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Identification of stable internal control genes for accurate normalization of real-time quantitative PCR data in testicular tissue from two breeds of cattle
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Asian pacific Journal of Reproduction 2020年 第5期9卷 247-255页
作者: Pradeep Nag Ankur Sharma Elango Kamaraj Arumugam Kumaresan Tirtha Kumar Datta Ayyasamy Manimaran Nilendu Paul Sakthivel Jeyakumar Kerekoppa P.Ramesha Theriogenology Laboratory Veterinary Gynaecologyand ObstetricsSouthern Regional Station of ICAR-National Dairy Research InstituteBengaluru–560030 KarnatakaIndia Animal Genomics Laboratory ICAR-National Dairy Research InstituteKarnal–132001 HaryanaIndia Southern Regional Station of ICAR-National Dairy Research Institute Bengaluru–560030 KarnatakaIndia
Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and... 详细信息
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