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bovine serum albumin:An efficient and green biocatalyst for the one-pot four-component synthesis of pyrano[2,3-c]pyrazoles

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Chinese Journal of Catalysis 2016年第9期37卷 1461-1468页

作者： Xingtian Huang Zhipeng Li Dongyang Wang Yiqun Li College of Life Science and Technology Jinan University Guangzhou 510632 Guangdong China Department of Chemistry Jinan University Guangzh ou 510632 Guangdong China

The use of biocatalysts is attracting an increasing amount of attention in chemical ***,we have shown that bovine serum albumin（BSA）,a ubiquitous,inexpensive,non-enzymatic transport protein,can serve as an efficient,retrievable catalyst in the one-pot four-component reaction of aryl aldehydes,malononitrile,hydrazine hydrate,and ethyl acetoacetate for the synthesis of pyrano[2,3-c]pyrazoles under mild reaction *** BSA biocatalyst also displayed a high catalytic affinity for acyclic/cyclic ketones to yield the corresponding pyrano[2,3-c]pyrazoles or their spirocyclic *** BSA could be used for at least five cycles without serious loss of catalytic *** novel,efficient protocol has the merits of high yield,operational simplicity,and a relatively benign environmental ***,the method extends the promiscuity of BSA as a biocatalyst.

关键词： bovine serum albumin Biocatalyst Multicomponent reaction Pyrano[2 3-c]pyrazole Biocatalytic promiscuity

Studies on Reaction Mechanism Between Sparfloxacin and bovine serum albumin

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Chemical Research in Chinese Universities 2004年第1期20卷 81-87页

作者： GUOMing ZOUJian-wei YIPing-gui SHANGZhi-cai HUGui-xiang YUQing-sen DepartmentofChemistry ZhejiangUniversityHangzhou310027P.R.China TarimUniversityofAgriculturalReclamation

The binding of sparfloxacin and bovine serum albumin(BSA) in aqueous solution was studied by means of fluorescence and absorbance spectra, and the interactions influenced by Fe 3+ and Cu 2+ were explored. Based on the Scatchard′s site binding model and fluorescence quenching, practical formulas for a small molecule ligand attaching to a bio-macromolecule are proposed. The binding parameters were measured according to the suggested models, and the binding distance, the transfer efficiency of energy between sparfloxacin and BSA were obtained in view of the Frster theory of non-radiation energy transfer. The effect of sparfloxacin on the conformation of BSA was analyzed by means of synchronous fluorescence spectroscopy.

关键词： Sparfloxacin bovine serum albumin Fluorescence spectroscopy

Spectroscopic Studies on the Binding of Kaempferol-3,7-α-L- rhamnopyranoside to bovine serum albumin

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Chinese Journal of Chemistry 2012年第2期30卷 438-444页

作者： Yao,Di Yu,Jing Pan,Yingming Huang,Fuping Bian,Hedong Yu,Qing Liang,Hong Chen,Zhenfeng Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University) Ministry of Education of ChinaGuilinGuangxi 541004China

The binding of kaempferol-3,7-α-L-rhamnopyranoside （KRR） with bovine serum albumin （BSA） was investi- gated by different spectroscopic methods under simulative physiological conditions. Analysis of fluorescence quenching data of BSA by KRR at different temperatures using Stern-Volmer methods revealed the formation of a ground state KRR-BSA complex with moderate binding constant of the order 10^4 Lomol-1. The existence of some metal ions could weaken the binding of KRR on BSA. The changes in the van＇t Hoff enthalpy （△H0） and entropy （△S0） of the interaction were estimated to be --26.53 ***-1 and 3.33 ***-l.K-1 and both hydrophobic and electrostatic forces contributed to stabilizing the BSA-KRR complex. According to the F6ster theory of non-radiation energy transfer, the distance r between the donor （BSA） and the acceptor （KRR） was obtained （r= 2.83 nm）. Site marker competitive experiments showed that KRR could bind to Site I of BSA. In addition, synchronous fluorescence, UV-Vis absorption and circular dichroism （CD） results indicated that the KRR binding could cause conformational changes of BSA.

关键词： kaempferol-3,7-α-L-rhamnopyranoside bovine serum albumin UV-Vis spectroscopy fluorescence spectroscopy circular dichroism

Investigation of the topological shape of bovine serum albumin in solution by small-angle x-ray scattering at Beijing synchrotron radiation facility

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Chinese Physics B 2008年第12期17卷 4574-4579页

作者：董淑强李丽琴刘鹏董宇辉陈熙萌 Nuclear Science and Technology Department of Lanzhou University Institute of High Energy Physics Chinese Academy of Sciences

This paper reports that at a newly constructed small-angle x-ray scattering station of Beijing Synchrotron Radia- tion Facility, the topological shape of ligand-free bovine serum albumin in solution has been investigated. An appropriate scattering curve is obtained and the calculated value of the gyration radius is 31.2~=t=0.25 ~_ （11=0.1 nm） which is co- incident with other ones＇ results. It finds that the low-resolution structure models obtained by making use of ab initio reconstruction methods are fitting the crystal structure of human serum albumin very well. All of these results perform the potential of the beamline to apply to structural biology studies. The characteristics, the defects, and the improving measures of the station in future are also discussed.

关键词： small-angle x-ray scattering ab initio methods bovine serum albumin Beijing synchrotron radiation facility

Preparation of MnO2-impregnated carbon-coated Fe3O4 nanocomposites and their application for bovine serum albumin adsorption

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Rare Metals 2020年第10期39卷 1151-1158页

作者： Chol-Hwan Kim Ze-Fei Zhang Lin-Shan Wang Ting Sun College of Sciences Northeastern UniversityShenyang 110004China

MnO2-impregnated carbon-coated Fe3 O4(Fe3O4/C/MnO2)nanocomposites with a good core-shell structure were prepared by direct oxidation of carbon-coated Fe3 O4(Fe3O4/C)microspheres with KMnO4 in alkaline solution and applied to adsorb bovine serum albumin(BSA).X-ray diffraction(XRD),transmission electron microscope(TEM),Fourier transform infrared spectrometer(FTIR),vibrating sample magnetometer(VSM)and thermogravimetric analyzer(TGA)tests show that Fe3O4/C microspheres were newly functionalized via the oxidation by ***3O4/C/MnO2 nanocomposites exhibit a higher adsorption capacity for BSA than Fe3O4/C microspheres and the maximum adsorption of BSA on them occurs at pH 4.7,which is the isoelectric point of *** isotherm model describes the adsorption of BSA better than Freundlich model and Temkin model,and the kinetics data fit well with the pseudo-second-order model.

关键词： Fe3O4/C/MnO2 nanocomposites bovine serum albumin Adsorption

bovine serum albumin Detection by Graphene Oxide Coated Long-Period Fiber Grating

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Photonic Sensors 2022年第3期12卷 48-57页

作者： Ruiduo WANG Hao WU Mei QI Jing HAN Zhaoyu REN The State Key Laboratory of Photoelectric Technology and Functional Materials International Collaborative Center on Photoelectric Technology and Nano Functional MaterialsInstitute of Photonics&Photon TechnologyNorthwest UniversityXi’an 710127China The State Key Laboratory of Transient Optics and Photonics Xi’an Institute of Optics and Precision MechanicsChinese Academy of SciencesXi’an 710119China The School of Information Science and Technology Northwest UniversityXi’an 710127China

A biosensor for bovine serum albumin(BSA)detection by graphene oxide(GO)functionalized micro-taped long-period fiber grating(GMLPG)was *** amide bond connected between the GO and BSA enabled the BSA to attach onto the fiber surface,which changed the effective refractive index of the cladding mode and characterized the concentration of the *** real-time monitoring system demonstrated a sensing sensitivity of 1.263 nm/(mg/mL)and a detection limit of 0.043 mg/***,it illustrated superior measurement performance of higher sensitivity in the presence of glucose and urea as the interference,which showed static sensitivities of~1.476 nm/(mg/mL)and 1.504 nm/(mg/mL),*** proposed GMLPG demonstrated a great potential for being employed as a sensor for biomedical and biochemical applications.

关键词： Graphene oxide bovine serum albumin biosensor

Interaction of epicatechin with bovine serum albumin using fluorescence quenching combined with chemometrics

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Science China Chemistry 2014年第5期57卷 748-754页

作者： ZHAI Min WU HaiLong ZHANG ShuRong ZHANG XiHua SUN YanMei YU RuQin State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

The interaction between BSA and epicatechin was studied using fluorescence quenching titrations combined with trilinear decomposition method and excitation-emission matrix(EEM)*** resolved spectra were highly similar with the actual ones which indicated that the resolved results were *** relevant parameters of the binding process were obtained by quantifying each substance in the complicated mixtures in *** quenching was static quenching,epicatechin had a weak interaction with BSA and the binding site was *** total concentration and the free concentration of quenchers had different effect on the *** results demonstrated that the method exploited in this article is a useful tool to investigate complicated interactions,avoiding complicated pretreatment and simplify experimental procedure.

关键词： epicatechin bovine serum albumin three-dimensional fluorescence quenching trilinear decomposition method in situinterpretation of binding parameters

Study on Binding Reaction between Flucytosine and bovine serum albumin

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Chinese Journal of Chemistry 2005年第9期23卷 1151-1156页

作者：颜承农张华新梅平刘义 College of Chemistry and Environmental Engineering Yangtze University Jingzhou Hubei 434020 China College of Chemistry and Molecular Sciences Wuhan University Wuhan Hubei 430072 China

The binding of flucytosine to bovine serum albumin （BSA） was studied by means of fluorescence and absorption spectra under the conditions of simulant clay physiology. It showed a powerful ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data by Stem-Volmer equation and Lineweaver-Burk double-reciprocal equation, it was found that they matched the latter better and so they belonged to static quenching. The binding constant was calculated to be 5.710 × 10^3 L·mol^-1 at 297 K. The binding locality was a distance 2.49 nm away from tryptophan residue-212 based on Foster＇s non-radiation energy transfer mechanism. The binding power is mainly the hydrogen bond and van der Waals force according to the thermodynamic parameters. The information of BSA conformation was acquired by synchronous fluorescence spectrum and three-dimensional fluorescence spectrum.

关键词： flucytosine bovine serum albumin fluorescence spectroscopy three-dimensional fluorescence spectrum thermodynamic parameter

Preparation of （2E）-3-（4＇-Halophenyl）prop-2-enoyl Sulfachlorpyridazine Sodium Salts and Their Interaction with bovine serum albumin by Fluorescence Spectroscopy

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Chemical Research in Chinese Universities 2013年第5期29卷 854-860页

作者： DU Chuan-rong LUO Xuan WEI Jin-rui HE Ting-ting ZHENG Xiao-yu LIN Cui-wu School of Chemistry and Chemical Engineering Guangxi University Nanning 530004 P. R. China

Three （2E）-3-（4＇-halophenyl）prop-2-enoyl sulfachlorpyridazine sodium salts（XPSCA） were synthesized. Their chemical structures were confirmed by IH NMR and 13C NMR, electrospray ionization mass spectrometry （ESI-MS）, and infrared（IR） spectroscopy. The interactions between XPSCA and bovine serum albumin（BSA） were investigated under imitated physiological condition by fluorescence quenching technique and UV-Vis absorption spectroscopy according to the Stern-Volmer equation. The results from the emission quenching at different tempera- tures indicate that the quenching mechanism of serum albumin by XPSCA was static quenching mechanism at low XPSCA concentrations or a combined quenching（static and dynamic） mechanism at higher XPSCA concentrations. At different temperatures, the binding constant and the binding sites of XPSCA with BSA were investigated, and the distances were evaluated according to F6rster non-radiative resonance energy transfer theory. The thermodynamic parameters were calculated according to van＇t Hoff equation, which implies that both van der Waals interaction and hydrogen bond played major roles in stabilizing the XPSCA-BSA complexes, whereas hydrophobic interactions were secondary. Moreover, the conformational changes in BSA were analyzed by synchronous fluorescence spectra.

关键词： Sulfachlorpyridazine sodium bovine serum albumin Halo-cinnamic acid Fluorescence spectroscopy