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Chinese Science Bulletin 2002年第12期47卷 1019-1023页

作者： XU Lei CHEN Jianping YE Rong ZHAO Xiuyun YU Shanqing Microbiology Department Fudan UniversityShanghai 200433China Virology Laboratory Zhejiang Academy of Agricultural SciencesHangzhou 200433China

The 5’-terminal (RTn) and 3’-terminal (RTc) halves of the coat protein readthrough domain and the 19 ku cysteine-rich protein of Chinese wheat mosaic virus (CWMV) were amplified by RT-PCR, cloned and expressed in E. coll. Antisera and monoclonal antibodies against these proteins were prepared by immunising these purified proteins to mice. Detection of RTn, RTc and 19 ku proteins in CWMV infected wheat sap and leaf tissue indicated that the RTn and RTc proteins were distributed on the surface of virus particles whereas the 19 ku protein was in the cytoplasm of the infected wheat cells.

关键词： Chinese wheat mosaic virus coat protein readthrough domain cysteine-rich domain prokaryotic expression localisation.

TURNIP MOSAIC VIRUS coat PROTEIN GENE: CLONING AND CONSTRUCTION OF THE PLANT VECTOR

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Science China Chemistry 1992年第12期35卷 1444-1452页

作者：孔令洁方荣祥陈正华莽克强 Institute of Genetics Academia Sinica Beijing 100101 PRC Institute of Microbiology Academia Sinica Beijing 100080 PRC

Complementary DNAs to turnip mosaic virus (a radish Raphanus stativus isolate) genomicRNA were synthesized using oligo (dT) as primer and cloned into the vector λ-ZAP Ⅱ. Afterhybridization with a single-stranded cDNA probe and the sequencing of inserted DNA,positive clones with poly--A tails were obtained. One clone containing 1429-base pair insertwas sequenced. The coat protein gene was identified based on the molecular weight of theTuMV coat protein and the consensus sequences of the polyprotein processing sites ofpotyviruses. The 5’ end of the coat protein gene was modified by PCR to introduce aninitiation codon, ATG, and two restriction enzyme sites. The gene was then manipulatedinto a binary vector pBIN437 which was derived from pBI121, and the plant expressionvector is being used to transform Brassica napus.

关键词： turnip mosaic virus coat protein gene plant expression vector

Foreign peptides displayed on the major coat protein of filamentous bacteriophage

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Chinese Science Bulletin 1998年第14期43卷 1158-1162页

作者： WANG Li Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China Institute of Genetics and Cytology Northeast Normal University Changchun China

The genome of filamentous bacteriophage can be engineered to display foreign peptides on the surface of the major coat protein. This display system offers an effective approach to researching the immunological recognition of protein. Studies show that this system has several advantages: 1) the specificity of the immune response; 2) the ability to recruit helper T cells; 3) needs no external adjuvants; 4) the structural mimicry of peptide epitopes. These suggest that this technology could be developed into a simple and inexpensive means to produce new biological reagents and vaccines. The development of this technology and the main property of filamentous bacteriophage are introduced in this paper. Some novel results about foreign peptides display using the main coat protein of filamentous bacteriophage are also summarized.

关键词： filamentous bacteriophage major coat protein epitope.

The role of MC1R gene in buffalo coat color

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Science China(Life Sciences) 2010年第2期53卷 267-272页

作者： MIAO YongWang1,2,WU GuiSheng3,WANG Lei2,LI DaLin4,TANG ShouKun5,LIANG JianPing2,MAO HuaMing2,LUO HuaiRong3 & ZHANG YaPing1,6 1 Laboratory for Conservation and Utilization of Bio-resource,Yunnan University,Kunming 650091,China 2 Faculty of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China 3 Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China 4 Yunnan Institute of Buffalo Science and Technology,Kunming 650021,China 5 Animal Husbandry and Veterinary Station of Luxi city,Luxi 678400,China 6 State Key Laboratory of Genetic Resources and Evolution,Kunming Institute of Zoology,Chinese Academy of Sciences,Kunming 650223,China Laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming China Faculty of Animal Science and Technology Yunnan Agricultural University Kunming China Kunming Institute of Botany Chinese Academy of Sciences Kunming China Yunnan Institute of Buffalo Science and Technology Kunming China Animal Husbandry and Veterinary Station of Luxi city Luxi China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming China

Melanocortin-1 receptor (MC1R) plays a major role in pigmentation in many *** investigate if the MC1R gene is associated with coat color in water buffalo,the coding region of MC1R gene of 216 buffalo samples was sequenced,which included 49 black river buffalo (Murrah and Nili-Ravi),136 swamp buffalo (Dehong,Diandongnan,Dechang,Guizhou,and Xilin) with white and gray body,and 31 hybrid offspring of river buffalo Nili-Ravi (or Murrah) and swamp *** the three variation sites found,SNP684 was synonymous,while SNP310 and SNP384 were nonsynonymous,leading to p.S104G and p.I128M changes,*** Individuals carrying homozygote EBR/EBR were *** genotype and phenotype analysis of the hybrid offspring of black river buffalo and gray swamp buffalo further revealed that the river buffalo type allele EBR or the allele carrying the amino acid p.104S was important for the full function of *** in silico functional analysis showed that the amino acid substitutions p.G104S and p.M128I had significant impact on the function of *** results indicate that the allele EBR or the allele carrying the amino acid p.104S was associated with the black coat color in buffalo.

关键词： Buffalo coat color MC1R

Decoding white coat hypertension

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世界临床病例杂志 2017年第3期5卷 82-92页

作者： Dennis A Bloomfield Alex Park Richmond University Medical Center

There is arguably no less understood or more intriguing problem in hypertension that the"white coat"condition,the standard concept of which is significantly blood pressure reading obtained by medical personnel of authoritative standing than that obtained by more junior and less authoritative personnel and by the patients *** hospital-initiated ambulatory blood pressure monitoring,the while effect manifests as initial and ending pressure elevations,and,in treated patients,a low daytime *** effect is essentially *** diastolic white coat hypertension appears to be exceedingly *** the basis of the studies,we believe that the white coat phenomenon is a common,periodic,neuro-endocrine reflex conditioned by anticipation of having the blood pressure taken and the fear of what this measurement may indicate concerning future *** does not change with time,or with prolonged association with the physician,particularly with advancing years,it may be superimposed upon essential hypertension,and in patients receiving hypertensive medication,blunting of the nighttime dip,which occurs in about half the patients,may be a compensatory mechanisms,rather than an indication of cardiovascular *** than the blunted dip,the morning surge or the widened pulse pressure,cardiovascular risk appears to be related to elevation of the average night time pressure.

关键词： White coat Ambulatory blood pressure Triggers Hypertension Neuro-endocrine reflex Nighttime dip Morning surge Conditioned reflex

Use of buffy coat thick films in detecting malaria parasites in patients with negative conventional thick films

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Asian Pacific Journal of Tropical Biomedicine 2012年第4期2卷 301-303页

作者： Chatnapa Duangdee Noppadon Tangpukdee Srivicha Krudsood Polrat Wilairatana Hospital for Tropical Diseases Bangkok Department of Clinical Tropical Medicine Faculty of Tropical Medicine Department of Tropical Hygiene Faculty of Tropical Medicine

Objective:To determine the frequency of malaria parasite detection from the buffy coal blood films by using capillary tube in falciparum malaria patients with negative conventional thick ***:Thirty six uncomplicated falciparum malaria patients confirmed by conventional thick and thin films were included in the *** patients were treated with artemisinin combination therapy at Hospital for Tropical Diseases,Bangkok,Thailand for 28 *** for conventional blood films were conducted every 6 hours until negative parasitemia,then daily fingerpricks for parasite checks were conducted until the patients were discharged from hospital. Blood samples were also concurrently collected in 3 heparinized capillary tubes at the same time of fingerpricks for conventional blood films when the prior parasitemia was negative on thin films and parasitemia was lower than 50 parasites/200 white blood cells by thick *** first negative conventional thick films were compared with buffy coat thick films for parasite identification. Results:Out of 36 patients with thick films showing negative for asexual forms of parasites, buffy coat films could detect remaining 10 patients(27.8%) with asexual forms of Plasmodium ***:The study shows that buffy coat thick films are useful and can detect malarial parasites in 27.8%of patients whose conventional thick films show negative parasitemia.

关键词： Malaria Detection Buffy coat Thick film Malaria parasite Falciparum malaria Plasmodium falciparum Parasitemia Microscopy

Tamarind seed coat extract restores reactive oxygen species through attenuation of glutathione level and antioxidant enzyme expression in human skin fibroblasts in response to oxidative stress

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Asian Pacific Journal of Tropical Biomedicine 2014年第5期4卷 379-385页

作者： Oranuch Nakchat Nonthaneth Nalinratana Duangdeun Meksuriyen Sunanta Pongsamart Department of Biochemistry and Microbiology Faculty of Pharmaceutical SciencesChulalongkorn University

Objective:To investigate the role and mechanism of tamarind seed coat extract(TSCE) on normal human skin fibroblast CCD-l064Sk cells under normal and oxidative stress conditions induced by hydrogen peroxide(H_2O_2).Methods:Tamarind seed coats were extracted with boiling water and then partitioned with ethyl acetate before the cell *** of TSCE on intracellular reactive oxygen species(ROS),glutathione(GSH) level,antioxidant enzymes such as superoxide dismulase(SOU),glutathione peroxidase(CPx) and catalase activity including antioxidant protein expression was ***:TSCE significantly attenuated intracellular ROS in the absence and presence of H_1O_2by increasing GSH level In the absence of H_2O_2,TSCE significantly enhanced SOD and catalase activity but did not affected on ***,TSCE significantly increased the protein expression of SOD and CPx in H_2O_2-treated ***:TSCE exhibited antioxidant activities by scavenging *** GSll level that could protect human skin fibroblast cells from oxidative *** results highlight the antioxidant mechanism of tamarind seed coat through an antioxidant enzyme system,the exlracl potentially benefits for health food and cosmeceutical application of tamarind seed coat.

关键词： Tamarindux indica Seed coat Antioxidant Reactive oxygen species Glutathione Antioxidant enzymes CCD-1064Sk cells

NUMERICAL SIMULATION OF TEMPERATURE FIELD FOR coat OF coat-CAST TECHNOLOGY

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Acta Metallurgica Sinica(English Letters) 2000年第2期13卷 669-673页

作者： Z. X. Lee, F. P. Huang and W. Y. Zhang Tianjin University, Tianjin 300072, China

Using Fourier's conduction equation, a mathematical model of the temperature field for the coat of coat-cast technology is established. The relationship between the thickness, quality of the coat and casting temperature of liquid iron is analyzed. The results show that the coat quality is reliable; the combination of the coat with the base is solid; because foe temperature gradient of the coat is not large, the surface, middle and bond area of the coat are melted completely and the thickness of the coat can reach 2.3mm by using self-melting spraying alloy powder when the casting temperature is higher than 1380℃. In addition, the agreement between the calculated and measured values is good. This paper is of significant meaning for coat-cast technology and the coat quality.

关键词： coat-cast coat temperature fieldn numerical simulation

Novel intron 2 polymorphism in the melanophilin gene is in Hardy-Weinberg equilibrium and is not associated with coat color in goats

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Open Journal of Genetics 2013年第3期3卷 195-200页

作者： Mufliat A. Adefenwa Brilliant O. Agaviezor Sunday O. Peters Matthew Wheto Oludotun J. Ekundayo Moses Okpeku Bola O. Oboh Khalid O. Adekoya Christian O. N. Ikeobi Marcos De Donato Bolaji N. Thomas Ikhide G. Imumorin Department of Animal Breeding and Genetics University of Agriculture Abeokuta Nigeria Department of Animal Science Cornell University Ithaca USA Department of Biomedical Sciences Rochester Institute of Technology Rochester USA Department of Cell Biology and Genetics University of Lagos Lagos Nigeria Department of Livestock Production Niger Delta University Amassomma Nigeria

Pigmentation plays important adaptation and physiological efficiency roles in animals. In the sequence of a 648 bp fragment representing intron 1, exon 2, and part of intron 2 of the MLPH mammalian pigmentation gene, we identified a novel g.469C> G mutation in intron 2, and genotyped it in 266 Nigerian goats using PCR-RFLP analysis. The C allele had frequencies of 0.9625, 0.9804 and 0.97405 in West African Dwarf (WAD),Sahel(SH) and Red Sokoto (RS) breeds, respectively. The G allele was the highest in WAD (0.0375), followed by RS (0.02595), and then SH (0.0196). Overall low FIS and FST and high Nm values demonstrate little differentiation within and among the goat breeds at this intronic locus. This g.469C> G polymorphism in MLPH gene is the first in any goat breed and also first in Nigerian goats. Our results suggest that this intronic SNP locus is maintained at Hardy-Weinberg equilibrium (P coat color may indicate its neutrality in goats.

关键词： coat Color Goats Melanophilin Nigeria Intron 2 SNP PCR-RFLP

cDNA CLONING OF GENE ENCODING coat PROTEIN OF CMV INFECTING TO BACCO PLANTS IN CHINA

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Chinese Science Bulletin 1990年第14期35卷 1209-1214页

作者：胡天华吴琳刘玮米景九潘乃穟陈章良 National Lab of Protein Engineering and Plant Genetic Engineering at Peking University Beijing 100871 PRC Molecular Genetics Lab at Beijing Agricultural University.

In recent years, the rapid development of techniques in plant genetic engineering has provided a new way for breeding virus-resistance plants. Powell et al. constructed the cDNA gene encoding the coat protein(CP)of tobacco mosaic virus(TMV)under the control of a plant constitutive promoter and transferred it into tobacco cells. Transgenic tobacco plants expressing TMV coat protein gene showed the resistance or delaying of disease symptom development. Subsequently, the same method was successfully used for some other viruses such as cucumber mosaic virus(CMV), alfalfa mosaic virus(ALMV), potato virus X(PVX)and potato virus Y(PVY).

关键词： cucumber mosaic virus virus coat protein gene plant engineering of virus-resistance