Apoptosis of T-47D Cells Induced by Cinobufacini via a Caspase-3-dependent Manner
Apoptosis of T-47D Cells Induced by Cinobufacini via a Caspase-3-dependent Manner作者机构：Department of Breast Surgery the First Hospital of Jilin University Changchun 130021 P. R. China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2014年第30卷第1期
页 面：108-113页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 071005[理学-微生物学] 090102[农学-作物遗传育种] 10[医学]
基 金：Supported by the National Natural Science Foundation of China(No.30300336)
主 题：Breast cancer cell Cinobufacini Apoptosis Caspase-3
摘 要：To investigate the mechanism of the anti-tumor activity of cinobufacini on the breast cancer cell line T-47D,the inhibitory effect of cinobufacini on the proliferation of T-47D was detected via MTT assay and the morphological changes of T-47D and HBL-100 cells caused by cinobufacini were observed with an inverted *** apoptosis and cell cycle stages were detected by flow cytometry *** effects of cinobufacini on the expression of active-form and pro-form of caspase-3 were assessed by Western blot *** dramatically inhibited T-47D proliferation in a dose-and time-dependent *** found that more than 20％ of T-47D cells were killed after treatment with 20 mg/mL cinobufacini for 24 h in *** 6 d of treatment with 20 mg/mL cinobufacini,the cell survival rate decreased by more than 40％.Flow cytometric analysis demonstrated that cinobufacini induced significant apoptosis and changes of the cell cycle distribution of T-47D *** used breast cell line HBL-100 as the control,the above experiments except cell cycle analysis showed that cinobufacini more obviously induced the apoptosis of T-47D cells than that of HBL-100 *** blot analysis confirmed the protein expression of active caspase-3 increased with increasing the dose of *** results indicate that cinobufacini induces the apoptosis of T-47D cells via the up-regulation of caspase-3.
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