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色谱 2017年第10期35卷 1042-1047页

作者： CHEUK Man-Yung LO Yun-Chuen POON Wing-Tat Department of Pathology Pamela Youde Nethersole Eastern Hospital

The measurement of urine catecholamine and metanephrine concentrations is important for biochemical screening and diagnosis of pheochromocytoma.The goal of this work was to develop a simple liquid chromatography-tandem mass spectrometry(LC-MS/MS)method for determining catecholamines and metanephrines in urine to replace an existing liquid chromatographic method using electrochemical detection.Urine samples were prepared using Oasis weak-cation-exchange cartridges.The eluate was analyzed on an Agilent ZORBAX Eclipse Plus Phenyl-Hexyl column in 3 min.Adrenaline,noradrenaline,dopamine,metanephrine,normetanephrine,and their deuterated internal standards were monitored in positive electrospray ionization mode by multiple reaction monitoring(MRM).No evidence of ion suppression was observed.The assay was linear up to 5μmol/L for adrenaline,5μmol/L for noradrenaline,6.1μmol/L for dopamine,5.6μmol/L for metanephrine,and 34.6μmol/L for normetanephrine,with lower limits of quantification of 5,5,12,6 and 7nmol/L,respectively.The intra-day and inter-day precisions for all analytes ranged from 0.59%to 4.64%and1.98%to 4.80%,respectively.External quality assurance samples were assayed and showed excellent agreement with the target values.This simple method provides an improved assay for determining urine catecholamines and metanephrines.

关键词： liquid chromatography-tandem mass spectrometry (LC-MS/MS) metanephrines catecholamines pheochromocytoma urine

Determination of catecholamines by ion chromatography coupled to acidic potassium permanganate chemiluminescence detection

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Chinese Chemical Letters 2012年第7期23卷 839-842页

作者： Hong Wei Wu Mei Lan Chen Dan Shou Yan Zhu Department of Chemistry Xixi CampusZhejiang UniversityHangzhou 310028China Department of Chemistry Xinxiang Medical UniversityXinxiang 453003China College of Biology and Environment Engineering Zhejiang Shuren UniversityHangzhou 310015China ZheJiang Academy of Traditional Chinese Medicine Hangzhou 310007China

A simple,fast,sensitive,highly selective and eco-friendly analytical method for the determination of catecholamines in human urine by ion chromatography（IC） with chemiluminescence（CL） detection was described in this paper.Using 12 mmol/L H_2SO_4 without any organic additive as eluent,three catecholamines including epinephrine（EP）,norepinephrine（NE） and dopamine（DA） were well separated on a cation-exchange column.The CL detection was based on the reaction of analytes with acidic potassium permanganate in the presence of formaldehyde as an enhancer.The absence of methanol and acetonitrile in eluent made the proposed method more sensitive and eco-friendly.Under the optimal conditions,the linear range of the proposed method was in the range of 0.02-0.5μg/mL.The limit of detection（LOD） was in the range of 0.6 and 5.1μg/L.The relative standard deviations （RSD） for 0.1μg/mL mixed standard solution were in the range of 0.8-1.9%（n = 11）.The method has been applied to the determination of catecholamines in human urine successfully.Excellent spiked recoveries were achieved for catecholamines ranged from 91.2%to 112.7%.

关键词： catecholamines Ion chromatography Chemiluminescence Urine

Capillary electrophoresis with direct chemiluminescence detection for the analysis of catecholamines in human urine

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Chinese Chemical Letters 2007年第4期18卷 452-454页

作者： Cheng Quan Wang Hui Wang Yan Ming Liu College of Chemistry and Chemical Engineering Xinyang Normal University Xinyang 464000 China

A rapid and sensitive method for the analysis of three catecholamines by capillary electrophoresis （CE） with direct chemiluminescence （CL） detection is described. The detection limits （S/N= 3） were 1.3 × 10^-8 g/mL for isoprenaline, 1.0 × 10^-8g/mL for epinephrine and 2.8 × 10^-8 g/mL for dopamine. The proposed method was successfully applied to the analysis of catecholamines in urine samples of cigarette smokers and nonsmokers. The results showed that there is a close relation between the release of dopamine in human body fluids and cigarette smoking/nonsmoking.

关键词： Capillary electrophoresis catecholamines Chemiluminescence detection Urine

Crosstalk between catecholamines and erythropoiesis

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Frontiers in Biology 2017年第2期12卷 103-115页

作者： Fakhredin Saba Najmaldin Saki Elahe Khodadi Masoud Soleimani Department of Laboratory Science Kermanshah University of Medical Sciences Kermanshah Iran Department of Hematology Faculty of Medical Sciences Tarbiat Modares University Tehran Iran Health Research Institute Research Center of Thalassemia & Hemoglobinopathy Ahvaz Jundishapur University of Medical Sciences Ahvaz Iran

BACKGROUND： Erythropoiesis is regulated by a range of intrinsic and extrinsic factors, including different cytokines. Recently, the role of catecholamines has been highlighted in the development of erythroid cell lineages. OBJECTIVE： This study focuses on the biological links interconnecting erythroid development and the sympathetic nervous system. The emerging evidence that underscores the role of catecholamines in the regulation of erythropoietin and other erythropoiesis cytokines are thoroughly reviewed, in addition to elements such as iron and the leptin hormone that are involved in erythropoiesis. METHODS： Relevant English-language studies were identified and retrieved from the PubMed search engine （1981-2017） using the following keywords：＂Erythropoiesis＂, ＂catecholamines＂, ＂Nervous system＂, and ＂Cytokines.＂ RESULTS： Chronic social stress alters and suppresses erythroid development. However, the physiological release of catecholamines is an additional stimulator of erythropoiesis in the setting of anemia. Therefore, the severity and timing of catecholamine secretion might distinctly regulate erythroid homeostasis. CONCLUSION： Understanding the relationship of catecholamines with different elements of the erythroid islands will be essential to find the tightly regulated production of red blood cells （RBCs） in both chronic and physiological catecholamine activation.

关键词： erythropoiesis cytokines catecholamines chronic social stress nervous system

Modified monolithic silica capillary for preconcentration of catecholamines

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Journal of Pharmaceutical Analysis 2009年第3期21卷 160-162页

作者： Tusyoshi Komazu Department of Chemistry Tokyo Metropolitan University

Preconcentration of catecholamines by the modified monolithic silica in the capillary was investigated in this study. In order to achieve a microchip-based method for determining catecholamines in the saliva,the monolithic silica was fabricated in the capillary and the monolithic silica was chemically modified by on-column reaction with phenylboronate. Different modified methods were compared. The concentration conditions were optimized. This study indicates the applicability of the modified monolithic silica capillary when it was used to concentrate catecholamines.

关键词： catecholamines monolithic silica preconcentration

Understanding the impact of stress on teleostean reproduction

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Aquaculture and Fisheries 2022年第5期7卷 553-561页

作者： Raju Murugananthkumar Cheni-Chery Sudhakumari Department of Animal Biology School of Life SciencesUniversity of HyderabadP.O.Central UniversityHyderabad500046India Section on Molecular endocrinology Division of Developmental BiologyEunice Kennedy Shriver National Institute of Child Health and Human Development(NICHD)National Institutes of Health(NIH)BethesdaMD20892-4510USA.

Fishes exert stress response in a various ways depending on the type of the stressor.The stress responses are activated through a cascade mechanism stimulated by the stressor which involves the hypothalamus-hypophyseal-interrenal(HHI)axis,catecholamines(CA),and gonadotropins.Adaptive stress responses may positively impact the fish survival and reproduction,while continuous or prolonged stress causes adverse effects on the fish reproduction.Corticotropin-releasing factor and adrenocorticotropic hormone are the principal hormones responsible for producing corticosteroids through the HHI axis.Cortisol acts differentially on the stress response as it helps at the early developmental stage;conversely,it impairs the gonadal function.CA have a critical role in maintaining body homeostasis and intermediary metabolism,and they also have a predominant role in reproductive function.Besides hormones,few genetic and epigenetic factors have been identified to understand the molecular responses to stress however,genome-wide associated studies will be initiated to investigate a complete picture of the stress mechanism.Further,recent evidence suggests a growing concern in determining the correlation between the stress hormone level and its associated gene function.Hence,this review highlights the regulation of stress responses in different axes,genetic and epigenetic factors related to stress,and the integration of recent technologies and novel hypotheses to unravel the stress response mechanism in fish reproduction.

关键词： Cortisol Interrenal axis Steroidogenesis Gonadotropins Chromaffin cells catecholamines

Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/β_(2)-AR/MACC1 axis

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Cancer Communications 2021年第10期41卷 1049-1070页

作者： Changqie Pan Jianhua Wu Siting Zheng Huiying Sun Yisheng Fang Zhenhua Huang Min Shi Li Liang Jianping Bin Yulin Liao Jinzhang Chen Wangjun Liao Department of Oncology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515P.R.China Department of Pathology Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515P.R.China Department of Cardiology Nanfang HospitalSout4hern Medical UniversityGuangzhouGuangdong 510515P.R.China

Background:Depression is a common,easily ignored,accompanied disease of gastric cancer(GC)patients and is often observed with elevated plasma catecholamine levels.Depression frequently promotes GC progression and leads to poor clinical outcomes;however,the molecular mechanisms underlying depression-induced GC progression remain poorly understood.We aimed to study the effects of depression on GC progression and explore possible mechanisms mediating the action of depression-associated catecholamines on GC.Methods:Depression states of GC patients were graded using the Patient Health Questionnaire-9,and plasma catecholamine levels were examined by high performance liquid chromatography coupled with tandem mass spectrometry.Migrative and invasive GC cells were examined using transwell assays,and metastatic GC niches were imaged using bioluminescence technology in a depression mouse model established with chronic unpredictable mild stress.Mouse depression-like behaviors were assessed through sucrose preference,forced swimming,and tail suspension tests.Characteristics of the neuroendocrine phenotype were observed via RT-PCR,Western blotting,flow cytometry,and transmission electron microscopy.Results:Fifty-one GC patients(age:53.61±1.79 years;cancer duration:3.71±0.33 months;depression duration:2.37±0.38 months;male-to-female ratio:1.55:1)were enrolled in the study.Depression grade was significantly higher in GC patients showing higher plasma levels of catecholamines(epinephrine:P=0.018;noradrenaline:P=0.009),higher oncogene metastasis-associated in colon cancer-1(MACC1)level(P=0.018),and metastasis(P<0.001).Further,depression-associated catecholamine specifically bound to the beta-2 adrenergic receptor(β_(2)-AR)and upregulated MACC1 expression,and thus promoting neuroendocrine phenotypic transformation through direct binding betweenMACC1 and synaptophysin.Eventually,the neuroendocrine phenotypic transformation accelerated GC invasion in vitro and metastasis in vivo.However,β_(2)-AR antagonist ICI-118,551 or MACC1 silenc

关键词： catecholamines depression gastric cancer MACC1 neuroendocrine phenotype psychological stress tumor metastasis

Locus coeruleus-norepinephrine: basic functions and insights into Parkinson’s disease

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Neural Regeneration Research 2020年第6期15卷 1006-1013页

作者： Bilal Abdul Bari Varun Chokshi Katharina Schmidt The Solomon H.Snyder Department of Neuroscience Brain Science InstituteKavli Neuroscience Discovery InstituteThe Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Physiology The Johns Hopkins University School of MedicineBaltimoreMDUSA

The locus coeruleus is a pontine nucleus that produces much of the brain's norepinephrine.Despite its small size,the locus coeruleus is critical for a myriad of functions and is involved in many neurodegenerative and neuropsychiatric disorders.In this review,we discuss the physiology and anatomy of the locus coeruleus system and focus on norepinephrine's role in synaptic plasticity.We highlight Parkinson's disease as a disorder with motor and neuropsychiatric symptoms that may be understood as aberrations in the normal functions of locus coeruleus.

关键词： catecholamines copper neurodegenerative diseases neuromodulation neuronal circuits neuropsychiatric symptoms noradrenaline synaptic plasticity

Regulation of natural killer cell activity by glucocorticoids,serotonin,dopamine,and epinephrine

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Cellular & Molecular Immunology 2020年第7期17卷 705-711页

作者： Silvia Capellino Maren Claus Carsten Watzl Department for Immunology Leibniz Research Centre for Working Environment and Human Factors(IfADo)at TU DortmundDortmundGermany

The immune system and the nervous system are highly complex organs composed of various different cells that must interact with each other for proper function of the system.This communication can be mediated by soluble factors.The factors released by the nervous system(neurotransmitters)differ from those released by the immune system(cytokines).Nevertheless,the nervous and immune systems can influence each other’s activity because immune cells express neurotransmitter receptors,and neurons express cytokine receptors.Moreover,immune cells can synthesize and release neurotransmitters themselves,thus using neurotransmittermediated pathways via autocrine and paracrine mechanisms.Natural killer(NK)cells are innate lymphocytes that are important for early and effective immune reactions against infections and cancer.Many studies have shown the strong influence of stress and the nervous system on NK cell activity.This phenomenon may be one reason why chronic stress leads to a higher incidence of infections and cancer.Here,we review the effects of neuroendocrine factors on the different activities of NK cells.Understanding the effects of neuroendocrine factors on NK cell activities during physiological and pathophysiological conditions may result in novel therapeutic strategies to enhance NK cell functions against tumors.

关键词： Natural Killer Cells catecholamines Glucocorticoids Neurotransmitters

Differential Effects of d, l-Sotalol and d-Sotalol on Isoproterenol-Increased Delayed Rectifier Outward Potassium Current in Guinea Pig Single Ventricular Myocytes

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Journal of Huazhong University of Science and Technology(Medical Sciences) 1998年第1期18卷 13-17页

作者：姚晓宙陆再英赵华月 Department of Cardiology University Hospital Heidelberg University Germany Department of Cardiology Tongji Hospital Tongji Medical University Wuhan

The aim of this study was to compare the effects of d, l-Sotalol and dSotalol on the delayed rectifier K+ outward current in the presence of isoproterenol at different concentrations. Time-dependent delayed rectifier K+ outward currents were measured in isolated guinea pig single myocytes using the whole-cell configuration of the patch-clamp technique. Currents were measured in response to 300 ms depolarizing pulses from a holding potential of -40 mV in three experimental protocols [control, isoproterenol (10^(9)mol/L - 10^(-6) mol/L ), and isoproterenol (10^(-9)mol/L - 10^(-6)mol/L ) plus either d, l-Sotalol (10^(-4) mol/L) or d-Sotalol (10^(-4) mol/L)]. IK tail currents were measured upon repolarization to -40 mV. It was found that Ik was significantly amplified in the presence. of isoproterenol (10^(-9) mol/L- 10^(-6) mol/L) plus d-Sotalol. At 10-8 mol/L isoproterenol, Ik was increased by 92. 7%±17. 1 % (P<0. 05) and 54. 3 %±13. 4 % after d-Sotalol addition (P<0. 05). In contrast, d, l-Sotalol completely conteracted the increase of iK by isoproterenol (<10^(-8) mol/L), and compared to control, Ic was decreased by 35. 6 % ±8. 1% at 10^(-8) mol/L isoproterenol plus d, l-Sotalol (P<0. 05). It is concluded that the β-adrenergic blocking property of d, l-Sotalol but not that of dSotalol maintains the delayed rectifier K+ outward current blockade in the presence of isoproterenol in guinea pig myocytes. This might contribute to a superior antiarrhythmic efficacy as compared to d-Sotalol.

关键词： potassium channel delayed rectifier current antiarrhythmia agents cardiomyocytes catecholamines