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Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry traces the geographical source of Biomphalaria pfeifferi and bulinus forskali,involved in schistosomiasis transmission

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Infectious Diseases of Poverty 2024年第1期13卷 98-99页

作者： Papa Mouhamadou Gaye EI Hadj Ibrahima Ndiaye Souleymane Doucoure Doudou Sow Mapenda Gaye Ndiaw Goumballa Carole Cassagne Coralie L'Ollivier Oleg Medianikov Cheikh Sokhna Stephane Ranque Aix-Marseille University IRD AP-HM SSA VITROME Marseille France VITROME International IRD-UCAD Campus Dakar Senegal Hospital-University Institut (IHU) Mediterranée Infection Marseille France Departement of Animal Biology Faculty of Sciences and Techniques UCAD Dakar Senegal Department of Parasitology-Mycology UFR Sciences de la Santé Université Gaston Berger Saint-Louis Senegal

Background Freshwater snails of the genera bulinus spp.,Biomphalaria spp.,and Oncomelania *** the main intermediate hosts of human and animal *** of these snails has long been based on mor-phological and/or genomic criteria,which have their *** limitations include a lack of precision for the morphological tool and cost and time for the DNA-based ***,Matrix-Assisted Laser Desorp-tion/lonization Time-Of-Flight(MALDI-TOF)mass spectrometry,a new tool used which is routinely in clinical microbi-ology,has emerged in the field of malacology for the identification of freshwater *** study aimed to evaluate the ability of MALDI-TOF MS to identify Biomphalaria pfeifferi and bulinus forskali snail populations according to their *** This study was conducted on 101 *** and 81 *** snails collected in three distinct geo-graphical areas of Senegal(the North-East,South-East and central part of the country),and supplemented with wild and laboratory *** which had previously been morphologically described were identified by MALDl-TOF MS[identification log score values(LSV)≥1.7],after an initial blind test using the pre-existing *** DNA-based identification,new reference spectra of ***(n=10)and ***(n=5)from the geographical areas were added to the MALDI-TOF spectral *** final blind test against this updated database was per-formed to assess identification at the geographic source *** MALDI-TOF MS correctly identified 92.1%of 101 *** snails and 98.8%of 81 *** *** the final blind test,88%of 166 specimens were correctly identified according to both their species and sampling site,with LSVs ranging from 1.74 to *** geographical source was adequately identified in 90.1%of 91 *** and 85.3%of 75 *** *** Our findings demonstrate that MALDI-TOF MS can identify and differe

关键词： Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Snail Intermediate host Biomphalaria pfeifferi bulinus forskali Schistosomiasis Senegal

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