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Journal of Chinese Pharmaceutical Sciences 2008年第4期17卷 291-296页

作者：刘立婷齐宪荣北京大学药学院药剂学系北京100191

Cationic polyethylenimine （PEI） with dextran fluorescein anionic （DFA） or oligodeoxynucleotide （ODN） could form polyelectrolyte complex by self-assembly as a gene delivery vector. This study was designed to investigate the effects on pharmaceutical characteristics and cell uptake PEI after a long-circulation modification with poly（ethylene glycol）（PEG）. DFA or ODN reacted with PEI or PEI-PEG to form polyelectrolyte complexes. Surface characters of these complexes and the retardation of ODN by PEI and PEI-PEG were evaluated. The uptake rates of DFA/PEI and DFA/PEI-PEG complexes by MCF-7 cells were evaluated by flow cytometry. Confocal laser scanning microscopy was utilized to visualize the internalization of these complexes. ODN/PEI complex showed the dependence of their size and ξ potential on the N/P ratio. ODN/PEI-PEG complex were much less affected by N/P ratio and their size was around 30 100 nm. PEI and PEI-PEG retarded ODN even at N/P ratio as low as 4, and complete retardation was found at N/P ratio of 8. The uptake rate by MCF-7 cells was direct correlated to the DFA concentration and incubation time, and the uptake rate could exceed 99% under the selected condition. The results in this study showed that PEI self-assembly polyelectrolyte complex after stealth or long circulation modification may increase the ability as a gene vector to delivery genes into cells.

关键词： Polyelectrolyte complex Polyethylenimine Uptake rate Self-assembly breast cancer cell

Effect of Cytokine on the Expression of Sodium Iodide Symporter Gene in breast cancer cell

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Journal of Nanjing Medical University 2004年第3期18卷 133-136页

作者： JIAYue LIUChao TANGWei LIUCui-ping QINYou-wen YUANQing-xing LIQian MAOXiao-dong DIFu-song DepartmentofEndocrinology theFirstAffiliatedHospitalofNanjingMedicalUniversityNanjing210029P.R.China

Objective: To investigate the effect of cytokines (TNF-α,IFN-γ and IL-6) on the expression of sodium-iodide symporter(NIS)gene in breast cancer cell(MCF-7). Methods:The breast cancer cell was cultureds with negative control culture or culture with different concentrations of cytokines for 72 *** gene mRNA in breast cancer cells cultured was determined by reverse transcriptase-polymerase chain reaction(RT-PCR). Results:Expression of sodium-iodide symporter mRNA can be found decreasing along with increasing the concentration of cytokine dose-dependently. Conclusion: Cytokine may play a role in iodide-uptake modulating in breast cancer cells by their effect on NIS gene expression.

关键词： sodium-iodide symporter breast cancer cell cytokine

Macrophage migration inhibitory factor promotes breast cancer cell proliferation and metastasis via AMPK pathway

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Macrophage migration inhibitory factor promotes breast cance...

The intra-herbal network effect of germacrone,curdione,and furanodiene combination on breast cancer cell proliferation

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Minjie Wang Yulong Bao Li Wang Lin Shi Fen Yun Xia Liu Yongxia Chen Yongfeng Jia School of Basic Medical Sciences Inner Mongolia Medical University Inner Mongolia cancer Hospital

Background: Macrophage migration inhibitory factor(MIF) is a pleiotropic cytokine with crucial roles in cell proliferation, tumorigenesis, angiogenesis and represents a target gene for therapy in the treatment of breast cancer. AMP-activated protein kinase(AMPK) is a nutrient-andmetabolic stress-sensing enzyme activated by the tumor suppressor kinase. The effect and relationship between MIF and AMPK on breast carcinoma cells is elusive. Our aim is to investigate the effect and mechanism of MIF and AMPK on proliferation and metastasis of breast cancer cells. Methods: MCF-7 breast carcinoma cells were used to investigate effects of MIF and AMPK. MIF levels was determined by Elisa kit. Genetic and protein expression of AMPK, MIF, LKB1 were quantified by quantitative polymerase chain reaction and Western Blotting. cell proliferation was studied by BrdU *** adhesion by Matrigel adhesion assay, and cell invasion by migration assay through Matrigel-coated filters using the Transwell system. Results: In this study, we demonstrated that under hypoxia microenvironment, MIF levels from MCF-7 breast cancer cells was increased. mRNA expression of MIF was up-regulated as well as the MIF protein expression. MIF up-regulating also results in inhibition of AMPK activity, and the concurrent and phosphorylation of AMPK were decreased under hypoxia conditions. LKB1, upstream gene of AMPK, was also inhibited. Ultimately,MIF up-regulating induced cell proliferation and adhesion of MCF-7 breast carcinoma ***: We conclude that under hypoxia conditions, MIF inhibit AMPK activation, induce proliferation and metastasis on MCF-7 breast carcinoma cells. MIF-AMPK pathway may play a pivotal role in the treatment of breast cancer.

关键词： Macrophage migration inhibitory factor AMP-activated protein kinase breast cancer cell Proliferation Metastasis

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The intra-herbal network effect of germacrone,curdione,and f...

Activation of Rac1-PI3K/Akt is required for epidermal growth factorinduced PAK1 activation and cell migration in MDA-MB-231 breast cancer cells

中国药理学会第十一届全国化疗药理学术研讨会

作者： Xiuping Chen (State Key Laboratory of Quality Research in Chinese Medicine,Macau,China Institute of Chinese Medical Sciences,University of Macau,Macau,China )

Traditional Chinese medicine(TCM) has been considered as "Hard to swallow" due to limited rational mechanism of action for most of its *** efforts and attempts have been made to dissect the action mode of TCM in recent years,which provided certain evidence for the inter-herbal interactions in TCM ***,the intra-herbal interactions have been largely neglected as each TCM herb containing dozens of components was replaced by a single pure compound in most *** present study,we asked,whether there are interactions among different components from a single *** effect of three main sesquiterpenes(germacrone(A),curdione(B),furanodiene(C)) isolated from a common TCM herb called WenEZhu(Curcuma Wenyujin *** et C Ling) on MDA-MB-231 and MCF-7 breast cancer cells proliferation alone or in combination with a fixed-dose-combination has been *** significantly inhibit cancer cells proliferation while germacrone and curdione showed no *** enhanced furanodiene’ s anti-proliferative *** showed no effect on furanodiene’ s anti-proliferative effect but partly reverse the anti-proliferative effect of germacrone and furanodiene *** morphological and mitochondrial membrane potential(△Ψm) changes showed similar ***,they demonstrated complicated interaction on the protein expressions of apoptotic-related proteins and key signaling transduction *** herb might be a network system with complex and unpredictable interactions among different *** intra-herb interactions should be taken into consideration when attempts are made to interpret the art of Chinese medical formulae or other similar recipes.

关键词： Curcuma *** C Ling proliferation breast cancer cell intra-herbal drug interactions Chinese medicine

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The Journal of Biomedical Research 2011年第4期25卷 237-245页

作者： Yu Yang Jun Du Zhenzhen Hu Jiaojing Liu Yinhui Tian Yichao Zhu Le Wang Luo Gu Department of Physiology Nanjing Medical University Nanjing Jiangsu 210029 China cancer Center Nanjing Medical University Nanjing Jiangsu 210029 China

Epidermal growth factor （EGF） may increase cell motility, an event implicated in cancer cell invasion and metastasis. However, the underlying mechanisms for EGF-induced cell motility remain elusive. In this study, we found that EGF treatment could activate Ras-related C3 botulinum toxin substrate 1 （Racl）, PI3K/Akt and p21- actived kinase （PAK1） along with cell migration. Ectopic expression of PAK1 K299R, a dominant negative PAK1 mutant, could largely abolish EGF-induced cell migration. Blocking PI3K/Akt signalling with LY294002 or Akt siRNA remarkably inhibited both EGF-induced PAK1 activation and cell migration. Furthermore, expression of dominant-negative Racl （T17N） could largely block EGF-induced PI3K/Akt-PAK1 activation and cell migration. Interestingly, EGF could induce a significant production of ROS, and N-acetyl-L-cysteine, a scavenger of ROS which abolished the EGF-induced ROS generation, cell migration, as well as activation of PI3K/Akt and PAK, but not Racl. Our study demonstrated that EGF-induced cell migration involves a cascade of signalling events, including activation of Racl, generation of ROS and subsequent activation of PI3K/Akt and PAK1.

关键词： breast cancer cell epidermal growth factor migration Ras-related C3 botulinum toxin substrate 1(Rac1) PI3K/Akt p21-actived kinase (PAK1)

Activin Receptor-like Kinase 7 Induces Apoptosis via Bax Family,Xiap and cell Cyclin Proteins in breast cancer cells

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Activin Receptor-like Kinase 7 Induces Apoptosis via Bax Fam...

Regulation of cell morphology and viability using anodic aluminum oxide with custom-tailored structural parameters

第九届西南三省一市生物化学与分子生物学学术交流会

作者：杨牧周红汪新艳电子科技大学生命科学与技术学院

In present study,the signal pathways involved in breast cancer cell apoptosis induced by activin receptor-like kinase 7 (ALK-7) were ***,the mRNAs of ALK-7,and its downstream effectors,Smad 2/3/4,were studied in human... 详细信息

关键词： activin receptor-like kinase 7 breast cancer cell apoptosis signal pathway

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Frontiers of Materials Science 2022年第4期16卷 15-26页

作者： Zhiying ZHANG Ting LIU Juan LI Yiyan GUO Ruiqing LIANG Jiangbo LU Runguang SUN Jun DONG School of Physics and Information Technology Shaanxi Normal UniversityXi'an 710119China Department of Orthopaedics Second Affiliated Hospital of Xi'an Jiaotong UniversityXi'an 710004China

Anodic aluminum oxide(AAO)with independently controlled period,porosity,and height is used as the model surface to study the single structural parameter effect on breast cancer cell behaviors,including cell polarity and cell *** is found that the quantity of multipolar cells and cell viability increases as the nanodent period increases from 100 to 300 nm,while the number of bipolar cells has almost no change until there is a dramatic decrease as the period increases to 3oo *** anodizing nanodents into nanopores,the numbers of both bipolar cells and the cell viability increase significantly with the porosity ***,as the porosity further increases and the nanopore changes into a nanocone pillar,most of the cells become nonpolar spheres and the cell viability *** the height of the nanocone pillar has little effect on the cell polarity;the cell viability increases slightly with the increase of the nanocone pillar *** results reveal the influence of individual nanostructure parameters on the cell behavior,especially the cell polarity and the cell viability,which can help to design the surface to make the cell grow as desired.

关键词： nanostructure parameter breast cancer cell cell morphology cell polarity cell viability

Cyclin E Expression and Chemotherapeutic Sensitivity in breast cancer cells

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2006年第5期26卷 565-566页

作者：陈剑英王国斌 Department of General Surgery Union HospitalHuazhong University of Science and TechnologyWuhan 430022China

The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-β-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs （adriamycin, etc.）. Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.

关键词： cyclin E DNA damage RNA interference breast cancer cell

Transcriptomic analysis of human breast cancer cells reveals differentially expressed genes and related cellular functions and pathways in response to gold nanorods

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Biophysics Reports 2015年第2期1卷 106-114页

作者： Teng Zhou Yipeng Du Taotao Wei National Laboratory of Biomacromolecules Institute of Biophysics Chinese Academy of Sciences

breast cancer is the leading cause of cancer deaths in women. Recent advances in nanomedicine have shown that gold nanorods(Au NRs), as multifunctional drug delivery and photothermal therapeutic agents, have potential for use in cancer therapy. However, the effect of Au NRs on the transcriptome of breast cancer cells is unknown. In the present study, cells of the triple-negative human breast cancer cell line MDA-MB-231,which has high metastatic activity, were treated with Au NRs for transcriptomic analysis using RNA-seq technology. In total, 3126 genes were found to be up-regulated and 3558 genes were found to be downregulated in Au NR-treated MDA-MB-231 cells. These differentially expressed genes presumably take part in multiple biological pathways, including glycolysis and regulation of the actin cytoskeleton, and impact a variety of cellular functions, including chemoattractant activity. The distinct gene expression profile of MDA-MB-231 cells treated with Au NRs provides a foundation for further screening and validation of important genes involved in the interaction between Au NRs and MDA-MB-231 cells.

关键词： breast cancer cell Glycolysis Gold nanorod RNA-seq technology Regulation of actin cytoskeleton

Apoptosis of T-47D cells Induced by Cinobufacini via a Caspase-3-dependent Manner

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Chemical Research in Chinese Universities 2014年第1期30卷 108-113页

作者： WANG Weiqi SHI Aiping FAN Zhimin Department of breast Surgery the First Hospital of Jilin University Changchun 130021 P. R. China

To investigate the mechanism of the anti-tumor activity of cinobufacini on the breast cancer cell line T-47D,the inhibitory effect of cinobufacini on the proliferation of T-47D was detected via MTT assay and the morphological changes of T-47D and HBL-100 cells caused by cinobufacini were observed with an inverted *** apoptosis and cell cycle stages were detected by flow cytometry *** effects of cinobufacini on the expression of active-form and pro-form of caspase-3 were assessed by Western blot *** dramatically inhibited T-47D proliferation in a dose-and time-dependent *** found that more than 20％ of T-47D cells were killed after treatment with 20 mg/mL cinobufacini for 24 h in *** 6 d of treatment with 20 mg/mL cinobufacini,the cell survival rate decreased by more than 40％.Flow cytometric analysis demonstrated that cinobufacini induced significant apoptosis and changes of the cell cycle distribution of T-47D *** used breast cell line HBL-100 as the control,the above experiments except cell cycle analysis showed that cinobufacini more obviously induced the apoptosis of T-47D cells than that of HBL-100 *** blot analysis confirmed the protein expression of active caspase-3 increased with increasing the dose of *** results indicate that cinobufacini induces the apoptosis of T-47D cells via the up-regulation of caspase-3.

关键词： breast cancer cell Cinobufacini Apoptosis Caspase-3