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The miR319-Targeted GhTCP4 Promotes the Transition from cell elongation to Wall Thickening in Cotton Fiber

Molecular Plant 2020年第7期13卷 1063-1077页

作者： Jun-Feng Cao Bo Zhao Chao-Chen Huang Zhi-Wen Chen Ting Zhao Hong-Ru Liu Guan-Jing Hu Xiao-Xia Shangguan Chun-Min Shan- Ling-Jian Wang Tian-Zhen Zhang Jonathan F.Wendel Xue-Ying Guan Xiao-Ya Chen National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China Plant Stress Biology Center Institute of Plant Physiology and Ecology/CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Shanghai 200032China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Agronomy Department College of Agriculture and BiotechnologyZhejiang UniversityHangzhouZhejiang 310058China Department of Ecology Evolution and Organismal BiologyIowa State UniversityAmesIA 50011.USA Plant Science Research Center Shanghai Key Laboratory of Plant Functional Genomics and ResourcesShanghai Chenshan Botanical GardenShanghai 201602China

Plant cell growth involves a complex interplay among cell-wall expansion, biosynthesis, and, in specific tissues, secondary cell wall (SCW) deposition, yet the coordination of these processes remains elusive. Cotton fiber cells are developmentally synchronous, highly elongated, and contain nearly pure cellulose when mature. Here, we report that the transcription factor GhTCP4 plays an important role in balancing cotton fiber cell elongation and wall synthesis. During fiber development the expression of miR319 declines while GhTCP4 transcript levels increase, with high levels of the latter promoting SCW deposition. GhTCP4 interacts with a homeobox-containing factor, GhHOX3, and repressing its transcriptional activity. GhTCP4 and GhHOX3 function antagonistically to regulate cell elongation, thereby establishing temporal control of fiber cell transition to the SCW stage. We found that overexpression of GhTCP4A upregulated and accelerated activation of the SCW biosynthetic pathway in fiber cells, as revealed by transcriptome and promoter activity analyses, resulting in shorter fibers with varied lengths and thicker walls. In contrast, GhTCP4 downregulation led to slightly longer fibers and thinner cell walls. The GhHOX3–GhTCP4 complex may represent a general mechanism of cellular development in plants since both are conserved factors in many species, thus providing us a potential molecular tool for the design of fiber traits.

关键词： cell elongation secondary cell wall cotton fiber Gossypium hirsutum TCP microRNA

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Gene expression and metabolite profiles of cotton fiber during cell elongation and secondary cell wall synthesis

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cell Research 2007年第5期17卷 422-434页

作者： Jin-Ying Gou1,2,3 Ling-Jian Wang1,2 Shuang-Ping Chen4 Wen-Li Hu1,2 Xiao-Ya Chen1,2 1 National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China 2 CAS Center of Shanghai Chenshan Botanical Garden, 300 Fenglin Road, Shanghai 200032, China 3 Graduate School of Chinese Academy of Sciences, Shanghai 200032, China 4 Department of Computer Science and Technology, University of Science and Technology of China, Hefei 230026, China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China CAS Center of Shanghai Chenshan Botanical Garden Shanghai China Graduate School of Chinese Academy of Sciences Shanghai China Department of Computer Science and Technology University of Science and Technology of China Hefei China

Cotton fibers elongate rapidly after initiation of elongation, eventually leading to the deposit of a large amount ofcellulose. To reveal features of cotton fiber cells at the fast elongation and the secondary cell wall synthesis stages, wecompared the respective transcriptomes and metabolite profiles. Comparative analysis of transcriptomes by cDNA arrayidentified 633 genes that were differentially regulated during fiber development. Principal component analysis (PCA)using expressed genes as variables divided fiber samples into four groups, which are diagnostic of developmental *** grouping results are also found if we use non-polar or polar metabolites as variables for PCA of developingfibers. Auxin signaling, wall-loosening and lipid metabolism are highly active during fiber elongation, whereas cellulosebiosynthesis is predominant and many other metabolic pathways are downregulated at the secondary cell wall synthesisstage. Transcript and metabolite profiles and enzyme activities are consistent in demonstrating a specialization process ofcotton fiber development toward cellulose synthesis. These data demonstrate that cotton fiber cell at a certain stage has itsown unique feature, and developmental stages of cotton fiber cells can be distinguished by their transcript and metaboliteprofiles. During the secondary cell wall synthesis stage, metabolic pathways are streamed into cellulose synthesis.

关键词： cotton fiber transcriptome metabolite profile auxin cell elongation cellulose synthesis

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The unique cell elongation and division characteristics of Lysinibacillus guiyuensis *** electronic-waste contaminated river sediment

The unique cell elongation and division characteristics of L...

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第五届全国微生物资源学术暨国家微生物资源平台运行服务研讨会

作者： Chunjie Zhu Xingjuan Chen Guoping Sun Jun Guo Meiying Xu Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Institute of Microbiology School of bioscience and bioengineering South China University of Technology State Key Laboratory of Applied Microbiology(Ministry-Guangdong Province Jointly Breeding Base) South China

Lysiniabcillus ***32 was isolated from electronic-waste contaminated river sediment..It is Gram-positive,motile,and spherical *** peptidoglycan type of strain GY32 was A4a, which is based on *** G+C content was 43.2 mol%.Analysis of the 16S rRNA gene sequence indicated that GY32 was most closely related to Lysinibacillus sphaericus DSM28(with a

关键词： Lysinibacillus cell elongation cell division BDE209 biodegradation

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Light regulates cell elongation via chromatin remodeling mechanism

Light regulates cell elongation via chromatin remodeling mec...

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第十四届全国植物基因组学大会

作者： Yanjun Jing Dong Zhang Xin Wang Weijiang Tang Wanqing Wang Junling Huai Gang Xu Dongqin Chen Yunliang Li Rongcheng Lin Key Laboratory of Photobiology Institute of Botanythe Chinese Academy of Sciences

Photomorphogenesis is a critical plant developmental process that involves light-mediated transcriptome changes,histone modifications,and inhibition of hypocotyl ***,the chromatin-based regulatory mechanism underlying... 详细信息

关键词： light chromatin remodeling transcription factor cell elongation

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Nitrogen deficiency-induced decrease in cytokinins content promotes rice seminal root growth by promoting root meristem cell proliferation and cell elongation

Nitrogen deficiency-induced decrease in cytokinins content p...

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第十九届中国作物学会学术年会

作者： Yanchun Zhu Qi Wang Xiao Zou Yongjun Lin 尹昌喜 College of Plant Science and Technology Huazhong Agricultural University National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University

Rice (Oryza sativa L.) is one of the most important food crops in the *** the past 50 years,rice yield has steadily increased worldwide,partly owing to an increase in nitrogen (N) ***,at present,the average recovery efficiency of N fertilizer is only 33%at the field *** N input and low N use efficiency not only increase crop production costs but also cause severe environmental ***,decreasing N application is an important goal of sustainable ***,decreasing N application may lead to N deficiency and affect rice root growth,and the underlying mechanism by which N deficiency affects rice root growth is still poorly *** growth is influenced by several external and internal factors,including N availability and phytohormone *** evidences suggest that both N and cytokinin (CK) are involved in mediating root growth,however,the interaction mechanism between N and CK underlying this developmental plasticity is still poorly *** rice Nipponbare (Oryza sativa L.)was used in this *** seeds were sterilized,soaked and germinated,then the germinated rice seeds were grown in an artificial climate incubator with a 12-h light (29℃)/12-h dark (26℃) *** this study,the influence of N deficiency on CK metabolism in rice seminal roots was ***,the mechanism by which N and CK interact to regulate rice seminal root growth was *** results demonstrate that N deficiency reduces CK content by inhibiting CK biosynthesis and promoting CK degradation through down-regulation of CK biosynthesis genes and up-regulation of CK degradation genes,***,our results indicate that the N deficiency-induced decrease in CK content promotes rice seminal root growth not only by enhancing root meristem cell proliferation rate through increased root meristem cell number,but also by promoting root cell elongation through up-regulation of root-specific Os

关键词： cell elongation cell proliferation cytokinin nitrogen deficiency root meristem seminal root growth

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The Root Cap Determines Ethylene-Dependent Growth and Development in Maize Roots

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Molecular Plant 2008年第2期1卷 359-367页

作者： Achim Hahn Roman Zimmermann Dierk Wanke Klaus Harter Hans G. Edelmann Botanisches Institut Universitfit zu Koin Gyrhofstr. 15 D-50931 Koin Germany Present address: ZMBP Pflanzenphysiologie University TQbingen Auf der Morgenstelle 1 D-72076 TQbingen Germany Institut fur Entwicklungsbiologie Universitat zu Koin Gyrhofstr. 17 D-50931 Koin Germany Institut fur Entwicklungsbiologie Universitat zu Koin Gyrhofstr. 17 D-50931 Koln Germany ZMBP Pflanzenphysiologie Universitat Tubingen Auf der Morgenstelle 1 D-72076 Tubingen Germany Present address: Biologie und ihre Didaktik Universitat Siegen D-57068 Siegen Germany

Besides providing protection against mechanical damage to the root tip, the root cap is involved in the perception and processing of diverse external and internal stimuli resulting in altered growth and development, The transduction of these stimuli includes hormonal signaling pathways such as those of auxin, ethylene and cytokinin. Here, we show that the root cap is essential for the ethylene-induced regulation of elongation growth and root hair formation in maize. Exogenously applied ethylene is no longer able to inhibit elongation growth when the root cap has been surgically removed prior to hormone treatment. Reconstitution of the cap positively correlates with the developing capacity of the roots to respond to ethylene again. In contrast, the removal of the root cap does not per se affect growth inhibition controlled by auxin and cytokinin. Furthermore, our semi-quantitative RT-PCR results support earlier findings that the maize root cap is a site of high gene expression activity with respect to sensing and responding to hormones such as ethylene, From these data, we propose a novel function of the root cap which is the establishment of competence to respond to ethylene in the distal zones of the root.

关键词： AUXIN BIOSYNTHESIS INSENSITIVE MUTANTS cell elongation

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Analysis of Five Differentially Expressed Gene Families in Fast Elongating Cotton Fiber

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 51-56页

作者： Jian-Xun FENG, Sheng-Jian JI, Yong-Hui SHI, Yu XU, Gang WEI, and Yu-Xian ZHU( National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China ) National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0-20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family -AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer proteins may contribute directly to the elongation and development of fiber cells.

关键词： cotton fiber cDNA array cell elongation arabinogalactan protein lipid transfer protein

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Identification and characterization of plasma membrane aquaporins isolated from fiber cells of Calotropis procera

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第7期14卷 586-595页

作者： Usman ASLAM Asia KHATOON Hafiza Masooma Naseer CHEEMA Aftab BASHIR National Institute for Biotechnology and Genetic Engineering (NIBGE) P.O. Box 577 Jhang Road Faisalabad 38000 Pakistan Department of Plant Breeding and Genetics University of Agriculture Faisalabad 38040 Pakistan)

Calotropis procera, commonly known as "milkweed", possesses long seed trichomes for seed dispersal and has the ability to survive under harsh conditions such as drought and salinity. Aquaporins are water channel proteins expressed in all land plants, divided into five subfamilies plasma membrane intrinsic proteins (PIPs), tonoplast intrinsic proteins (TIPs), NOD26-like proteins (NIPs), small basic intrinsic proteins (SIPs), and the unfamiliar X intrinsic proteins (XIPs). PIPs constitute the largest group of water channel proteins that are involved in different developmental and regulatory mechanisms including water permeability, cell elongation, and stomata opening. Aquaporins are also involved in abiotic stress tolerance and cell expansion mechanisms, but their role in seed trichomes (fiber cells) has never been investigated. A large number of clones isolated from C. procera fiber cDNA library showed sequence homology to PIPs. Both expressed sequence tags (ESTs) and real-time polymerase chain reaction (PCR) studies revealed that the transcript abundance of this gene family in fiber cells of C. procera is greater than that of cotton. Full-length cDNAs of CpPIP1 and CpPIP2 were isolated from C. procera fiber cDNA library and used for constructing plant expression vectors under constitutive (2×35S) and trichome-specific (GhLTP3) promoters. Transgenic tobacco plants were developed via Agrobacterium-mediated transformation. The phenotypic characteristics of the plants were observed after confirming the integration of transgene in plants. It was observed that CpPIP2 expression cassette under 2×35S and GhLTP3 promoter enhanced the numbers of stem and leave trichomes. However, 2×35S::CpPIP2 has a more amplified effect on trichome density and length than GhLTP3::CpPIP2 and other PIP constructs. These findings imply the role of C. procera PIP aquaporins in fiber cell elongation. The PIPs-derived cell expansion mechanism may be exploited through transgenic approaches for impr

关键词： Seed trichome Plasma membrane intrinsic protein (PIP) Fiber quality cell elongation Tobacco Agrobacterium

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Disruption of Cortical Microtubules by Overexpression of Green Fluorescent Protein-Tagged a-Tubulin 6 Causes a Marked Reduction in cell Wall Synthesis

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Journal of Integrative Plant Biology 2006年第1期48卷 85-98页

作者： David H. Burk Ruiqin Zhong W. Herbert Morrison III Zheng-Hua Ye Department of Plant Biology University of Georgia Athens Georgia 30602 USA Richard B. Russell Agriculture Research Center U.S. Department of Agriculture Agricultural Research Service Athens Georgia 30604 USA

It has been known that the transverse orientation of cortical microtubules （MTs） along the elongation axis is essential for normal cell morphogenesis, but whether cortical MTs are essential for normal cell wall synthesis is still not clear. In the present study, we have investigated whether cortical MTs affect cell wall synthesis by direct alteration of the cortical MT organization in Arabidopsis thaliana. Disruption of the cortical MT organization by expression of an excess amount of green fluorescent protein-tagged a-tubulin 6 （GFP-TUA6） in transgenic Arabidopsis plants was found to cause a marked reduction in cell wall thickness and a de- crease in the cell wall sugars glucose and xylose. Concomitantly, the stem strength of the GFP-TUA6 overexpressors was markedly reduced compared with the wild type. In addition, expression of excess GFP- TUA6 results in an alteration in cell morphogenesis and a severe effect on plant growth and development. Together, these results suggest that the proper organization of cortical MTs is essential for the normal synthesis of plant cell walls.

关键词： Arabidopsis thaliana cell elongation cell wall synthesis cortical microtubules microtubule organization.

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Brassinosteroid Biosynthetic Gene CmDWF4 Regulates Bud Outgrowth in Chrysanthemum morifolium

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Phyton-International Journal of Experimental Botany 2023年第6期92卷 1681-1694页

作者： Xianrong Fu Aiping Song Bo Peng Song Li Weixin Liu Lingling Zhang Jiafu Jiang Sumei Chen Fadi Chen State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization Key Laboratory of LandscapingKey Laboratory of Flower Biology and Germplasm Innovation(South)Ministry of Agriculture and Rural AffairsKey Laboratory of Biology of Ornamental Plants in East ChinaNational Forestry and Grassland AdministrationCollege of HorticultureNanjing Agricultural UniversityNanjing210095China

Brassinosteroids(BRs),a class of steroid phytohormones,play a critical role in plant growth and *** DWF4 gene encodes a cytochrome P450 enzyme(CYP90B1),which is considered a rate-limiting enzyme in BR ***,we identified a homologous gene of DWF4 in chrysanthemum,*** gene was predicted to encode 491 amino acid residues with a molecular weight of 56.2 kDa and an isoelectric point(pI)of *** of CmDWF4 in chrysanthemum was found to significantly increase growth rate,number,and length of lateral *** analysis showed that multiple xyloglucan endotransglycosylase/hydrolase(XTH)family encoding genes associated with cell wall modification were up-regulated in CmDWF4-overexpressing ***-PCR assay confirmed the up-regulation of CmXTH6,CmXTH23,and CmXTH28 in CmDWF4-overexpression ***,this work establishes a mechanism by which BR biosynthetic gene CmDWF4 promotes lateral bud outgrowth in chrysanthemum,possibly through regulating cell elongation and expansion.

关键词： BR axillary bud shoot branching XTHs cell elongation

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