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Novel intron 2 polymorphism in the melanophilin gene is in Hardy-Weinberg equilibrium and is not associated with coat color in goats

Novel intron 2 polymorphism in the melanophilin gene is in Hardy-Weinberg equilibrium and is not associated with coat color in goats

作     者:Mufliat A. Adefenwa Brilliant O. Agaviezor Sunday O. Peters Matthew Wheto Oludotun J. Ekundayo Moses Okpeku Bola O. Oboh Khalid O. Adekoya Christian O. N. Ikeobi Marcos De Donato Bolaji N. Thomas Ikhide G. Imumorin 

作者机构:Department of Animal Breeding and Genetics University of Agriculture Abeokuta Nigeria Department of Animal Science Cornell University Ithaca USA Department of Biomedical Sciences Rochester Institute of Technology Rochester USA Department of Cell Biology and Genetics University of Lagos Lagos Nigeria Department of Livestock Production Niger Delta University Amassomma Nigeria 

出 版 物:《Open Journal of Genetics》 (遗传学期刊(英文))

年 卷 期:2013年第3卷第3期

页      面:195-200页

学科分类:1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主  题:Coat Color Goats Melanophilin Nigeria Intron 2 SNP PCR-RFLP 

摘      要:Pigmentation plays important adaptation and physiological efficiency roles in animals. In the sequence of a 648 bp fragment representing intron 1, exon 2, and part of intron 2 of the MLPH mammalian pigmentation gene, we identified a novel g.469C G mutation in intron 2, and genotyped it in 266 Nigerian goats using PCR-RFLP analysis. The C allele had frequencies of 0.9625, 0.9804 and 0.97405 in West African Dwarf (WAD),Sahel(SH) and Red Sokoto (RS) breeds, respectively. The G allele was the highest in WAD (0.0375), followed by RS (0.02595), and then SH (0.0196). Overall low FIS and FST and high Nm values demonstrate little differentiation within and among the goat breeds at this intronic locus. This g.469C G polymorphism in MLPH gene is the first in any goat breed and also first in Nigerian goats. Our results suggest that this intronic SNP locus is maintained at Hardy-Weinberg equilibrium (P 0.05) and the lack of association of this SNP with coat color may indicate its neutrality in goats.

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