Cold atmospheric-pressure air plasma treatment of C6 glioma cells:effects of reactive oxygen species in the medium produced by the plasma on cell death

作     者：Yuyang WANG Cheng CHENG Peng GAO Shaopeng LI Jie SHEN Yan LAN Yongqiang YU Paul K CHU 汪宇扬;程诚;高鹏;李少鹏;沈洁;兰彦;余永强;朱剑豪

作者机构：The first Affiliated Hospital of Anhui Medical UniversityHefei 230032People's Republic of China Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031People's Republic of China Department of Physics and Materials ScienceCity University of Hong KongTatChee AvenueKowloonHong KongPeople's Republic of China 

出 版 物：《Plasma Science and Technology》 (等离子体科学和技术（英文版）)

年 卷 期：2017年第19卷第2期

页      面：63-68页

核心收录：

学科分类：1002[医学-临床医学] 07[理学] 070204[理学-等离子体物理] 100214[医学-肿瘤学] 10[医学] 0702[理学-物理学] 

基　　金：jointly supported by the Education and Research Foundation of Anhui Province(KJ2015A327) Science Foundation of Institute of Plasma Physics,Chinese Academy of Sciences No.DSJJ-14-YY02 Hong Kong Research Grants Council(RGC)General Research Funds(GRF)No.CityU 11301215 

主　　题：atmospheric pressure air plasma reactive species cell death 

摘      要：An atmospheric-pressure air plasma is employed to treat C6 glioma cells in vitro. To elucidate on the mechanism causing cell death and role of reactive species （RS） in the medium produced by the plasma, the concentration of the long-lived RS such as hydrogen peroxide, nitrate, and ozone in the plasma-treated liquid （phosphate-buffered saline solution） is measured. When vitamin C is added to the medium as a ROS quencher, the viability of C6 glioma cells after the plasma treatment is different from that without vitamin C. The results demonstrate that reactive oxygen species （ROS） such as H2O2, and O3 constitute the main factors for inactivation of C6 glioma cells and the reactive nitrogen species （RNS） may only play an auxiliary role in cell death.