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An atlas of immune cell transcriptomes in human immunodeficiency virus-infected immunological non-responders identified marker genes that control viral replication

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Chinese Medical Journal 2023年第22期136卷 2694-2705页

作者： Yahong Chen Xin Li Shuran Liu Wen Ao Jing Lin Zhenting Li Shouli Wu Hanhui Ye Xiao Han Dongliang Li Department of infection Mengchao Hepatobiliary Hospital of Fujian Medical UniversityFuzhouFujian 350025China Department of infection Fuzong Clinical Medical College of Fujian Medical UniversityFuzhouFujian 362002China College of Biological Science and Engineering Fuzhou UniversityFuzhouFujian 350025China Fujian Provincial Center for Disease Control and Prevention No.76 Jintai RoadFuzhouFujian 350001China Department of Hepatobiliary Medicine 900th Hospital of Joint Logistics Support Forces of the Chinese PLAFuzhouFujian 350000China

Background:Previous studies have examined the bulk transcriptome of peripheral blood immune cells in acquired immunodeficiency syndrome patients experiencing immunological non-responsiveness.This study aimed to investigate the characteristics of specific immune cell subtypes in acquired immunodeficiency syndrome patients who exhibit immunological non-responsiveness.Methods:A single-cell transcriptome sequencing of peripheral blood mononuclear cells obtained from both immunological responders(IRs)(cd4^(+)T-cell count>500)and immunological non-responders(INRs)(cd4^(+)T-cell countcd16^(+)monocytes,and exhausted B cells demonstrated the most substantial differences between INRs and IRs,with fold changes of 39.79,11.08,and 2.71,respectively.In contrast,the cd4^(+)T cell ratio was significantly decreased(0.39-fold change)in INRs compared with that in IRs.Similarly,the ratios of natural killer cells and terminal effector CD8^(+)T cells were also lower(0.37-fold and 0.27-fold,respectively)in the INRs group.In addition to several well-characterized immune cell-specific markers,we identified a set of 181 marker genes that were enriched in biological pathways associated with human immunodeficiency virus(HIV)replication.Notably,ISG15,IFITM3,PLSCR1,HLA-DQB1,CCL3L1,and DDX5,which have been demonstrated to influence HIV replication through their interaction with viral proteins,emerged as significant monocyte marker genes.Furthermore,the differentially expressed genes in natural killer cells were also enriched in biological pathways associated with HIV replication.Conclusions:We generated an atlas of immune cell transcriptomes in HIV-infected IRs and INRs.Host genes associated with HIV replication were identified as mark

关键词： Single-cell transcriptome sequencing Human immunodeficiency virus Immunological non-responsiveness cd4 T cell CD8 T cell Natural killer cells HIV replication

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肿瘤引流淋巴结抗原特异性记忆CD8^(+)T细胞是PD-1/PD-L1免疫检查点阻断治疗的关键响应者

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科学通报 2022年第35期67卷 4152-4154页

作者：黄启钊吴霞王志明陈香宇孙倍成许力凡唐忠辉叶丽林南方医科大学广东省免疫治疗与免疫调节重点实验室广州510515 中山大学中山医学院广州510080 陆军军医大学全军免疫学研究所重庆400038 南京大学医学院附属鼓楼医院南京210008 昌平国家实验室北京102206

CD8^(+)T细胞耗竭是机体免疫系统不能有效清除慢性病毒感染及恶性肿瘤的关键原因.相较于急性感染或者疫苗免疫情形下诱导形成的记忆性CD8^(+)T细胞,耗竭的CD8^(+)T细胞表面持续表达包括PD-1在内的免疫检查点分子,其增殖潜力、效应及杀... 详细信息

CD8^(+)T细胞耗竭是机体免疫系统不能有效清除慢性病毒感染及恶性肿瘤的关键原因.相较于急性感染或者疫苗免疫情形下诱导形成的记忆性CD8^(+)T细胞,耗竭的CD8^(+)T细胞表面持续表达包括PD-1在内的免疫检查点分子,其增殖潜力、效应及杀伤功能等都较为低下,且易于凋亡.

关键词：慢性病毒感染杀伤功能抗原特异性机体免疫系统免疫检查点 T细胞 CD8 记忆性

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Aging induces severe SIV infection accompanied by an increase in follicular CD8+T cells with overactive STAT3 signaling

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Cellular & Molecular Immunology 2022年第9期19卷 1042-1053页

作者： Hong-Yi Zheng Xue-Hui Wang Xiao-Yan He Min Chen Ming-Xu Zhang Xiao-Dong Lian Jia-Hao Song Yan Hu Wei Pang Yun Wang Zheng-Fei Hu Long-Bao Lv Yong-Tang Zheng Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common DiseasesCenter for Biosafety Mega-ScienceKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan650223China School of Life Sciences University of Science and Technology of ChinaHefeiAnhui230026China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan650204China National Resource Center for Non-Human Primates National Research Facility for Phenotypic&Genetic Analysis of Model Animals(Primate Facility)Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan650107China

The number of elderly people living with HIV is increasing globally,and the condition of this population is relatively complicated due to the dual effects of aging and HIV infection.However,the impact of HIV infection combined with aging on the immune homeostasis of secondary lymphoid organs remains unclear.Here,we used the simian immunodeficiency virus mac239(SIVmac239)strain to infect six young and six old Chinese rhesus macaques(ChRMs)and compared the infection characteristics of the two groups in the chronic stage through multiplex immunofluorescence staining of lymph nodes.The results showed that the SIV production and cd4/CD8 ratio inversion in old ChRMs were more severe than those in young ChRMs in both the peripheral blood and the lymph nodes,especially when a large number of CD8+T cells infiltrated the follicles and germinal centers.STAT3 in these follicular CXCR5+CD8+T cells was highly activated,with high expression of granzyme B,which might be caused by the severe inflammatory milieu in the follicles of old ChRMs.This study indicates that aging may be a cofactor involved in SIV-induced immune disorders in secondary lymphoid tissues,affecting the effective antiviral activity of highly enriched follicular CXCR5+CD8+cells.

关键词： SIV Aging Follicle CD8 T cells

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Artificial intelligence-based analysis of tumor-infiltrating lymphocyte spatial distribution for colorectal cancer prognosis

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Chinese Medical Journal 2024年第4期137卷 421-430页

作者： Ming Cai Ke Zhao Lin Wu Yanqi Huang Minning Zhao Qingru Hu Qicong Chen Su Yao Zhenhui Li Xinjuan Fan Zaiyi Liu Department of Radiology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Guangdong Provincial Key Laboratory of Artificial Intelligence in Medical Image Analysis and Application GuangzhouGuangdong 510080China Guangdong Cardiovascular Institute Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Medical Research Institute Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Department of Pathology The Third Affiliated Hospital of Kunming Medical UniversityYunnan Cancer HospitalYunnan Cancer CenterKunmingYunnan 650118China The Second School of Clinical Medicine Southern Medical UniversityGuangzhouGuangdong 510515China Institute of Computing Science and Technology Guangzhou UniversityGuangzhouGuangdong 510006China Department of Pathology Guangdong Provincial People’s Hospital(Guangdong Academy of Medical Sciences)Southern Medical UniversityGuangzhouGuangdong 510080China Department of Pathology The Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhouGuangdong 510655China

Background:Artificial intelligence(AI)technology represented by deep learning has made remarkable achievements in digital pathology,enhancing the accuracy and reliability of diagnosis and prognosis evaluation.The spatial distribution of cd3^(+)and CD8^(+)T cells within the tumor microenvironment has been demonstrated to have a significant impact on the prognosis of colorectal cancer(CRC).This study aimed to investigate cd3_(CT)(cd3^(+)T cells density in the core of the tumor[CT])prognostic ability in patients with CRC by using AI technology.Methods:The study involved the enrollment of 492 patients from two distinct medical centers,with 358 patients assigned to the training cohort and an additional 134 patients allocated to the validation cohort.To facilitate tissue segmentation and T-cells quantification in whole-slide images(WSIs),a fully automated workflow based on deep learning was devised.Upon the completion of tissue segmentation and subsequent cell segmentation,a comprehensive analysis was conducted.Results:The evaluation of various positive T cell densities revealed comparable discriminatory ability between cd3_(CT) and cd3-CD8(the combination of cd3^(+)and CD8^(+)T cells density within the CT and invasive margin)in predicting mortality(C-index in training cohort:0.65 vs.0.64;validation cohort:0.69 vs.0.69).The cd3_(CT) was confirmed as an independent prognostic factor,with high cd3_(CT) density associated with increased overall survival(OS)in the training cohort(hazard ratio[HR]=0.22,95%confidence interval[CI]:0.12–0.38,Pcd3^(+)and CD8^(+)T cells within tissue regions in WSIs using AI technology.The cd3_(CT) confirmed as a stage-independent predictor for OS in CRC patients.Moreover,cd3_(CT) shows promise in simplifying the cd3-CD8 system and facilitating its practical application in clinical settings.

关键词： Colorectal cancer Artificial intelligence Deep learning Digital pathology Prognosis Immune cells cd3 CD8 TME

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Artificial intelligence-based comprehensive analysis of immune-stemness-tumor budding profile to predict survival of patients with pancreatic adenocarcinoma

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Cancer Biology & Medicine 2023年第3期20卷 196-217页

作者： Tianxing Zhou Quan Man Xueyang Li Yongjie Xie Xupeng Hou Hailong Wang Jingrui Yan Xueqing Wei Weiwei Bai Ziyun Liu Jing Liu Jihui Hao Department of Pancreatic Cancer Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Department of Hepatopancreatobiliary Surgery Tongliao City HospitalTongliao 028000China Department of Breast Oncoplastic Surgery Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerKey Laboratory of Breast Cancer Prevention and TherapyTianjin Medical UniversityMinistry of EducationTianjin 300060China Department of Cancer Cell Biology Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerKey Laboratory of Breast Cancer Prevention and TherapyTianjin Medical UniversityMinistry of EducationTianjin 300060China Department of Diagnostic and Therapeutic Ultrasonography Tianjin Medical University Cancer Institute&HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerKey Laboratory of Breast Cancer Prevention and TherapyTianjin Medical UniversityMinistry of EducationTianjin 300060China

Objective:Pancreatic ductal adenocarcinoma(PDAC)is an aggressive malignancy.CD8^(+)T cells,cancer stem cells(CSCs),and tumor budding(TB)have been significantly correlated with the outcome of patients with PDAC,but the correlations have been independently reported.In addition,no integrated immune-CSC-TB profile for predicting survival in patients with PDAC has been established.Methods:Multiplexed immunofluorescence and artificial intelligence(AI)-based comprehensive analyses were used for quantification and spatial distribution analysis of CD8^(+)T cells,cd133^(+)CSCs,and TB.In vivo humanized patient-derived xenograft(PDX)models were established.Nomogram analysis,calibration curve,time-dependent receiver operating characteristic curve,and decision curve analyses were performed using R software.Results:The established‘anti-/pro-tumor’models showed that the CD8^(+)T cell/TB,CD8^(+)T cell/cd133^(+)CSC,TB-adjacent CD8^(+)T cell,and cd133^(+)CSC-adjacent CD8^(+)T cell indices were positively associated with survival of patients with PDAC.These findings were validated using PDX-transplanted humanized mouse models.An integrated nomogram-based immune-CSC-TB profile that included the CD8^(+)T cell/TB and CD8^(+)T cell/cd133^(+)CSC indices was established and shown to be superior to the tumor-nodemetastasis stage model in predicting survival of patients with PDAC.Conclusions:‘Anti-/pro-tumor’models and the spatial relationship among CD8^(+)T cells,CSCs,and TB within the tumor microenvironment were investigated.Novel strategies to predict the prognosis of patients with PDAC were established using AI-based comprehensive analysis and machine learning workflow.The nomogram-based immune-CSC-TB profile can provide accurate prognosis prediction for patients with PDAC.

关键词： Artificial intelligence CD8 CSCs tumor budding PDAC nomogram

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肝移植术后发生急性排斥反应受者淋巴细胞亚群的变化及意义

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器官移植 2022年第4期13卷 509-515页

作者：王若麟李瀚贾亚男许文犁李先亮贺强朱继巧首都医科大学附属北京朝阳医院肝胆胰脾外科北京100020 中国医学科学院肿瘤医院

目的探讨肝移植受者术后发生急性排斥反应时淋巴细胞亚群的变化及意义。方法选取接受肝移植且发生急性排斥反应的受者作为排斥组(17例),利用倾向评分匹配方法按1∶1比例选取肝功能稳定的肝移植受者作为对照组(17例)。分析肝移植术后急... 详细信息

目的探讨肝移植受者术后发生急性排斥反应时淋巴细胞亚群的变化及意义。方法选取接受肝移植且发生急性排斥反应的受者作为排斥组(17例),利用倾向评分匹配方法按1∶1比例选取肝功能稳定的肝移植受者作为对照组(17例)。分析肝移植术后急性排斥反应的发生情况,对比两组受者他克莫司浓度。比较两组受者外周血淋巴细胞亚群的绝对值和比例,采用受试者工作特征(ROC)曲线分析淋巴细胞亚群对肝移植术后急性排斥反应发生的诊断价值。比较排斥组治疗前后淋巴细胞亚群绝对值和比例的变化。结果排斥组17例受者中,4例在术后28 d内发生急性排斥反应,13例在术后29~180 d发生急性排斥反应。两组他克莫司谷浓度差异无统计学意义(P=0.295)。与对照组比较,排斥组受者外周血T细胞、cd4+T细胞、B细胞和自然杀伤(NK)T细胞的比例均上升(均为Pcd4+T细胞、B细胞和NKT细胞比例的曲线下面积(AUC)分别为0.76、0.73和0.77。联用cd4+T细胞、B细胞和NKT细胞比例的AUC为0.89,当临界值为0.69时,灵敏度为0.706,特异度为0.941。排斥组所有受者治疗后均逐渐恢复,最终肝功能正常,治疗后T细胞、cd4+T细胞、CD8+T细胞和NK细胞比例均降低(均为Pcd4+T细胞、B细胞和NKT细胞比例可早期发现和诊断肝移植术后急性排斥反应。

关键词：淋巴细胞亚群急性排斥反应肝移植 T细胞 B细胞自然杀伤细胞 cd4 CD8 树突状细胞自然杀伤T细胞

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结直肠癌中IL-38与cd4、CD8的相关性及临床意义

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临床与实验病理学杂志 2023年第5期39卷 531-536页

作者：袁柳红陈飞儿谈振宇张凤赵一鸣包士三陶琨上海交通大学医学院附属同仁医院病理科上海200050

目的探讨IL-38在结直肠癌(colorectal cancer,CRC)组织中的表达与cd4、CD8表达的相关性及与预后的关系。方法收集314例CRC临床资料,采用免疫组化SP法检测IL-38、cd4、CD8、IL-6、IL-10、IL-23和TGF-β的表达,分析其临床病理特征并复习... 详细信息

目的探讨IL-38在结直肠癌(colorectal cancer,CRC)组织中的表达与cd4、CD8表达的相关性及与预后的关系。方法收集314例CRC临床资料,采用免疫组化SP法检测IL-38、cd4、CD8、IL-6、IL-10、IL-23和TGF-β的表达,分析其临床病理特征并复习相关文献。结果IL-38在癌组织(H score:166.7)中的表达水平明显低于癌旁组织(H score:194.4)(P8与CD8表达呈正相关(r=0.1154,P=0.0392),IL-38与cd4表达无明显相关性(r=0.0249,P=0.6499)。IL-38与IL-10(r=-0.1129,P=0.0394)、IL-23(r=-0.1126,P=0.0362)、TGF-β(r=-0.1618,P=0.003)表达呈负相关。IL-38和CD8双高表达的患者3年生存率,明显高于IL-38和CD8单高表达或双低表达的患者(P=0.0363)。Cox多因素生存分析:IL-38与CD8双高表达是CRC患者预后良好的独立影响因素(HR=0.175,95%CI:0.037~0.826,P=0.028)。结论IL-38和CD8+T细胞可能通过调节肿瘤微环境影响CRC的进展,联合评估IL-38与CD8的表达是CRC患者术后的独立预测因素。

关键词：结直肠肿瘤 IL-38 cd4 CD8 预后生存分析

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脑型疟炎症诱导CXCL10神经毒性星形胶质细胞产生及其机制研究

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中国寄生虫学与寄生虫病杂志 2023年

作者：仝国栋朱卿昊王军刘晓冉沈燕梁姣李英辉黄豫晓王一赵亚空军军医大学基础医学院微生物与病原生物学教研室西北大学生命科学学院空军军医大学基础医学院

目的探索在脑型疟(cerebral malaria,CM)发病过程中，星形胶质细胞(astrocyte,AS)被脑血管周炎性微环境诱导活化及其机制，及其对神经元损伤的影响。方法分离原代星形胶质细胞，模拟脑型疟发病过程中脑血管周炎性微环境，以γ干扰素(I... 详细信息

目的探索在脑型疟(cerebral malaria,CM)发病过程中，星形胶质细胞(astrocyte,AS)被脑血管周炎性微环境诱导活化及其机制，及其对神经元损伤的影响。方法分离原代星形胶质细胞，模拟脑型疟发病过程中脑血管周炎性微环境，以γ干扰素(IFN-γ)、肿瘤坏死因子α (TNF-α)和感染疟原虫的红细胞(parasitized-RBCs,pRBCs)诱导星形胶质细胞活化；对活化星形胶质细胞进行转录组测序分析及评价；分离脑型疟小鼠脾脏CD8+T细胞，与活化的星形胶质细胞共孵育，评价其维持CD8+T细胞活化的能力；分离原代神经元，检测星形胶质细胞培养上清对神经元损伤的影响；初步探索脑血管周炎性微环境诱导星形胶质细胞活化的分子机制。结果体外模拟血管周炎性微环境可诱导星形胶质细胞活化，并伴随胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)的上调(P CD80、CD86、MHCⅠ表达水平显著提高，MHCⅡ表达水平无明显变化；CXCL10highAS与CD8+T细胞形成“免疫突触”样结构，可维持CD8+T细胞持续活化。CCK-8细胞活力和LDH释放检测显示，CXCL10highAS上清可降低神经元活力(P 0.05)；免疫荧光结果显示，CXCL10highAS上清可诱导神经元胞体收缩、轴突断裂。Western blotting结果显示，CXCL10highAS中STAT1及其磷酸化水平显著升高(P 0.05);STAT1抑制剂可显著缓解CXCL10highAS介导的神经元损伤。结论体外模拟实验型脑型疟发病过程中血管周炎性微环境可通过STAT1分子诱导神经毒性星形胶质细胞产生，可导致神经元死亡；该神经毒性星形胶质细胞与活化的CD8+T细胞相互作用，进一步加重中枢神经系统(central nervous system,CNS)损伤；该神经毒性星形胶质细胞有望作为疾病治疗的靶点。

关键词：脑型疟星形胶质细胞 CD8 T细胞神经元炎性微环境

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Proteomic analysis reveals distinctive protein profiles involved in CD8^(+) T cell-mediated murine autoimmune cholangitis

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Cellular & Molecular Immunology 2018年第8期15卷 756-767页

作者： Weici Zhang Ren Zhang Jun Zhang Ying Sun Patrick SC Leung Guo-Xiang Yang Zongwen Shuai William M Ridgway M Eric Gershwin Division of Rheumatology Allergy and Clinical ImmunologyUniversity of CaliforniaDavisCaliforniaUSA Department of Pathogenic Biology and Immunology Guangzhou University of Chinese MedicineGuangzhou 510006China Center for non-infectious liver diseases Beijing 302 HospitalBeijing 100039China Department of Rheumatology and Immunology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022China Division of Immunology Allergy and RheumatologyUniversity of CincinnatiCincinnatiOH 45267

Autoimmune cholangitis arises from abnormal innate and adaptive immune responses in the liver,and T cells are critical drivers in this process.However,little is known about the regulation of their functional behavior during disease development.We previously reported that mice with T cell-restricted expression of a dominant negative form of transforming growth factor beta receptor type Ⅱ(dnTGFβ RII)spontaneously develop an autoimmune cholangitis that resembles human primary biliary cholangitis(PBC).Adoptive transfer of CD8^(+) but not cd4^(+) T cells into Rag1^(−/−)mice reproduced the disease,demonstrating a critical role for CD8^(+) T cells in PBC pathogenesis.Herein,we used SOMAscan technology to perform proteomic analysis of serum samples from dnTGFβRII and B6 control mice at different ages.In addition,we analyzed CD8 protein profiles after adoptive transfer of splenic CD8^(+) cells into Rag1^(−/−)recipients.The use of the unique SOMAscan aptamer technology revealed critical and distinct profiles of CD8 cells,which are key to biliary mediation.In total,254 proteins were significantly increased while 216 proteins were significantly decreased in recipient hepatic CD8^(+) cells compared to donor splenic CD8^(+) cells.In contrast to donor splenic CD8^(+) cells,recipient hepatic CD8^(+) cells expressed distinct profiles for proteins involved in chemokine signaling,focal adhesion,T cell receptor and natural killer cell-mediated cytotoxicity pathways.

关键词： autoimmune cholangitis CD8 dnTGFβRII mice proteomic analysis

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癸酸通过活化CD8T细胞促进小鼠黑色素瘤B16F10细胞皮下荷瘤模型的抗肿瘤免疫反应

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中国肿瘤生物治疗杂志 2024年

作者：张翀金海振周纯胡惠惠王娟王秦兰上海市胸科医院/上海交通大学医学院附属胸科医院科教科上海市胸科医院/上海交通大学医学院附属胸科医院放疗科上海市胸科医院/上海交通大学医学院附属胸科医院重症医学科上海市胸科医院/上海交通大学医学院附属胸科医院呼吸与危重症医学科上海市胸科医院/上海交通大学医学院附属胸科医院中心实验室

目的：研究中链脂肪酸癸酸(Decanoic acid)对CD8+T细胞活化的影响，及其对CD8+T细胞介导的抗肿瘤免疫反应的作用和机制。方法：在小鼠黑色素瘤B16F10皮下荷瘤模型中，癸酸灌胃后检测小鼠肿瘤生长以及生存率；并用流式细胞术检测肿瘤微... 详细信息

目的：研究中链脂肪酸癸酸(Decanoic acid)对CD8+T细胞活化的影响，及其对CD8+T细胞介导的抗肿瘤免疫反应的作用和机制。方法：在小鼠黑色素瘤B16F10皮下荷瘤模型中，癸酸灌胃后检测小鼠肿瘤生长以及生存率；并用流式细胞术检测肿瘤微环境中浸润CD8+T细胞的活化水平。在B16F10-OVA和OT-I T 细胞共培养体系，采用流式细胞术检测癸酸对CD8+T细胞肿瘤杀伤水平的影响。采用α-CD8清除B16F10荷瘤小鼠体内CD8+T细胞，检测小鼠肿瘤体积大小。在癸酸处理的小鼠原代CD8+T细胞中，采用WB、ELISA及q-PCR、以及流式细胞术检测T细胞受体(TCR)活化、效应细胞因子产生以及增殖和代谢水平。在B16F10荷瘤小鼠模型中，采用α-PD-1联合癸酸给药检测小鼠肿瘤生长以及生存率。结果：在小鼠黑色素瘤荷瘤模型中，与对照组相比，癸酸显著抑制小鼠肿瘤生长并提高其生存率(均PCD8+T细胞IFN-γ和TNF-α的产生显著高于对照组(PCD8清除CD8+T细胞后，癸酸对肿瘤的抑制作用显著降低(PCD8+T细胞后，TCR活化水平显著升高、细胞因子IL-2和IFN-γ的产生增多、线粒体代谢Mitotracker的表达显著上调(均PCD8+T细胞活化，增强其抗肿瘤免疫反应。

关键词：抗肿瘤免疫癸酸 CD8 T细胞 PD-1

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