Bovine Serum Albumin Loaded Solid Lipid Nanoparticles Prepared by Double Emulsion Method

作     者：LI Zhen LI Xin-wei ZHENG Li-qiang LIN Xiao-hong GENG Fei YU Li 

作者机构：Key Laboratory of Colloid and Interface Chemistry Ministry of Education Shandong University Jinan 250100 P. R. China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2010年第26卷第1期

页      面：136-141页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：Supported by the National Natural Scientific Foundation of China(No.50472069) the Key Scientific Project from the Chinese Education Ministry(No.106100) 

主　　题：Solid lipid nanoparticle Double emulsion Bovine serum albumin Drug model 

摘      要：Solid lipid nanoparticles loaded with bovine serum albumin（BSA） were prepared by a double emulsion method. As the mass fraction of the model drug BSA increased from 0 to 15%, the particle size gradually increased. The physical stability of the nanoparticles was investigated by zeta potential measurement and they were shown to be quite stable. Fluorescence spectroscopy confirmed that the loaded position of BSA was on the interface between the inner aqueous phase and the solid lipid phase. Both Fourier-transform infrared spectroscopy and circular dichroism spectra indicate that BSA in the nanoparticles was not destroyed, but the secondary structure was disrupted slightly.