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A review on cell damage,viability,and functionality during 3D bioprinting

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Military Medical Research 2023年第5期10卷 620-635页

作者： He-Qi Xu Jia-Chen Liu Zheng-Yi Zhang Chang-Xue Xu Department of Industrial Manufacturingand Systems EngineeringTexas Tech UniversityLubbockTX79409USA School of Naval Architecture and Ocean Engineering Huazhong University of Science and TechnologyWuhan430074China

Three-dimensional(3D)bioprinting fabricates 3D functional tissues/organs by accurately depositing the bioink composed of the biological materials and living *** though 3D bioprinting techniques have experienced significant advancement over the past decades,it remains challenging for 3D bioprinting to artificially fabricate functional tissues/organs with high post-printing cell viability and functionality since cells endure various types of stress during the bioprinting ***,cell viability which is affected by several factors including the stress and the environmental factors,such as pH and temperature,is mainly determined by the magnitude and duration of the stress imposed on the cells with poorer cell viability under a higher stress and a longer duration *** maintenance of high cell viability especially for those vulnerable cells,such as stem cells which are more sensitive to multiple stresses,is a key initial step to ensure the functionality of the artificial tissues/*** addition,maintaining the pluripotency of the cells such as proliferation and differentiation abilities is also essential for the 3D-bioprinted tissues/organs to be similar to native tissues/*** review discusses various pathways triggering cell damage and the major factors affecting cell viability during different bioprinting processes,summarizes the studies on cell viabilities and functionalities in different bioprinting processes,and presents several potential approaches to protect cells from injuries to ensure high cell viability and functionality.

关键词： Three-dimensional bioprinting cell damage Shear stress cell viability cell functionality

Low-Dose Gamma Radiation Fields Decrease cell Viability, damage DNA, and Increase the Expression of Hsp70 and p53 Proteins in Human Leukocytes

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World Journal of Nuclear Science and Technology 2023年第4期13卷 55-72页

作者： Jessica Lizbeth Sifuentes Padilla David Alejandro García López Consuelo Letechipia de León Hector Rene Vega-Carrillo Sergio Hugo Sánchez Rodríguez Laboratorio de Biología Celular y Neurobiología Unidad Académica de Ciencias Biológicas Universidad Autónoma de Zacatecas Zacatecas México Laboratorio de Radiobiología Unidad Académica de Estudios Nucleares Universidad Autónoma de Zacatecas Zacatecas México

Ionizing radiations are tools in diagnosis and treatment of diseases. Leukopenia from exposure to ionizing radiation has been reported. Due to their radiosensitivity, leukocytes are a biological model to analyze cell damage. Therefore, cell viability, DNA damage, and Hsp70 and p53 expression in human leukocytes exposed to low-dose gamma radiation fields from a 137Cs source were evaluated. A decrease in cell viability, DNA damage and an increase in the expression of Hsp70 and p53 proportional to the radiation dose received was found, which was 0.2, 0.4, 0.6, 0.8 and 1.0 mGy.

关键词： Leukocytes Gamma Radiation cell damage DNA Hsp70 p53

Vertical nanowires enhanced X-ray radiation damage of cells

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Journal of Materials Science & Technology 2023年第14期145卷 7-13页

作者： Liyuan Zheng Qingxuan Li Shandong Xu Xiaofeng Meng Xinqi Chen Ming Su Department of Chemical Engineering Northeastern UniversityBostonMassachusetts 02115United States Wenzhou Institute of Biomaterials and Engineering Chinese Academy of Sciencesand Wenzhou Medical UniversityWenzhou 325001China College of Sciences Beijing Forestry UniversityBeijing 100083China Department of Mechanical Engineering Northwestern UniversityEvanstonIllinois 60208United States

cell behavior is affected by nanostructured surface,but it remains unknown how ionizing radiation af-fects cells on nanostructured *** paper reports an experimental investigation of X-ray radiation induced damage of cells placed on an array of vertically aligned silicon nanowires.X-ray photoelectrons and secondary electrons produced from nanowire array are measured and compared to those from flat silicon *** cell functions including morphology,viability,adhesion and proliferation have been examined and found to be drastically affected when cells are exposed to X-ray radiation,compared to those sitting on flat substrate and those only exposed to *** enhanced cell damage on nanowires upon X-ray exposure is attributed to nanowire enhanced production of photoelectrons including Auger electrons and secondary electrons,which have high escaping probability from sharp tips of *** escaped photoelectrons ionize water molecules and generate hydroxyl free radicals that can damage DNAs of *** inference of this work is that the contrast in scanning electron microscopy is useful in assessing the effects of nanomaterials for enhanced X-ray radiation therapy.

关键词： Edge enhancement Silicon nanowire array X-ray radiation Photoelectron cell damage

Protocatechuic acid and quercetin attenuate ETEC-caused IPEC-1 cell inflammation and injury associated with inhibition of necroptosis and pyroptosis signaling pathways

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Journal of Animal Science and Biotechnology 2023年第4期14卷 1551-1568页

作者： Kan Xiao Mohan Zhou Qingqing Lv Pengwei He Xu Qin Dan Wang Jiangchao Zhao Yulan Liu Hubei Key Laboratory of Animal Nutrition and Feed Science Wuhan Polytechnic UniversityWuhan 430023People’s Republic of China Department of Animal Science Division of AgricultureUniversity of ArkansasFayettevilleAR 72701USA

Background:Necroptosis and pyroptosis are newly identified forms of programmed cell death,which play a vital role in development of many gastrointestinal *** plant polyphenols have been reported to protect intestinal health,it is still unclear whether there is a beneficial role of plant polyphenols in modulating necroptosis and pyroptosis in intestinal porcine epithelial cell line(IPEC-1)infected with enterotoxigenic Escherichia coli(ETEC)*** research was conducted to explore whether plant polyphenols including protocatechuic acid(PCA)and quercetin(Que),attenuated inflammation and injury of IPEC-1 caused by ETEC K88 through regulating necroptosis and pyroptosis signaling ***:IPEC-1 cells were treated with PCA(40μmol/L)or Que(10μmol/L)in the presence or absence of ETEC ***:PCA and Que decreased ETEC K88 adhesion and endotoxin level(Pcell *** and Que increased cell number(Pcell supernatant after ETEC *** and Que improved transepithelial electrical resistance(TEER)(Pcell necrosis ratio(P<0.05).PCA and Que reduced mRNA abundance and concentration of tumor necrosis factor-α(TNF-α),interleukin(IL)-6 and IL-8(P<0.001),and down-regulated gene expression of toll-like receptors 4(TLR4)and its downstream signals(P<0.001)after ETEC *** and Que down-regulated protein abundance of total receptor interacting protein kinase 1(t-RIP1),phosphorylated-RIP1(p-RIP1),p-RIP1/t-RIP1,t-RIP3,p-RIP3,mixed lineage kinase domain-like protein(MLKL),p-MLKL,dynamin-related protein 1(DRP1),phosphoglycerate mutase 5(PGAM5)and high mobility group box 1(HMGB1)(P<0.05)after ETEC infection.M

关键词： cell damage ETEC K88 Intestinal inflammation Necroptosis Protocatechuic acid Pyroptosis Quercetin

Melatonin restores zinc levels,activates the Keap1/Nrf2 pathway,and modulates endoplasmic reticular stress and HSP in rats with chronic hepatotoxicity

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World Journal of Gastrointestinal Pharmacology and Therapeutics 2022年第2期13卷 11-22页

作者： Silvia Bona Sabrina Alves Fernandes Andrea C Janz Moreira Graziella Rodrigues Elizângela G Schemitt Fabio Cangeri Di Naso Cláudio A Marroni Norma P Marroni Medical Sciences Program Universidade Federal do Rio Grande do SulPorto Alegre 90035-903Rio Grande do SulBrazil Posgraduate Program in Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90040-001Rio Grande do SulBrazil Biological Sciences Program Universidade Federal do Rio Grande do SulPorto Alegre 90050-170Rio Grande do SulBrazil Postgraduate Program in Pneumological Sciences Universidade Federal do Rio Grande do SulPorto Alegre 90000-000Rio Grande do SulBrazil

BACKGROUND Melatonin(MLT)is a potent antioxidant molecule that is shown to have a beneficial effect in various pathological situations,due to its action against free *** To evaluate the effect of MLT on carbon tetrachloride(CCl_(4))induced liver injury in rats in terms of oxidative stress,reticular stress,and cell *** Twenty male Wistar rats(230-250 g)were divided into four groups:Control rats,rats treated with MLT alone,rats treated with CCl_(4)alone,and rats treated with CCl_(4)plus ***_(4)was administered as follows:Ten doses every 5 d,ten every 4 d,and seven every 3 *** was administered intraperitoneally at a dose of 20 mg/kg from the 10th wk to the end of the experiment(16th wk).RESULTS MLT was able to reduce the release of liver enzymes in the bloodstream and to decrease oxidative stress in CCl_(4)treated rats by decreasing the level of thiobarbituric acid reactive substances and increasing superoxide dismutase activity,with a lower reduction in serum zinc levels,guaranteeing a reduction in liver damage;additionally,it increased the expression of nuclear factor(erythroid-derived 2)-like 2 and decreased the expression of Kelch-like ECH-associated protein *** also decreased the expression of the proteins associated with endoplasmic reticulum stress,i.e.,glucose-regulated protein 78 and activating transcription factor 6,as well as of heat shock factor 1 and heat shock protein *** MLT has a hepatoprotective effect in an experimental model of CCl_(4)-induced liver injury,since it reduces oxidative stress,restores zinc levels,and modulates endoplasmic reticulum stress.

关键词： Liver injury cell damage Antioxidant Melatonin Carbon tetrachloride

Protective Effect of Buyang Huanwu Decoction on Cerebral Ischemia/Reperfusion Injury by Inhibiting Autophagy Based on AMPK/UIK1Signaling Pathway

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Journal of Integrative Medicine（双语） 2022年第2期11卷 9-28页

作者： Ruijuan Zhang Yanmeng Zhao Xiujuan Ma Jiabei Ye Yudong Shan Wentao Yu Xiaohong Zhou Weijuan Gao Hebei University of Chinese Medicine No.3Xingyuan RoadLuquan DistrictShijiazhuangHebei050200China

The aim of the paper is to explore whether AMPK/U1K1 signaling pathway is involved in regulating autophagy and alleviating cerebral ischemia/reperfusion(I/R)*** this basis,study the regulatory effect of Buyang Huanwu Decoction(BHD)on AMPK/U1K1 signaling pathway,aiming to clarify the molecular mechanism of BHD in alleviating cerebral I/R injury and the protective effect of BHD containing serum(BHDS)on OGD/R *** research group adopted the following methods:Neurons cultured for 7 days in vitro were randomly divided into normal control group,model group(OGD/R),AMPK activator(AICAR)group(3 mmol/L),Buyang Huanwu Decoction medicated serum(BHDS)group(3%),Buyang Huanwu Decoction medicated serum+AMPK activator(BHDS+AICAR)*** protein expressions of p-AMPK,AMPK,p-UlKl,U1K1,Beclin-1 and LC3B in neurons were detected by Western blot,the fluorescence intensity and apoptosis rate of neuron autophagosome CYTO-ID staining were detected by flow cytometry,the fluorescence intensity and morphological changes of neuron LC3B were detected by immunofluorescence double labeling method,the morphological changes of neurons were observed by inverted microscope,and the survival rate of neurons was detected by CCK-8 *** OGD/R model and the optimal concentration of *** neurons in the model group were subjected to hypoxia and glucose deprivation for 1 h,2 h,3 h,4 h and reoxygenation and glucose deprivation for 24 *** survival rate of neurons and the release rate of 1DH to determine whether the model was successfully *** the results show that BHDS group,the survival rate of OGD/R neurons was significantly increased,and the apoptosis rate was significantly *** them,3%BHDS intervention at the same time of hypoxia glucose deprivation for 3 h and reoxygenation glucose for 24 h was the best *** BHDS group,the levels of p-AMPK/AMPK,p-UlKl/UlKl,beclin-1,CYTO-ID and LC3B fluorescence intensity,and the transformation from LC3B-I to LC3B-I

关键词： BHDS Drug containing serum Traditional Chinese medicine Signal pathway cell damage