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Erxian decoction potentially prevents postmenopausal osteoporosis by modulating miR-335: a study based on bioinformatics analysis and preliminary clinical case validation

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Traditional Medicine Research 2023年第6期8卷 1-8页

作者： Hao-Qiang Huang Ye Feng Xiao-Feng Shen Yu Zhou Li Qin Feng Xu Qing Wang Department of Orthopaedics Kunshan Hospital of Traditional Chinese MedicineKunshan 215300China School of Stomatology Xuzhou Medical UniversityXuzhou 221000China Department of Orthopedics Suzhou Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese MedicineSuzhou 215100China Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism School of PharmacyEast China University of Science and TechnologyShanghai 200237China.

Background:miRNAs are closely related to bone *** have shown that Erxian decoction can improve bone metabolism,possibly achieving this regulatory effect through miRNA *** was used to predict the miRNA targets of Erxian decoction for the treatment of postmenopausal osteoporosis,and the results were validated by clinical ***:In this study,we identified possible targets of Erxian decoction in osteoporosis by means of network pharmacological analysis and bioinformatic *** cases of postmenopausal osteoporosis with kidney Yin and Yang deficiency(In traditional Chinese medicine,kidney Yin nourishes and moistens the tissues of the internal organs of the body,while kidney Yang promotes and warms the tissues of the internal organs of the body.)were treated with Erxian decoction for four weeks,and serum bone metabolism indices(P1NP,osteocalcin,andβ-CTX)and miRNA-335-5p expression were measured before and after ***:The constructed miRNA postmenopausal osteoporosis related gene network of the effective compound of the Erxian decoction has 296 points and 981 *** 39 postmenopausal osteoporosis related genes regulated by miRNA-335-5p were enriched in ossification,while the signaling pathways were enriched in rheumatoid arthritis,the Toll signaling pathway,the HIF-1 signaling pathway,and the MAPK signaling *** taking Erxian decoction,the expression of the serum bone formation index(P1NP,osteocalcin)and miRNA-335-5p gene expression levels increased *** alterations in P1NP and osteocalcin were correlated with the changes in ***:Circulating miRNA-335-5p may serve as an important target of Erxian decoction in the treatment of postmenopausal *** effect of Erxian decoction on bone formation is significant,but the underlying mechanism requires further investigation.

关键词： Erxian decoction postmenopausal osteoporosis miRNA-335-5p bone formation bioinformatics clinical cases

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Dose-dependent tandem responses of osteoblasts during osteogenesis

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Medicine in Novel Technology and Devices 2023年第2期18卷 114-122页

作者： Yuwei Zhang Lizhen Wang Hongyan Kang Chia-Ying Lin Yubo Fan Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education Beijing Advanced Innovation Centre for Biomedical EngineeringSchool of Biological Science and Medical EngineeringBeihang UniversityBeijing100083China School of Engineering Medicine Beihang University100083BeijingChina Department of Biomedical Chemical&Environmental EngineeringUniversity of CincinnatiCincinnatiOH45267USA Department of Orthopaedic Surgery University of CincinnatiCincinnatiOH45267USA Department of Neurosurgery University of CincinnatiCincinnatiOH45267USA

Irisin,a myokine mainly secreted from contracted skeleton muscle,plays a profound role in bone formation and *** irisin has been revealed to elevate bone mass in vivo,details whether there is a dosedependent relationship between irisin and bone formation remain *** this study,we explored the dosedependent effects of irisin on osteoblast proliferation and *** results first demonstrated a remarkable increase in cell proliferation rate and viability in response to elevated concentrations of r-irisin,which was further enhanced over ***,this increase was subject to complex dose-response relationships as the proliferation-enhancing effects of r-irisin may have a saturation point between 10 ng/ml and 100 ng/***,we determined that 1,10,and 100 ng/ml r-irisin were able to upregulate the expression of osteogenic transcription factors(Runx2,Osx,and Atf4),as well as osteogenic markers(Alp,Col1a1 and Spp1),albeit without significant difference among these 3 ***,nutrient-depleted osteoblasts and those with standard culture showed distinct responses to higher doses of irisin regarding osteogenic *** investigation is required to uncover the molecular mechanisms underlying the observed tandem effects of irisin on osteogenesis.

关键词： Irisin Osteoblast Proliferation Differentiation bone formation

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Preparation,characterization and biological properties of a novel bone block composed of platelet rich fibrin and a deproteinized bovine bone mineral

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Fundamental Research 2022年第2期2卷 321-328页

作者： Mengge Feng Yulan Wang Yan Wei Xiaoxin Zhang Leyi Xiao Zijjian Gong Masako Fujioka-Kobayashi Anton Sculean Richard J Miron Scott Froum Yufeng Zhang The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei MOST)&Key Laboratory of Oral Biomedicine Ministry of Education School&Hospital of StomatologyWuhan University Department of Oral and Mxillfacial Surgery The Nippon Dental University School of Life Dentistry at TokyoTokyoJapan Department of Periodontology University of BernBernSwitzerland Clinical Assistant Professor State University of New York Story Brook Department of PeriodontologyPrivate Practice New YorkNew YorkUSA Medical Research Institute School of MedicineWuhan UniversityWuhan 430071China

Alveolar bone defects caused by tooth loss often lead to challenges in implant dentistry,with a need for development of optimal bone biomaterials to predictably rebuild these *** address this problem,we fabricated a novel bone block using platelet-rich fibrin(PRF)and Deproteinized Bovine bone Mineral(DBBM),and characterized their mechanical and biological *** bone block was prepared by mixing DBBM,Liquid-PRF,and Solid-PRF fragments in various combinations as follows:(1)BLOCK-1 made with Solid-PRF fragments+DBBM,(2)BLOCK-2 made with Liquid-PRF+DBBM,(3)BLOCK-3 made with Solid-PRF fragments+Liquid-PRF+*** time for solidification and the degradation properties were subsequently *** electron microscopy(SEM)and tensile tests were carried out to investigate the microstructure and mechanical properties of each *** bioactivity of the three groups towards osteoblast differentiation was also evaluated by culturing cells with the conditioned medium from each of the three groups including cell proliferation assay,cell migration assay,alkaline phosphatase(ALP)staining,and alizarin red staining(ARS),as well as by real-time PCR for genes encoding runt-related transcription factor 2(RUNX2),ALP,collagen type I alphal(COLIv41)and osteocalcin(OCN).BLOCK-3 made with Solid-PRF fragments+Liquid-PRF+DBBM had by far the fastest solidification period(over a 10-fold increase)as well as the most resistance to *** and tensile tests also revealed that the mechanical properties of BLOCK-3 were superior in strength when compared to all other groups and further induced the highest osteoblast migration and osteogenic differentiation confirmed by ALP,ARS and real-time *** bone blocks made through the combination of Solid-PRF fragments+Liquid-PRF+DBBM had the greatest mechanical and biological properties when compared to either used *** clinical studies are warranted to further support the clinical application of PRF bone blocks in bo

关键词： Blood Platelet-rich fibrin bone formation Dental implant Regeneration

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Exercise promotes osteogenic differentiation by activating the long non-coding RNA H19/microRNA-149 axis

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World Journal of Orthopedics 2024年第4期15卷 363-378页

作者： Xu-Chang Zhou Dong-Xue Wang Chun-Yu Zhang Ya-Jing Yang Ruo-Bing Zhao Sheng-Yao Liu Guo-Xin Ni School of Sport Medicine and Rehabilitation Beijing Sport UniversityBeijing 100084China Department of Acupuncture and Moxibustion Hubei University of Chinese MedicineWuhan 430065Hubei ProvinceChina Department of Spinal Surgery The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510260Guangdong ProvinceChina Department of Rehabilitation Medicine The First Affiliated Hospital of Xiamen UniversityXiamen 361003Fujian ProvinceChina

BACKGROUND Regular physical activity during childhood and adolescence is beneficial to bone development,as evidenced by the ability to increase bone density and peak bone mass by promoting bone *** To investigate the effects of exercise on bone formation in growing mice and to investigate the underlying *** 20 growing mice were randomly divided into two groups:Con group(control group,n=10)and Ex group(treadmill exercise group,n=10).Hematoxylin-eosin staining,immunohistochemistry,and micro-CT scanning were used to assess the bone formation-related indexes of the mouse *** analysis was used to find potential miRNAs targets of long non-coding RNA H19(lncRNA H19).RT-qPCR and Western Blot were used to confirm potential miRNA target genes of lncRNA H19 and the role of lncRNA H19 in promoting osteogenic *** Compared with the Con group,the expression of bone morphogenetic protein 2 was also significantly *** micro-CT results showed that 8 wk moderate-intensity treadmill exercise significantly increased bone mineral density,bone volume fraction,and the number of trabeculae,and decreased trabecular segregation in the femur of *** of lncRNA H19 significantly upregulated the expression of miR-149 and suppressed the expression of markers of osteogenic *** addition,knockdown of lncRNA H19 significantly downregulated the expression of autophagy markers,which is consistent with the results of autophagy-related protein changes detected in mouse femurs by *** Appropriate treadmill exercise can effectively stimulate bone formation and promote the increase of bone density and bone volume in growing mice,thus enhancing the peak bone mass of *** lncRNA H19/miR-149 axis plays an important regulatory role in osteogenic differentiation.

关键词： Exercise Osteogenic differentiation bone formation bone mesenchymal stem cells Autophagy

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Rapid tooth movement through distraction osteogenesis of the periodontal ligament in dogs

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Chinese Medical Journal 2008年第5期121卷 455-462页

作者： AI Hong XU Qing-feng LU Hong-fei MAI Zhi-hui AN Ai-qun LIU Guo-ping Department of Stomatology Affiliated Hospital Guangzhou Sun Yat-sen University Third Guangdong 510630 China

Background Animal models are needed for the study of rapid tooth movement into the extraction socket through distraction osteogenesis of the periodontal ligament. Methods Modified distraction devices were placed on eight dogs between the first and third mandibular premolars on the left sides; similar placement of traditional straight wise appliances on the right sides served as the control. The experimental distractors were activated （0.25 mm/d） twice a day and the control devices were activated （100 g） for two weeks with consolidation periods at weeks two, three, six, and ten. Two dogs were sacrificed at each consolidation time point; rates and patterns of tooth movement, loss of anchorage, and periapical films were evaluated, and the affected premolars and surrounding periodontal tissues were decalcified and examined histologically. General observations, X-ray periapical filming and histology examination were performed. Results Distal movement （（3.66±0.14） mm） measured two weeks after modified distraction exceeded that achieved using the traditional device （（1.15±0.21） mm; P 〈0.05）. Loss of anchorage was minimally averaged （0.34±0.06） mm and （0.32±0.07） mm in the experimental and control sides, respectively. By radiography, apical and lateral surface root resorptions on both sides were minimal. Alveolar bone lesions were never evident. Fibroblasts were enriched in periodontal ligaments and bone spicules formed actively along directions of distraction. Conclusions The canine model is suitable for the study of rapid tooth movement through distraction osteogenesis of the periodontal ligament. The technique accelerates tooth movement, periodontal remodeling, alveolar bone absorption, and may induce fibroblast formation, as compared to the traditional orthodontic method, without adversely affecting root absorption, bone loss, tooth mobility and anchorage loss.

关键词： animal model distraction osteogenesis periodontal ligament rapid tooth movement bone formation

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Differential bone metabolism and protein expression in mice fed a high-fat diet versus Daurian ground squirrels following natural pre-hibernation fattening

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第12期23卷 1042-1056页

作者： Xuli GAO Shenyang SHEN Qiaohua NIU Weilan MIAO Yuting HAN Ziwei HAO Ning AN Yingyu YANG Yu ZHANG Han ZHANG Kenneth BSTOREY Hui CHANG Shaanxi Key Laboratory for Animal Conservation Northwest UniversityXi'an 710069China Key Laboratory of Resource Biology and Biotechnology in Western China(College of Life Sciences Northwest University)Ministry of EducationXi'an 710069China Department of Biology Carleton UniversityON K1S 5B6Canada

This study compared the effects on bone metabolism and morphology of pathological obesity induced by excessive fat intake in a non-hibernator(mice)versus healthy obesity due to pre-hibernation fattening in a hibernator(ground squirrels).Kunming mice were fed a high-fat diet to provide a model of pathological obesity(OB group).Daurian ground squirrels fattened naturally in their pre-hibernation season(PRE group)were used as a healthy obesity ***-computed tomography(micro-CT)and three-point bending tests were used to determine the microstructure and mechanical properties of *** blots were used to analyze protein expression levels related to bone metabolism(Runt-related transcription factor 2(RunX2),osteocalcin(OCN),alkaline phosphatase(ALP),osteoprotegerin(OPG),receptor activator of nuclear factor-κB ligand(RANKL),cathepsin K,matrix metallopeptidase 9(MMP9),patched protein homolog 1(Ptch1),phosphorylatedβ-catenin(P-β-catenin),and glycogen synthase kinase-3β(GSK-3β)).Compared with controls,there was no obvious bone loss in the OB mice,and the stiffness of the femur was increased *** with summer active squirrels,bone formation was enhanced but the mechanical properties did not change in the PRE group *** OB mice,western blots showed significantly increased expression levels of all proteins except RunX2,OPG,and *** ground squirrels showed significantly increased expression of most proteins except OCN and Ptch1,which decreased significantly,and P-β-catenin and OPG,which did not *** conclusion,for non-hibernating mice,moderate obesity had a certain protective effect on bones,demonstrating two-way regulation,increasing both bone loss and bone *** pre-hibernating ground squirrels,the healthy obesity acquired before hibernation had a positive effect on the microstructure of bones,and also enhanced the expression levels of proteins related to bone formation,bone resorption,and Wnt signaling.

关键词： High-fat diet Pre-hibernation fattening bone formation bone loss Wnt signaling

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In Vivo Estimation of Osteogenesis by bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components

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Journal of Biomedical Science and Engineering 2016年第11期9卷 501-514页

作者： Masataka Yoshikawa Hideyuki Kakigi Ayano Miyamoto Sadaomi Sugimoto Keisuke Nakai Hideaki Ikenaga Takeshi Inamoto Hiroshi Maeda Department of Endodontics Osaka Dental University Osaka Japan

A bi-phasic scaffold consisting of a columnar formaldehyde-acetalized polyvinyl alcohol (PVF) sponge and a cylindrical porous hydroxyapatite (HA) with a hollow center was devised. Rat bone marrow cells (rBMCs) were seeded into the sponge placed in the hollow center of the cylindrical porous HA. The bi-phasic scaffold, a cylindrical porous HA and a PVF sponge separated from a bi-phasic scaffold after rBMC seeding, and a PVF sponge without rBMCs as a negative control, were implanted for 6 weeks into rat dorsal subcutaneous tissue. In each construct, bone formation was examined histologically and osteocalcin was measured immunochemically. bone formation was observed in the bi-phasic scaffold and also in the cylindrical porous HA isolated from the bi-phasic scaffold. A significant difference in the quantity of osteocalcin was observed between the bi-phasic scaffold and the isolated cylindrical porous HA. No bone formation was found in the isolated PVF sponge. The bi-phasic scaffold as an outer layer of the scaffold seemed to inhibit the outflow of rBMCs from the PVF sponge. This type of bi-phasic scaffold may have two specific characteristics: Attachment of cells both in PVF sponge and cylindrical porous HA.

关键词： Bi-Phasic Scaffold Hydroxyapatite Polyvinyl Alcohol Sponge bone formation In Vivo

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Integrins mediating bone signal transduction

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Journal of Chongqing University 2004年第1期3卷 55-60页

作者： HEChuanglong WANGYuanliang YANGLihua ZHANGJun KeyLaboratoryforBiomechanics＆TissueEngineeringUndertheMinistryofEducation ChongqingUniversityChongqing400030P.R.China BioengineeringCollege ChongqingUniversityChongqing400030P.R.China

Integrin-mediated adhesions play critical roles in diverse cell functions. Integrins offers a platform on which mechanical stimuli, cytoskeletal organization, biochemical signals can concentrate. Mechanical stimuli transmitted by integrins influence the cytoskeleton, in turn, the cytoskeleton influences cell adhesion via integrins, then cell adhesion results in a series of signal transduction cascades. In skeleton, integrins also have a key role for bone resoption by osteoclasts and reformation by osteoblasts. In present review, the proteins involved in integrin signal transduction and integrin signal transduction pathways were discussed, mainly on the basic mechanisms of integrin signaling and the roles of integrins in bone signal transduction, which may give insight into new therapeutic agents to all kinds of skeletal diseases and new strategies for bone tissue engineering.

关键词： integrin, signal transduction bone absoption bone formation

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The osteogenetic rate in alveolar bone remodeling induced by distraction osteogenesis of the periodontal ligament

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Journal of Medical Colleges of PLA(China) 2006年第3期21卷 182-185页

作者：王爽丰培勋郭雄周洪 Department of Orthodontics and Research Center for Stomatology Stomatological HospitalXi'an Jiaotong UniversityXi'an 710004China Department of Public HealthMedical CollegeXi'an Jiaotong UniversityXi'an 710061China Department of Stomatology Shaanxi Provincial Corps HospitalChinese People's Armed Police ForcesXi'an 710054China Department of Public Health Medical CollegeXi'an Jiaotong UniversityXi'an 710061China Department of Orthodontics and Research Center for Stomatology Stomatological HospitalXi'an Jiaotong UniversityXi'an 710004China

Objective: To observe osteogenetic rate of alveolar bone on the tension side in orthodontic tooth movement through distraction osteogenesis of the periodental ligament quantificationally. Methods: The experiment was carried in 6 dogs. The left side of jaws of each one was set as test or control side, and the other side was control or test side. On the control side, the first premorlar was moved by traditional method on the test side. A self-made distraction device was used on the test side. The newly formed alveolar bone on the tension side of moved tooth was labeled by serial tetracycline fluorochrome. Sections were observed by fluorescence microscope and pictured. Newly formed bone was measured by computer image analysis. Results: The quantity of newly formed bone was significantly different between the two methods. Newly formed bone in rapid tooth movement by distraction osteogenesis of the periodental ligament was more than that in traditional method. Conclusion: The distraction through periodental ligament could induce more rapid bone formation and excite higher osteogenetic activity than traditional method.

关键词： bone formation distraction osteogenesis periodental ligament fluorescence labeling

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The Investigation of Early ApplicationofIcariin Effectonthe bone Metabolism Indexof 5/6 Nephrectomy Mouse

The Investigation of Early ApplicationofIcariin Effectonthe ...

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第十四届中国实验动物科学年会

作者： Zhang Hao Zhang Wei-Qiang Shu Bing Longhua Hospital Shanghai University of Traditional Chinese Medicine (TCM) Spine Research Institute Shanghai University of TCM Key Laboratory of Theory and Therapy of Muscles and bones Ministry of Education (Shanghai University of TCM)

Objective:To investigate the effect of early application of Icariin on the bone Metabolism Index of 5/6 nephrectomy mouse. Method: 40 three month-old Male C57 mouse were selected as research subjects, randomly divided into Icariin group Rocaltrol group Model group and Sham group,10 mouse in each group. For Icariin group and Rocaltrol group the whole left nephron was cut off, 0 ne week later the 2/3 right nephron was cut off. For the Model group the mouse experienced the same surgery but the nephron was remained. For the sham group, no surgery was applied. The mouse in each group was treated with intragastric administration of corresponding drugs, for Icariin group the mouse was treated with Icariin 20 mg/kg/time 5 times/week. For Rocaltrol group the mouse was treated with Rocaltrol 20 mg/kg/time 5 times/week. For Model group and Sham group the mouse was treated with physical saline 20 mg//kg/time 5 times/week. 8 weeks later research materials like serum lumbar4 and nephron were dealt with. Then the nephron function between four groups with SCR,UN,CA and Pwere compared by Elisa. The bone metabolism index like OPG,VD and PTHwere compared between four groups with Elisa. Osterix and MMP9 were tested by immunohistochemical staining. The nephron treated by relative methods were stained with HE, the Lumbar 4 treated by relative methods were stained with HE,Alcian Blueand Masson. Result: Compared with Model group and Sham group the SCR levelofIcariin and Rocaltrol groups were lower(P<0.01),there was no difference between Icariin and Rocaltrol groups;Compared with Model group and Sham group the UN levelofIcariin and Rocaltrol groups were lowe(rP<0.01),there was no difference between Icariin and Rocaltrol groups;Compared with Model group and Sham group the CAlevelofIcariin and Rocaltrol groups were higher(P<0.01),comparedwith Rocaltrol groupthe CA levelofIcariin groupwashigher(P<0.05);Compared with Model group and Sham group the P levelofIcariin and Rocaltrol groups were lowe

关键词： Icariin 5/6 Nephrectomy Mouse Nephron function bone metabolism index bone formation

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