop Journal 2022年第6期10卷 1755-1766页

作者： Dagang Tian Yan Lin Shengping Li Yiyang Cao Gang Li Xinrui Guo Ziqiang Chen Zaijie Chen Feng Wang Zonghua Wang Fujian Key Laboratory of Genetic Engineering for Agriculture Biotechnology Research InstituteFujian Academy of Agricultural SciencesFuzhou 350003FujianChina State Key Laboratory of Ecological Control of Fujian-Taiwan Crop Pests Key Laboratory of Ministry of Education for GeneticsBreeding and Multiple Utilization of CropsPlant Immunity CenterFujian Agriculture and Forestry UniversityFuzhou 350003FujianChina

Serotonin is ubiquitous across all forms of life and functions in responses to biotic and abiotic *** rice,the conversion of tryptamine to serotonin is catalyzed by Sekiguchi lesion(SL).Previous studies have identified an sl mutation(a null mutation of SL)in several rice varieties and confirmed its increase of resistance and cell ***,a systematic understanding of the reprogrammed cellular processes causing cell death and resistance is *** performed a multi-omics analysis to clarify the fundamental mechanisms at the protein,gene transcript,and metabolite *** found that cell death and Magnaporthe oryzae(***)infection of the sl-MH-1 mutant activated plant hormone signal transduction involving salicylic acid(SA),jasmonic acid(JA),and abscisic acid(ABA)in multiple regulatory *** characterized the dynamic changes of several key hormone levels during disease progression and under the cell death conditions and showed that SA and JA positively regulated rice cell death and disease ***-overexpressing lines confirmed that the sl-MH-1 mutant positively regulated rice resistance to *** studies shed light on cell death and facilitate further mechanistic dissection of programmed cell death in rice.

关键词： Sekiguchi lesion cell death Phytohormone M.oryzae Rice

The leaf extract of crofton weed(Eupatorium adenophorum)inhibits primary root growth by inducing cell death in maize root border cells

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Plant Diversity 2020年第3期42卷 174-180页

作者： Jinhu Ma Xinxin Feng Xiaohuan Yang Yongheng Cao Weifeng Zhao Liangliang Sun College of Enginemng Shanxi Agricultural UniversityTaigu030807China College of Horticulture Shanxi Agricultural UniversityTaigu030801China College of Agriculture Shanxi Agricultural UniversityTaigu030801China Faculty of Tropical Crops Yunnan Agricultural UniversityPuer665000China

The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely *** this study,we investigated the effects of the leaf extract of crofton weed(LECW) on primary root(PR) growth in maize *** with LECW markedly inhibited seed germination and seedling growth in a dose-dependent *** analysis indicated that the LECW induced reactive oxygen species(ROS) accumulation in root tips,thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips,finally leading to cell death in root border cells(RBCs) and PR growth *** LECW also inhibited pectin methyl esterase(PME) activity,thereby decreasing the RBC *** together,our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in *** present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants.

关键词： Leaf extract of crofton weed Maize cell death Pectin methyl esterase Root border cell

AMPD3 is involved in anthrax LeTx-induced macrophage cell death

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Protein & cell 2011年第7期2卷 564-572页

作者： Sangun Lee Yanhai Wang Sung Ouk Kim Jiahuai Han Department of Immunology and Microbial Science The Scripps Research Institute10550 North Torrey Pine RoadLa JollaCA 92037USA The State Key Laboratory of cellular Stress Biology School of Life SciencesXiamen UniversityXiamen 361005China Current address Department of Biomedical Sciences Tufts UniversityCummings School of Veterinary MedicineNorth GraftonMassachusetts 01536USA Current address Department of Microbiology and Immunology Siebens-Drake Research InstituteUniversity of Western OntarioLondonOntarioCanada

The responses of macrophages to Bacillus anthracis infection are important for the survival of the host,since macrophages are required for the germination of *** spores in lymph nodes,and macrophage death exacerbates anthrax lethal toxin(LeTx)-induced organ *** elucidate the mechanism of macrophage cell death induced by LeTx,we performed a genetic screen to search for genes associated with LeTx-induced macrophage cell ***264.7 cells,a macrophage-like cell line sensitive to LeTx-induced death,were randomly mutated and LeTx-resistant mutant clones were *** deaminase 3(AMPD3),an enzyme that converts AMP to IMP,was identified to be mutated in one of the resistant *** requirement of AMPD3 in LeTxinduced cell death of RAW 264.7 cells was confirmed by the restoration of LeTx sensitivity with ectopic reconstitution of AMPD3 ***3 deficiency does not affect LeTx entering cells and the cleavage of mitogen-activated protein kinase kinase(MKK)by lethal factor inside cells,but does impair an unknown downstream event that is linked to cell *** data provides new information regarding LeTx-induced macrophage death and suggests that there is a key regulatory site downstream of or parallel to MKK cleavage that controls the cell death in LeTx-treated macrophages.

关键词： AMP deaminase 3 anthrax lethal toxin macrophage cell death

ANNEXIN 8 negatively regulates RPW8.1-mediated cell death and disease resistance in Arabidopsis

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Journal of Integrative Plant Biology 2021年第2期63卷 378-392页

作者： Zhi-Xue Zhao Yong-Ju Xu Yang Lei Qin Li Ji-Qun Zhao Yan Li Jing Fan Shunyuan Xiao Wen-Ming Wang Rice Research Institute and Key Laboratory for Major Crop Diseases Sichuan Agricultural UniversityChengdu 611130China Institute for Bioscience and Biotechnology Research&Department of Plant Science and Landscape Architecture University of MarylandRockvilleMaryland 20850USA

Study on the regulation of broad-spectrum resistance is an active area in plant biology. RESISTANCE TO POWDERY MILDEW 8.1(RPW8.1)is one of a few broad-spectrum resistance genes triggering the hypersensitive response(HR) to restrict multiple pathogenic infections. To address the question how RPW8.1 signaling is regulated,we performed a genetic screen and tried to identify mutations enhancing RPW8.1-mediated ***, we provided evidence to connect an annexin protein with RPW8.1-mediated resistance in Arabidopsis against powdery mildew. We isolated and characterized Arabidopsis b7-6 mutant.A point mutation in b7-6 at the At5 g12380 locus resulted in an amino acid substitution in ANNEXIN8(AtANN8). Loss-of-function or RNA-silencing of AtANN8 led to enhanced expression of RPW8.1,RPW8.1-dependent necrotic lesions in leaves, and defense against powdery mildew. Conversely,over-expression of AtANN8 compromised RPW8.1-mediated disease resistance and cell death. Interestingly, the mutation in AtANN8 enhanced RPW8.1-triggered H2O2. In addition, mutation in AtANN8 led to hypersensitivity to salt stress. Together, our data indicate that AtANN8 is involved in multiple stress signaling pathways and negatively regulates RPW8.1-mediated resistance against powdery mildew and cell death, thus linking ANNEXIN’s function with plant immunity.

关键词： AtANN8 cell death disease resistance H2O2 hypersensitive response powdery mildew RPW8.1

Relationship Between Polyamines Metabolism and cell death in Roots of Malus hupehensis Rehd. Under Cadmium Stress

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Journal of Integrative Agriculture 2012年第7期11卷 1129-1136页

作者： JIANG Qian-qian YANG Hong-qiang SUN Xiao-li LI Qiang RAN Kun ZHANG Xin-rong College of Horticulture Science and Engineering Shandong Agricultural University Tai'an 2 71018 P.R. China State Key Laboratory of Crop Biology Shandong Agricultural University Tai'an 271018 P.R.China

The free putrescine （Put） content, the hydrogen peroxide （H202） content and the polyamine oxidase （PAO） activity in roots of Malus hupehensis Rehd. var. pinyiensis Jiang （PYTC） were significantly increased, and reached its peak at 1, 2 and 6 h, respectively, under cadmium treatment. The free spermine （Spin） and spermidine （Spd） contents were dramatically decreased, and reached the minimum value at 4-6 h, then remained relatively stable. The change in total free polyammes （PAs） content was consistent with that of free Put. The number of root dead cells was gradually increased after treatment for 24 h, and the typical characteristics of programmed cell death （PCD） were displayed at 48 h. Throughout the Cd treatment process, changes in PAs metabolism appeared to be prior to cell death increase, and the H2O2 content was always maintained at a high level. These results indicated that polyamines could initiate cell death by generating H2O2 in roots of Malus hupehensis Rehd. under CdSO4 stress.

关键词： Malus hupehensis Rehd. cadmium cell death polyamines metabolism

The Phytophthora nucleolar effector Pi23226 targets host ribosome biogenesis to induce necrotrophic cell death

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Plant Communications 2023年第5期4卷 263-276页

作者： Soeui Lee Jaehwan Kim Myung-Shin Kim Cheol Woo Min Sun Tae Kim Sang-Bong Choi Joo Hyun Lee Doil Choi Plant Immunity Research Center Seoul National UniversitySeoul 08826Republic of Korea Plant Genomics and Breeding Institute Department of AgricultureForestry and BioresourcesCollege of Agriculture and Life ScienceSeoul National UniversitySeoul 08826Republic of Korea Interdisciplinary Programs in Agricultural Genomics College of Agriculture and Life ScienceSeoul National UniversitySeoul 08826Republic of Korea Department of Plant Bioscience Life and Industry Convergence Research InstitutePusan National UniversityMiryang 50463Republic of Korea Division of Bioscience and Bioinformatics Myongji UniversityYongin 449-728Republic of Korea

Pathogen effectors target diverse subcellular organelles to manipulate the plant immune *** the nucleolus has emerged as a stress marker and several effectors are localized in the nucleolus,the roles of nucleolar-targeted effectors remain *** this study,we showed that Phytophthora infestans infection of Nicotiana benthamiana results in nucleolar inflation during the transition from the biotrophic to the necrotrophic *** *** effectors were localized in the nucleolus:Pi23226 induced cell death in *** and nucleolar inflation similar to that observed in the necrotrophic stage of infection,whereas its homolog Pi23015 and a deletion mutant(Pi23226DC)did not induce cell death or affect nucleolar *** immunoprecipitation and individual-nucleotide-resolution UV crosslinking and immunoprecipitation sequencing analysis indicated that Pi23226 bound to the 30 end of 25S rRNA precursors,resulting in accumulation of unprocessed 27S *** nucleolar stress marker NAC082 was strongly upregulated under Pi23226-expressing ***23226 subsequently inhibited global protein translation in host cells by interacting with ***23226 enhanced *** pathogenicity,indicating that Pi23226-induced ribosome malfunction and cell death were beneficial for pathogenesis in the *** results provide evidence for the molecular mechanism underlying RNA-binding effector activity in host ribosome biogenesis and lead to new insights into the nucleolar action of effectors in pathogenesis.

关键词： Phytophthora infestans hemibiotroph nucleolar effector cell death ribosome biogenesis translation inhibition