Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat <i>in Vitro</i>

作     者：Longhao Jin Zhongye Zhang Yanqun Liu Kumar Gurung Yongjun Jin Yuanming He Yu Jin Zhehu Jin 

作者机构：Department of Orthopedic Surgery Yanbian University Hospital Yanji City China Department of Orthopedic Surgery Songyuan Central Hospital Songyuan City China Department of Anatomy School of Medicine Yanbian University Yanji City China Department of Dermatology Yanbian University Hospital Yanji City China 

出 版 物：《Journal of Biosciences and Medicines》 (生物科学与医学（英文）)

年 卷 期：2018年第6卷第3期

页      面：86-94页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Chondrogenic Buffy Coat Mesenchymal Stem Cells 

摘      要：Objective: In vitro comparison of chondrogenic differentiation ability of bone marrow extracted mesenchymal stem cells (MSCs) and buffy coat. Methods: MSCs of New Zealand white rabbits were cultured in vitro and adherent cells were passaged. The cells were inoculated on polyglycolic acid (PGA) scaffold (3 mm in diameter and 2 mm in height) liquid for 21 days. Under the same conditions, without subculture, buffy coat was directly inoculated into the cell scaffold. The degree of chondrogenic differentiation was compared by Safranin-Ostaining, histological scoring and biochemical functional detection. Results: The chondrogenic differentiation ability of the buffy coat group was better than that of the MSC group. Safranin-Ostain was stronger in buffy coat group than in MSC group. The Bern Score was also higher in the buffy coat group than in the MSC group and the total amount of sulfated glycosaminoglycans (GAGs) in buffy coat group was higher than that in MSC group. Conclusion: The chondrogenic differentiation ability of buffy coat is higher than that of MSC. Through this result, it was found buffy coat can also differentiate into sub-cartilage without stimulating growth factor. Thus, buffy coat can make a great source in artificial cartilage engineering.