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A bifunctional MXene-modified scaffold for photothermal therapy and maxillofacial tissue regeneration

Regenerative Biomaterials 2021年第6期8卷 148-158页

作者： Fengji Li Yanling Yan Yanan Wang Yaru Fan Huiru Zou Han Liu Rui Luo Ruixin Li Hao Liu Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction Tianjin Stomatological HospitalThe Affiliated Stomatological Hospital of Nankai UniversityTianjin 300041China Tianjin Medical University Tianjin 300041China Department of Stomatology Shenzhen Luohu Hospital of Traditional Chinese MedicineShenzhen Hospital of Shanghai University of Traditional Chinese MedicineShenzhen 518001China

Oral squamous cell carcinoma is one of the most common malignant tumours in the oral and maxillofacial regions and is highly malignant and prone to recur despite the development of various effective treatments,including surgery and ***,it is difficult to ensure the complete elimination of tumour cells,and maxillofacial bone defects caused by surgery are hard to heal by *** addition,chemoradiotherapy can bring serious ***,it is imperative to develop a postoperative therapy to kill residual squamous cancer cells and repair bone defects without any ***,we prepared a 3D scaffold by a 3D printing technique and freeze-drying method,which contained collagen,silk and hydroxyapatite(CSH)and was functionalized with MXene nanosheets(M-CSH).The considerable photothermal effect with long-term stability can significantly kill squamous CAL-27 cancer cells in vitro and inhibit tumour growth in vivo,increasing the probability of the M-CSH scaffold being applied in the photothermal therapy of oral squamous cell ***,the cell proliferation-and osteogenic-related protein expression of mouse embryonic osteogenic precursors(MC3T3-E1)indicated excellent biocompatibility and osteogenic activity of M-CSH *** good compression modulus(52.8362.25 kPa)and in vivo bone formation performance made it possible to be used as reconstructive materials for bone *** scaffold is likely promising in future tissue engineering,especially for the multifunctional treatment of maxillofacial tumours.

关键词： OSCC MXene 3D printing photothermal therapy bone formation

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Molecular mechanisms of intermuscular bone development in fish:a review

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Zoological Research 2021年第3期42卷 362-376页

作者： Bo Li Yuan-Wei Zhang Xiao Liu Li Ma Jun-Xing Yang Cave Fish Development and Evolution Research Group Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China College of Life Sciences Capital Normal UniversityBeijing 100048China State Key Laboratory of Genetic Resources and Evolution Kunming Institute of ZoologyInnovative Academy of Seed DesignChinese Academy of SciencesKunmingYunnan 650223China Yunnan Key Laboratory of Plateau Fish Breeding Yunnan Engineering Research Center for Plateau-Lake Health and RestorationKunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China

Intermuscular bones(IBs)are slender linear bones embedded in muscle,which ossify from tendons through a process of intramembranous ossification,and only exist in basal *** are essential for fish swimming,but they present a choking risk during human consumption,especially in children,which can lead to commercial risks that have a negative impact on the aquaculture of these *** this review,we discuss the morphogenesis and functions of IBs,including their underlying molecular mechanisms,as well as the advantages and disadvantages of different methods for IB studies and techniques for breeding and generating IB-free fish *** review reveals that the many key genes involved in tendon development,osteoblast differentiation,and bone formation,e.g.,scxa,msxC,sost,twist,bmps,and osterix,also play roles in IB ***,this paper provides useful information for the breeding of new fish strains without IBs via genome editing and artificial selection.

关键词： Intermuscular bones Molecular mechanisms Intramembranous ossification bone formation Genome editing Artificial selection

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Poly（L-lactide）-grafted Bioglass/Poly（lactide-co-glycolide） Scaffolds with Supercritical CO2 Foaming Reprocessing for bone Tissue Engineering

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Chemical Research in Chinese Universities 2017年第3期33卷 499-506页

作者： DONG Shujun WANG Lin LI Qiushi CHEN Xuesi LIU Shujie ZHOU Yanmin VIP Department of Stomatological Hospital of Jilin University Changchun 130021 P. R. China Implatnt Center Stomatologieal Hospital of Jilin University Changchun 130021 P. R. China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changehun 130022 P. R. China

The bioglass particles/poly（lactide-co-glycolide）（BG/PLGA） scaffold has been extensively explored for biomedical applications due to its excellent advantages of mechanical property and controllable degradation rate. In ottr previous studies, the BG nanoparticle sttrface-grafted with poly（L-lactide）（PLLA） could substantially improve the phase compatibility between the polymer matrix and the inorganic phase and the biocompatibility of the scaffolds. However, using the traditional preparation methods to prepare the composite scaffold can barely achieve a high po- rosity and porous connectivity. In this work, the PLLA-grafted bioglass/PLGA（g-BG/PLGA） scaffolds were prepared by supercritical carbon dioxide foaming（Sc-CO2） with before or after particulate leaching（PL） method（Sc-CO2-PL or PL-Sc-CO2 method, PL/Sc-CO2 methods） and their applications in bone replacement and tissue engineering were investigated. The porosities of the g-BG/PLGA scaffolds prepared by the PL/Sc-CO2 methods were higher than 90%, and their mechanical properties had similar values with human cancellous bone. The proliferations of osteoblasts on the scaffolds were dependent on different preparation methods. The PL/Sc-CO2 methods significantly increased the proliferations of the cells. Computed tomography（CT） three-dimensional（3D） reconstruction tomographies of the implantation study for repairing calvarium defects of rabbits demonstrated that the calvarium defects were almost completely filled by the osteotylus in PL/Sc-CO2 method group at 12 week post-surgery, while there was little callus formation in PL method group and untreated control group. These results indicate that the g-BG/PLGA scaffolds prepared by the PL/Sc-CO2 methods exhibit rapid mineralization and osteoconductivity and are the optimal composites for bone repair.

关键词： Grafted bioglass Compisite scaffold Supercritical carbon dioxide foaming bone formation

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Characterization of the essential role of bone morphogenetic protein 9 (BMP9) in osteogenic differentiation of mesenchymal stem cells (MSCs) through RNA interference

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Genes & Diseases 2018年第2期5卷 172-184页

作者： Shujuan Yan Ruyi Zhang Ke Wu Jing Cui Shifeng Huang Xiaojuan Ji Liping An Chengfu Yuan Cheng Gong Linghuan Zhang Wei Liu Yixiao Feng Bo Zhang Zhengyu Dai Yi Shen Xi Wang Wenping Luo Bo Liu Rex C.Haydon Michael J.Lee Russell R.Reid Jennifer Moriatis Wolf Qiong Shi Hue H.Luu Tong-Chuan He Yaguang Weng Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine Chongqing Medical UniversityChongqing 400016China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA The School of Pharmacy and the Affiliated Hospitals of Chongqing Medical University Chongqing 400016China Key Laboratory of Orthopaedic Surgery of Gansu Province and the Department of Orthopaedic Surgery The Second Hospital of Lanzhou UniversityLanzhou730030China Department of Biochemistry and Molecular Biology China Three Gorges University School of MedicineYichang 443002China Department of Surgery The Affiliated Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Orthopaedic Surgery Chongqing Hospital of Traditional Chinese MedicineChongqing 400021China Department of Orthopaedic Surgery Xiangya Second Hospital of Central South UniversityChangsha 410011China Department of Surgery Laboratory of Craniofacial Biology and DevelopmentSection of Plastic SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA

Mesenchymal stem cells(MSCs)are multipotent stem cells and capable of differentiating into multiple cell types including osteoblastic,chondrogenic and adipogenic *** previously identified BMP9 as one of the most potent BMPs that induce osteoblastic differentiation of MSCs although exact molecular mechanism through which BMP9 regulates osteogenic differentiation remains to be fully ***,we seek to develop a recombinant adenovirus system to optimally silence mouse BMP9 and then characterize the important role of BMP9 in osteogenic differentiation of *** two different siRNA bioinformatic prediction programs,we design five siRNAs targeting mouse BMP9(or simB9),which are expressed under the control of the converging H1 and U6 promoters in recombinant adenovirus *** demonstrate that two of the five siRNAs,simB9-4 and simB9-7,exhibit the highest efficiency on silencing exogenous mouse BMP9 in ***,simB9-4 and simB9-7 act synergistically in inhibiting BMP9-induced expression of osteogenic markers,matrix mineralization and ectopic bone formation from ***,our findings demonstrate the important role of BMP9 in osteogenic differentiation of *** characterized simB9 siRNAs may be used as an important tool to investigate the molecular mechanism behind BMP9 osteogenic *** results also indicate that recombinant adenovirus-mediated expression of siRNAs is efficient and sustained,and thus may be used as an effective delivery vehicle of siRNA therapeutics.

关键词： BMP9 bone formation Mesenchymal stem cells Osteogenic differentiation RNA interference Recombinant adenovirus siRNA

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Induced bone by Calcium Ohosphate (Ca-P) Ceramics after 2-years Implantation

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Journal of Wuhan University of Technology(Materials Science) 2009年第5期24卷 781-786页

作者：张志勇 Oral Medicine Department of the Second Hospital Hebei Medical University

The changes of induced bone with four kinds of Ca-P ceramics after 2 years implantation in the dorsal muscles of rabbits were investigated. After 2 years implantation, mature bone （with a lamellar structure after the remodeling process） with bone marrow was observed in HA1100 （HA, micro＋macro porous）, HA900 （HA, micro＋macro porous） and BCP （HA/TCP=7：3, micro＋macro porous）, and no bone formation was observed in HA1200 （HA, macro porous） ceramics. The induced bone neither disappeared nor grew uncontrollably, and was seen inside the pores of the implants or on the outer surface under the fibrous layer after 2 years. No bone formation was observed in the soft tissues distant from the implants. These results indicate that heterotopic bone formation induced by Ca-P materials did not give rise to uncontrolled growth over time, and the induced bone is limited to the inside or around the implants. Moreover, ectopic bone formation on the outer surface of the implants was detected in rabbits after 2 years.

关键词： long-term fate bone formation osteoinduction ceramic

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A bioactive material with dual integrin-targeting ligands regulates specific endogenous cell adhesion and promotes vascularized bone regeneration in adult and fetal bone defects

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Bioactive Materials 2023年第2期20卷 179-193页

作者： Dake Hao Ruiwu Liu Tomas Gonzalez Fernandez Christopher Pivetti Jordan Elizabeth Jackson Edwin Samuel Kulubya Hong-Jiang Jiang Hai-Yang Ju Wen-Liang Liu Alyssa Panitch Kit S.Lam JKent Leach Diana L.Farmer Aijun Wang Department of Surgery School of MedicineUniversity of California DavisSacramentoCA95817United States Institute for Pediatric Regenerative Medicine Shriners Hospitals for ChildrenSacramentoCA95817United States Department of Biochemistry and Molecular Medicine School of MedicineUniversity of California DavisSacramentoCA95817United States Department of Biomedical Engineering University of California DavisDavisCA95616United States Wendeng Orthopaedic Hospital No.1 Fengshan RoadWendeng264400ShandongChina Department of Orthopaedic Surgery School of MedicineUniversity of California DavisSacramentoCA95817United States

Significant progress has been made in designing bone materials capable of directing endogenous cells to promote vascularized bone ***,current strategies lack regulation of the specific endogenous cell populations for vascularized bone regeneration,thus leading to adverse tissue formation and decreased regenerative ***,we engineered a biomaterial to regulate endogenous cell adhesion and promote vascularized bone *** biomaterial works by presenting two synthetic ligands,LLP2A and LXW7,explicitly targeting integrinsα4β1 andαvβ3,respectively,expressed on the surfaces of the cells related to bone formation and vascularization,such as mesenchymal stem cells(MSCs),osteoblasts,endothelial progenitor cells(EPCs),and endothelial cells(ECs).In vitro,the LLP2A/LXW7 modified biomaterial improved the adhesion of MSCs,osteoblasts,EPCs,and ECs via integrinα4β1 andαvβ3,*** an adult rat calvarial bone defect model,the LLP2A/LXW7 modified biomaterial enhanced bone formation and vascularization by synergistically regulating endogenous cells with osteogenic and angiogenic potentials,such as DLX5^(+)cells,osteocalcin^(+)cells,CD34^(+)/CD45-cells and CD31^(+)*** a fetal sheep spinal bone defect model,the LLP2A/LXW7 modified biomaterial augmented bone formation and vascularization without any adverse *** innovative biomaterial offers an off-the-shelf,easy-to-use,and biologically safe product suitable for vascularized bone regeneration in both fetal and adult disease environments.

关键词： Integrin-based ligands Endogenous stem cells bone formation Vascularization Adult and fetal bone defects

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Argonaute(AGO)proteins play an essential role in mediating BMP9-induced osteogenic signaling in mesenchymal stem cells(MSCs)

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Genes & Diseases 2021年第6期8卷 918-930页

作者： Yukun Mao Na Ni Linjuan Huang Jiaming Fan Hao Wang Fang He Qing Liu Deyao Shi Kai Fu Mikhail Pakvasa William Wagstaff Andrew Blake Tucker Connie Chen Russell R.Reid Rex C.Haydon Sherwin H.Ho Michael J.Lee Tong-Chuan He Jian Yang Le Shen Lin Cai Hue H.Luu Departments of Spine Surgery and Musculoskeletal Tumor and NeurosurgeryZhongnan Hospital of Wuhan UniversityWuhanHubei Province430072PR China Molecular Oncology Laboratory Department of Orthopaedic Surgery and Rehabilitation MedicineThe University of Chicago Medical CenterChicagoIL 60637USA Ministry of Education Key Laboratory of Diagnostic Medicine and the School of Laboratory MedicineChongqing Medical UniversityChongqing400016PR China Departments of Nephrology and Obstetrics and GynecologyThe First Affiliated Hospital of Chongqing Medical UniversityChongqing400016PR China Department of Spine Surgery Second Xiangya HospitalCentral South UniversityChangshaHunan Province410011PR China Department of Orthopaedic Surgery Union Hospital of Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei Province430022PR China Section of Plastic Surgery and Laboratory of Craniofacial Biology and Development and Section of Surgical ResearchDepartment of SurgeryThe University of Chicago Medical CenterChicagoIL 60637USA Department of Biomedical Engineering Materials Research InstituteThe Huck Institutes of the Life SciencesThe Pennsylvania State UniversityUniversity ParkPA 16802USA

As multipotent progenitor cells,mesenchymal stem cells(MSCs)can renew themselves and give rise to multiple lineages including osteoblastic,chondrogenic and adipogenic ***’s previously shown that BMP9 is the most potent BMP and induces osteogenic and adipogenic differentiation of ***,the molecular mechanism through which BMP9 regulates MSC differentiation remains poorly *** evidence indicates that noncoding RNAs,especially microRNAs,may play important roles in regulating MSC differentiation and bone *** highly conserved RNA binding proteins,Argonaute(AGO)proteins are essential components of the multi-protein RNA-induced silencing complexes(RISCs),which are critical for small RNA ***,we investigate possible roles of AGO proteins in BMP9-induced lineage-specific differentiation of *** first found that BMP9 upregulated the expression of Ago1,Ago2 and Ago3 in *** engineering multiplex siRNA vectors that express multiple siRNAs targeting individual Ago genes or all four Ago genes,we found that silencing individual Ago expression led to a decrease in BMP9-induced early osteogenic marker alkaline phosphatase(ALP)activity in ***,we demonstrated that simultaneously silencing all four Ago genes significantly diminished BMP9-induced osteogenic and adipogenic differentiation of MSCs and matrix mineralization,and ectopic bone ***,our findings strongly indicate that AGO proteins and associated small RNA biogenesis pathway play an essential role in mediating BMP9-induced osteogenic differentiation of MSCs.

关键词： Argonaute(AGO)proteins BMP9 bone formation Lineage-specific differentiation Mesenchymal stem cells miRNA biogenesis Osteogenic signaling

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Aqueous extract of freeze-dried Protaetia brevitarsis larvae promotes osteogenesis by activating β-catenin signaling

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Asian Pacific Journal of Tropical Biomedicine 2022年第3期12卷 115-123页

作者： Jayasingha Arachchige Chathuranga Chanaka Jayasingha Kyoung Tae Lee Yung Hyun Choi Chang-Hee Kang Mi-Hwa Lee Gi-Young Kim Department of Marine Life Science Jeju National UniversityJeju 63243Republic of Korea Research Institute for Basic Sciences Jeju National UniversityJeju 63243Republic of Korea Forest Biomaterials Research Center National Institute of Forest ScienceJinju 52817Republic of Korea Department of Biochemistry College of Korean MedicineDong-Eui UniversityBusan 47227Republic of Korea Nakdonggang National Institute of Biological Resources Sangju 37242Republic of Korea

Objective:To investigate the effect of an aqueous extract of Protaetia brevitarsis(AEPB)on osteogenesis using preosteoblast MC3T3-E1 cells and zebrafish ***:Flow cytometric analysis was used to measure the *** phosphatase activity was detetmined using p-nitrophenyl phosphate as a *** deposition was detected using alizarin red staining along with osteogenic marker expression in preosteoblast MC3T3E1 *** addition,vertebral formation in zebrafish larvae was detected using calcein staining and osteogenic gene ***:AEPB highly promoted the expression of osteogenic markers including runt-related transcription factor 2,osterix,and alkaline phosphatase,along with elevated levels of mineralization in MC3T3-E1 ***,AEPB accelerated vertebral formation in zebrafish larvae accompanied by upregulated expression of osteogenic ***535,an inhibitor of Wnt/β-catenin,suppressed AEPB-induced osteogenic gene expression and vertebral formation,indicating that AEPB stimulates osteogenesis by activating the Wnt/β-catenin signaling ***:AEPB stimulates osteoblast differentiation and bone formation by activatingβ-***,AEPB is a promising material that induces osteogenesis,and is useful for the treatment of bone resorption diseases.

关键词： Protaetia brevitarsis Osteoblast differentiation bone formation β-catenin MC3T3-E1

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Study on vertical mandibular distraction osteogenesis using magnesium alloy on canine

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Progress in Natural Science:Materials International 2014年第5期24卷 446-451页

作者： Chengyue Wang Shufeng Wang Yusheng Yao Fuzhai Cui Department of Stomatology Centeral Hospital of Jinzhou Clinical Hospital of Liaoning Medical College Stomatological Hospital of Xuzhou Stomatological Hospital of Liaoning Medical College Department of Materials Science & Engineering Tsinghua University

The bone formation feasibility by a novel magnesium alloy device was evaluated using a canine vertical mandibular distraction osteogenesis(DO) model. Osteotomies were performed in the area where last 3 star’s teeth of left mandibular were pulled out before 3 months. Both AZ31 magnesium alloy(n ? 6) and 316 L stainless steel(n ? 6) distraction devices were implanted. The distraction osteogenesis was carried out with a latency of 5 days after mandibular osteotomy. Distraction proceeded at a rate of 0.3 mm/8 h for 7 days and followed by 4 weeks of consolidations. The evaluations were conducted by scanning electron microscopy(SEM) and histological examinations. There were osteoblasts and trabecular bones formations manifestly in both groups. There was no significant difference in the bone mineral density between the two groups. The surface of the magnesium alloy was much more cracked and uneven, resulting from the surface pitting corrosion. The crew nails were closely combined with the surrounding bone tissue. AZ31 magnesium alloy exhibited a certain degradation rate in mandibular and did not post a negative effect on the kidney and liver. The observations in magnesium alloys group is consistent with the stainless steel group.

关键词： Magnesium alloy Distraction osteogenesis Degradability bone formation Canine

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Effects of phosvitin phosphopeptide-Ca complex prepared by efficient enzymatic hydrolysis on calcium absorption and bone deposition of mice

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Food Science and Human Wellness 2022年第6期11卷 1631-1640页

作者： Mengdie Zhao Dong Uk Ahn Songming Li Wei Liu Shengwei Yi Xi Huang National Research and Development Center for Egg Processing College of Food Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China Animal Science Department Iowa State UniversityAmes 50011USA Chongqing Institute for Food and Drug Control Chongqing 401121China

Phosvitin(PV)was treated with high-temperature,mild pressure(HTMP),and enzyme combination,and then phosvitin phosphopeptides-calcium(PPP-Ca)complexes were *** low-calcium specific pathogen free-Kunming(SPF-KM)mice were used to determine the effect of PPP-Ca complexes on intestinal calcium absorption and their utilization for bone *** serum calcium content was the highest with the HTMP-Enz-PPP-Ca treatment(2.19 mmol/L),and it significantly down-regulated the abnormal elevation of serum alkaline phosphatase(AKP)caused by calcium *** low-calcium control group had the lowest calcium deposited to the femur(80.41 mg/g)and the lowest femur bone mineral density(BMD)(0.17 g/cm^(3)),while HTMP-Enz-PPP-Ca significantly improved bone calcium content(94.33 mg/g)and BMD(0.29 g/cm^(3)).The micro-computed tomography(MCT)images showed that the femur with the normal control,PV-Ca,and HTMP-Enz-PPP-Ca treatments had a more compact,complete,and thicker trabecular network than the low-calcium and CaCl_(2)*** results indicated that the organic calcium(HTMP-Enz-PPP-Ca)promoted calcium absorption and bone deposition,and the effect of HTMP-Enz-PPP-Ca was better than the inorganic CaCl_(2).

关键词： Phosvitin phosphopeptide(PPP)-Ca complex SPF KM mice Calcium absorption bone formation

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