Fingerprinting 146 Chinese chestnut(Castanea mollissima Blume)accessions and selecting a core collection using SSR markers

作     者：NIE Xing-hua WANG Ze-hua LIU Ning-wei SONG Li YAN Bo-qian XING Yu ZHANG Qing FANG Ke-feng ZHAO Yong-lian CHEN Xin WANG Guang-peng QIN Ling CAO Qing-qin NIE Xing-hua;WANG Ze-hua;LIU Ning-wei;SONG Li;YAN Bo-qian;XING Yu;ZHANG Qing;FANG Ke-feng;ZHAO Yong-lian;CHEN Xin;WANG Guang-peng;QIN Ling;CAO Qing-qin

作者机构：Beijing Advanced Innovation Center for Tree Breeding by Molecular DesignBeijing University of AgricultureBeijing 102206P.R.China College of Plant Science and TechnologyBeijing Key Laboratory for Agricultural Application and New TechniqueBeijing University of AgricultureBeijing 102206P.R.China College of Landscape ArchitectureBeijing University of AgricultureBeijing 102206P.R.China Chestnut Technology Experiment and Extension Station of Huairou DistrictBeijing 102206P.R.China Shandong Institute of PomologyShandong Academy of Agricultural SciencesTai’an 271000P.R.China Changli Institute of PomologyHebei Academy of Agriculture and Forestry SciencesChangli 066600P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2021年第20卷第5期

页      面：1277-1286页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 090201[农学-果树学] 0713[理学-生态学] 

基　　金：the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality,China(IDHT20180509) the National Key Research&Development Program of China(2018YFD1000605) the Opening Project of Beijing Key Laboratory of New Technology in Agricultural Application,China(kf2018024) 

主　　题：Castanea mollissima SSR markers fingerprinting phylogeny core collections 

摘      要：Chinese chestnut is an important nut tree around the *** the types of Chinese chestnut resources are abundant,resource utilization and protection of chestnut accessions are still very ***,we fingerprinted and determined the genetic relationships and core collections of Chinese chestnuts using 18 fluorescently labeled SSR markers generated from 146 chestnut *** analyses showed that these markers from the tested accessions are highly polymorphic,with an average allele number(N_(a))and polymorphic information content(PIC)of 8.100 and 0.622 per locus,*** these strongly distinguishing markers,we successfully constructed unique fingerprints for 146 chestnut accessions and selected seven of the SSR markers as core markers to rapidly distinguish different *** exploration of the genetic relationships among the five cultivar groups indicated that Chinese chestnut accessions are divided into three regional type groups:group I(North China(NC)and Northwest China(NWC)cultivar groups),group II(middle and lower reaches of the Yangtze River(MLY)cultivar group)and group III(Southeast China(SEC)and Southwest China(SWC)cultivar groups).Finally,we selected 45 core collection members which represent the most genetic diversity of Chinese chestnut *** study provides valuable information for identifying chestnut accessions and understanding the phylogenetic relationships among cultivar groups,which can serve as the basis for efficient breeding in the future.