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Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and buffy Coat <i>in Vitro</i>

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Journal of Biosciences and Medicines 2018年第3期6卷 86-94页

作者： Longhao Jin Zhongye Zhang Yanqun Liu Kumar Gurung Yongjun Jin Yuanming He Yu Jin Zhehu Jin Department of Orthopedic Surgery Yanbian University Hospital Yanji City China Department of Orthopedic Surgery Songyuan Central Hospital Songyuan City China Department of Anatomy School of Medicine Yanbian University Yanji City China Department of Dermatology Yanbian University Hospital Yanji City China

Objective: In vitro comparison of chondrogenic differentiation ability of bone marrow extracted mesenchymal stem cells (MSCs) and buffy coat. Methods: MSCs of New Zealand white rabbits were cultured in vitro and adherent cells were passaged. The cells were inoculated on polyglycolic acid (PGA) scaffold (3 mm in diameter and 2 mm in height) liquid for 21 days. Under the same conditions, without subculture, buffy coat was directly inoculated into the cell scaffold. The degree of chondrogenic differentiation was compared by Safranin-Ostaining, histological scoring and biochemical functional detection. Results: The chondrogenic differentiation ability of the buffy coat group was better than that of the MSC group. Safranin-Ostain was stronger in buffy coat group than in MSC group. The Bern Score was also higher in the buffy coat group than in the MSC group and the total amount of sulfated glycosaminoglycans (GAGs) in buffy coat group was higher than that in MSC group. Conclusion: The chondrogenic differentiation ability of buffy coat is higher than that of MSC. Through this result, it was found buffy coat can also differentiate into sub-cartilage without stimulating growth factor. Thus, buffy coat can make a great source in artificial cartilage engineering.

关键词： Chondrogenic buffy Coat Mesenchymal Stem Cells

The Application Prospect Autologous Bone Marrow Extract buffy Coat in Cartilage Tissue Engineering: A Mini-Review

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Journal of Biosciences and Medicines 2019年第12期7卷 9-17页

作者： Zhiyu Li Zhongye Zhang Guo Song Shouyong Fu Longhao Jin Yanqun Liu Department of Orthopedic Surgery Yanbian University Hospital Yanji China Department of Orthopedic Surgery Songyuan Central Hospital Songyuan China

Cartilage injury is an important cause of osteoarthritis;however, there is no effective method of cartilage regeneration at present. Therefore, it would be of great value to explore and develop new methods of cartilage regeneration. In recent years, it has become a hot topic on repairing damaged cartilage by using tissue engineered cartilage domestic and abroad. It’s the key factor to success for getting cell source which is effective, applicable and easy to obtain in the technology of cartilage tissue engineering filed. This article summarizes the research progress of bone marrow extract buffy coat in cartilage tissue engineering.

关键词： buffy Coat Cartilage Tissue Engineering

Passive gravitational sedimentation of peripheral blood increases the sensitivity of microscopic detection of malaria

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Asian Pacific Journal of Tropical Medicine 2013年第7期6卷 552-555页

作者： Richard Davis Trenden Flanigan Eric Wilson Department of Microbiology and Molecular Biology.Brigham Young University ProvoUtah 84602USA Department of Microbiology and Molecular Biology Brigham Young UniversityProvoUtah 84602USA

Objective:To determine if passive gravitational sedimentation of blood samples,followed by buffy coat thin smear preparation could increase the sensilivitv of malaria diagnosis when compared to conventional thin smear preparation without the additional cost of centrifuges or molecular *** Blood samples were collected from 205 patients,Each patient sample was analyzed using all three methods of sample ***:buffy coal analysis of centrifuged blood samples greatly increased the sensilivitv of malaria diagnosis when compared to standard thin smear *** between mechanically centrifuged samples and gravitationally sedimented samples showed equal improvement in sensitivity when compared to standard thin smear ***:Passive gravitational sedimentation of red blood cells followed by buffy coat analysis dramatically improves the sensitivity of malaria diagnosis without the additional costs associated with centrifugation.

关键词： Malaria buffy coat Sedimentation Thin smear

Bovine trypanosomosis: A threat to cattle production in Chena district, southwest Ethiopia

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Open Journal of Animal Sciences 2012年第4期2卷 287-291页

作者： Bizuayehu Alemayehu Basaznew Bogale Tewodros Fentahun Mersha Chanie Departement of Basic Veterinary Science Faculty of Veterinary Medicine University of Gondar Gondar Ethiopia Departement of Veterinary Paraclinical Studies Faculty of Veterinary Medicine University of Gondar Gondar Ethiopia

A cross-sectional study to determine the prevalence of bovine trypanosomosis was carried out in Chena district, Kefa zone, southwest Ethiopia from September 2010 to January 2011. Blood samples were collected from 391 randomly selected local (zebu) breed cattle in three representative peasant associations (PAs). The buffy coat and Giemsa stained thin blood films examination techniques were used for parasite detection and identification. The packed cell volume (PCV) estimation was also conducted. From a total of 391 cattle, 6.9% (n = 27) of the animals were found positive for trypanosome infection. The trypanosome species observed across the study animals were T. congolense (4.89%), T. vivax (1.54%), and T. b. brucei (0.51%) as single infections. The infection rate of T. congolense and T. b. brucei varied significantly (P 0.05) in infection rate was found between male (7.79%) and female (5.62%) animals. The prevalence was 2.24% and 8.29% in young and adult cattle, respectively with significant difference (P < 0.05) between them. The prevalence rate in good, medium and poor body conditioned animals were 7.28%, 0.78% and 13.39%, respectively with a statistical significant difference (P < 0.05) among them. The mean PCV of the infected animals (17.56%) appeared significantly (P < 0.05) lower than the non-infected (25.4%). Even if, the present study indicated a low prevalence of bovine trypanosomosis in the study areas, its impact on production and potential transmission to others should not be neglected.

关键词： Bovine buffy Coat Chena PCV Prevalence Trypanosomosis