In Vivo Estimation of Osteogenesis by Bone Marrow Cells in a Bi-Phasic Scaffold and in Each of Its Components

作     者：Masataka Yoshikawa Hideyuki Kakigi Ayano Miyamoto Sadaomi Sugimoto Keisuke Nakai Hideaki Ikenaga Takeshi Inamoto Hiroshi Maeda Masataka Yoshikawa;Hideyuki Kakigi;Ayano Miyamoto;Sadaomi Sugimoto;Keisuke Nakai;Hideaki Ikenaga;Takeshi Inamoto;Hiroshi Maeda

作者机构：Department of Endodontics Osaka Dental University Osaka Japan 

出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)

年 卷 期：2016年第9卷第11期

页      面：501-514页

学科分类：080503[工学-材料加工工程] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 080201[工学-机械制造及其自动化] 

主　　题：Bi-Phasic Scaffold Hydroxyapatite Polyvinyl Alcohol Sponge Bone Formation In Vivo 

摘      要：A bi-phasic scaffold consisting of a columnar formaldehyde-acetalized polyvinyl alcohol (PVF) sponge and a cylindrical porous hydroxyapatite (HA) with a hollow center was devised. Rat bone marrow cells (rBMCs) were seeded into the sponge placed in the hollow center of the cylindrical porous HA. The bi-phasic scaffold, a cylindrical porous HA and a PVF sponge separated from a bi-phasic scaffold after rBMC seeding, and a PVF sponge without rBMCs as a negative control, were implanted for 6 weeks into rat dorsal subcutaneous tissue. In each construct, bone formation was examined histologically and osteocalcin was measured immunochemically. Bone formation was observed in the bi-phasic scaffold and also in the cylindrical porous HA isolated from the bi-phasic scaffold. A significant difference in the quantity of osteocalcin was observed between the bi-phasic scaffold and the isolated cylindrical porous HA. No bone formation was found in the isolated PVF sponge. The bi-phasic scaffold as an outer layer of the scaffold seemed to inhibit the outflow of rBMCs from the PVF sponge. This type of bi-phasic scaffold may have two specific characteristics: Attachment of cells both in PVF sponge and cylindrical porous HA.