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Comparison of dynamic changes in endogenous hormones and sugars between abnormal and normal castanea mollissima

Progress in Natural Science:Materials International 2008年第6期18卷 685-690页

作者： Tao Liua,b,Yunqian Hua,*,Xiaoxian Li a aKunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China bGraduate University of the Chinese Academy of Sciences,Beijing 100049,China Kunming Institute of Botany Chinese Academy of Sciences Kunming 650204 China Graduate University of the Chinese Academy of Sciences Beijing 100049 China

To elucidate the possible functions of endogenous hormones in the flowering of chestnut,concentrations of four endogenous hor-mones[indole-3-acetic acid(IAA),gibberellic acid(GA),abscisic acid(ABA),zeatin riboside(ZR))and the soluble sugars content were measured in both normal and developmentally abnormal Chinese chestnut(castanea mollissima)during flowering and fruiting *** results showed that the contents of ZR,ABA,and GA exhibited a significant dierent pattern in normal trees from that in abnormal trees,while the contents of IAA and soluble sugars showed a similar change pattern between *** results suggest that quantitative changes in endogenous hormones may correspond to dierent flowering and fruiting mechanisms.

关键词： castanea mollissima Endogenous hormones Soluble sugars

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Fingerprinting 146 Chinese chestnut(castanea mollissima Blume)accessions and selecting a core collection using SSR markers

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Journal of Integrative Agriculture 2021年第5期20卷 1277-1286页

作者： NIE Xing-hua WANG Ze-hua LIU Ning-wei SONG Li YAN Bo-qian XING Yu ZHANG Qing FANG Ke-feng ZHAO Yong-lian CHEN Xin WANG Guang-peng QIN Ling CAO Qing-qin Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206P.R.China College of Plant Science and Technology Beijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China College of Landscape Architecture Beijing University of AgricultureBeijing 102206P.R.China Chestnut Technology Experiment and Extension Station of Huairou District Beijing 102206P.R.China Shandong Institute of Pomology Shandong Academy of Agricultural SciencesTai’an 271000P.R.China Changli Institute of Pomology Hebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China

Chinese chestnut is an important nut tree around the *** the types of Chinese chestnut resources are abundant,resource utilization and protection of chestnut accessions are still very ***,we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut *** analyses showed that these markers from the tested accessions are highly polymorphic,with an average allele number(N_(a))and polymorphic information content(PIC)of 8.100 and 0.622 per locus,*** these strongly distinguishing markers,we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different *** exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups:group I(North China(NC)and Northwest China(NWC)cultivar groups),group II(middle and lower reaches of the Yangtze River(MLY)cultivar group)and group III(Southeast China(SEC)and Southwest China(SWC)cultivar groups).Finally,we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut *** study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups,which can serve as the basis for efficient breeding in the future.

关键词： castanea mollissima SSR markers fingerprinting phylogeny core collections

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CTAB-silica Method for DNA Extraction and Purification from castanea mollissima and Ginkgo biloba

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Forestry Studies in China 2003年第3期5卷 10-12页

作者： Shen Yongbao Shi Jisen National Southern Tree Seed Inspection Center Nanjing Forestry UniversityNanjing 210037P.R.China

A new method CTAB-silica for DNA extraction and purification from the leaves and buds of castanea mollissima and Ginkgo biloba was tested. The method is based on the silica-based purification protocol developed by Boom et al. (1990). By modifying the protocol, plant genome DNA could be extracted easily from dormant buds, mature leaves, and other parts of plant. Our results showed that the purified DNA was of high purity and could be analyzed by PCR. Furthermore, this CTAB-silica method took much less time for a successful DNA purification process compared to the traditional methods (CTAB and SDS). By our method, the suitable DNA can be extracted and purified from over 10 plant samples by one person in an hour.

关键词： DNA extraction and purification CTAB-silica method castanea mollissima Ginkgo biloba

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