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Gene-activated matrix harboring a miR20a-expressing plasmid promotes rat cranial bone augmentation

Regenerative Biomaterials 2021年第2期8卷 34-42页

作者： Rena Shido Yoshinori Sumita Masahito Hara Mayumi Iwatake Shun Narahara Mayumi Umebayashi Kei-ichiro Miura Yukinobu Kodama Izumi Asahina Department of Regenerative Oral Surgery Unit of Translational MedicineNagasaki University Graduate School of Biomedical Sciences1-7-1 SakamotoNagasaki 852-8588Japan Basic&Translational Research Center for Hard Tissue Disease Unit of Translational MedicineNagasaki University Graduate School of Biomedical Sciences1-7-1 SakamotoNagasaki 852-8588Japan Laboratory of Craniofacial Tissue Engineering and Stem Cells Faculty of DentistryMcGill University3640 University StreetM43MontrealQuebec H3A 2B2Canada Department of Hospital Pharmacy Nagasaki University Hospital1-7-1 SakamotoNagasaki 852-8501Japan

Gene-activated matrix(GAM)has a potential usefulness in bone engineering as an alternate strategy for the lasting release of osteogenic proteins but efficient methods to generate non-viral GAM remain to be *** this study,we investigated whether an atelocollagen-based GAM containing naked-plasmid(p)DNAs encoding microRNA(miR)20a,which may promote osteogenesis in vivo via multiple pathways associated with the osteogenic differentiation of mesenchymal stem/progenitor cells(MSCs),facilitates rat cranial bone ***,we confirmed the osteoblastic differentiation functions of generated pDNA encoding miR20a(pmiR20a)in vitro,and its transfection regulated the expression of several of target genes,such as Bambi1 and PPARc,in rat bone marrow MSCs and induced the increased expression of ***,when GAMs fabricated by mixing 100 ll of 2%bovine atelocollagen,20mg b-TCP granules and 0.5mg(3.3 lg/ll)AcGFP plasmid-vectors encoding miR20a were transplanted to rat cranial bone surface,the promoted vertical bone augmentation was clearly recognized up to 8 weeks after transplantation,as were upregulation of VEGFs and BMP4 expressions at the early stages of ***,GAM-based miR delivery may provide an alternative non-viral approach by improving transgene efficacy via a small sequence that can regulate the multiple pathways.

关键词： gene-activated matrix in vivo gene transfer bone augmentation atelocollagen mir20a

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3D-printed bioactive ceramic scaffolds with biomimetic micro/nano-HAp surfaces mediated cell fate and promoted bone augmentation of the bone-implant interface in vivo

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Bioactive Materials 2022年第6期7卷 120-132页

作者： Xiao Liu Yali Miao Haifeng Liang Jingjing Diao Lijing Hao Zhifeng Shi Naru Zhao Yingjun Wang National Engineering Research Center for Tissue Restoration and Reconstruction GuangzhouChina School of Materials Science and Engineering South China University of TechnologyGuangzhou510641China Guangdong Province Key Laboratory of Biomedical Engineering South China University of TechnologyGuangzhou510006China Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of TechnologyGuangzhou510006PR China Innovation Center for Tissue Restoration and Reconstruction South China University of TechnologyGuangzhou510006PR China Department of Orthopedics Zhujiang HospitalSouthern Medical UniversityNo.253 Industrial AvenueHaizhuGuangzhou510280People’s Republic of China Medical Devices Research&Testing Center of SCUT Guangzhou510006PR China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) 510005 GuangzhouChina

Calcium phosphate bio-ceramics are osteo-conductive,but it remains a challenge to promote the induction of bone augmentation and capillary *** surface micro/nano-topography of materials can be recognized by cells and then the cell fate are *** regulation methods of carving surface structures on bio-ceramics employ mineral reagents and organic additives,which might introduce impurity phases and affect the biological *** a previous study,a facile and novel method was utilized with ultrapure water as the unique reagent for hydrothermal treatment,and a uniform hydroxyapatite(HAp)surface layer was constructed on composite ceramics(β-TCP/CaSiO_(3))in *** combined with 3D printing technology,biomimetic hierarchical structure scaffolds were fabricated with interconnected porous composite ceramic scaffolds as the architecture and micro/nano-rod hybrid HAp as the surface *** obtained HAp surface layer favoured cell adhesion,alleviated the cytotoxicity of precursor scaffolds,and upregulated the cellular differentiation of mBMSCs and gene expression of HUVECs in *** vivo studies showed that capillary formation,bone augmentation and new bone matrix formation were upregulated after the HAp surface layer was obtained,and the results confirmed that the fabricated biomimetic hierarchical structure scaffold could be an effective candidate for bone regeneration.

关键词： 3D printed porous scaffold Bioactive ceramics HAp surface layer Cell fate mediation bone augmentation

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Efficacy of concentrated growth factors combined with mineralized collagen on quality of life and bone reconstruction of guided bone regeneration

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Regenerative Biomaterials 2020年第3期7卷 313-320页

作者： Yan Dai Xiao-Hui Han Li-Hua Hu Hai-Wei Wu Sheng-Yun Huang Yu-Peng Lu Department of Oral and Maxillofacial Surgery Central Hospital of ZiboShandong UniversityZibo 255036China Department of Oral and Maxillofacial Surgery Department of Prosthodontics Shandong Provincial Hospital Affiliated to Shandong UniversityJinan 250021China Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong UniversityJinan 250061China School of Materials Science and Engineering Shandong UniversityJinan 250061China

To evaluate the clinical efficacy of concentrated growth factors(CGFs)combined with mineralized collagen(MC)in guided bone regeneration(GBR).A retrospective study involving 29 patients treated with GBR technique,which was performed either CGF and MC complexes or MC *** were inserted simultaneously and cone-beam computed tomography was taken immediately,at 3 and 6 months *** were completed by all patients so as to evaluate the main symptoms and daily activities during the first week after *** outcomes of the two groups were statistically *** implants healed *** in both groups suffered from different levels of discomfort for the reason of swelling,pain and chewing impairment on 1-2 ***,swelling of the Trial group was weaker than the Control *** compared with the Control group,pain levels in Trial group were more rapidly reduced and patients took fewer analgesics from Day ***,the reconstitution mean value of the graft was thicker at 3 and 6 months in Trial *** complex with MC were beneficial to relieve the clinical symptoms,promote the peri-implant bone regeneration and shorten the healing time.

关键词： concentrated growth factors mineralized collagen guided bone regeneration bone augmentation osseointegration

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Periodontally accelerated osteogenic orthodontics with platelet-rich fibrin in an adult patient with periodontal disease:A case report and review of literature

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World Journal of Clinical Cases 2021年第6期9卷 1367-1378页

作者： Min Xu Xiao-Yu Sun Jian-Guang Xu Stomatological Hospital and College Anhui Medical UniversityHefei 230032Anhui ProvinceChina Department of Orthodontics Anhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND In the clinical scenario,adult patients with periodontal diseases and dental malformation,characterized by dental crowding in lower anterior teeth with the thin biotype,often require orthodontic *** case report aimed to evaluate the clinical and radiographic outcomes of periodontally accelerated osteogenic orthodontics(PAOO)combined with autologous platelet-rich fibrin(PRF)in an adult patient with class I malocclusion along with dental crowding,a thin periodontal biotype,and buccal plate *** SUMMARY A 32-year-old female complaining of dental crowding and gingival bleeding was referred to the orthodontic *** patient underwent periodontal risk assessment prior to orthodontic *** was diagnosed with a high risk of gingival recession due to dental crowding,root prominence,loss of buccal plates,and a thin gingival tissue *** treatment regimen included PAOO combined with autologous PRF for alveolar augmentation and interproximal enamel reduction for moderate dental ***,PAOO-assisted orthodontic tooth movement in this case showed enhanced periodontium *** outcomes also showed statistically significant improvements(Pbone augmentation and long-term tissue support in adult orthodontic patients with periodontal disease.

关键词： Periodontally accelerated osteogenic orthodontics Orthodontic tooth movement Platelet-rich fibrin bone augmentation Periodontal biotype Case report

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Implant site development using titanium plate and platelet-rich fibrin for congenitally missed maxillary lateral incisors:A case report

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World Journal of Clinical Cases 2022年第6期10卷 2015-2022页

作者： Tian-Shou Zhang Mahmoud Mudalal Si-Cong Ren Yan-Min Zhou Department of Prosthodontics School and Hospital of StomatologyJilin UniversityChangchun 130021Jilin ProvinceChina Department of Dental Implantology School and Hospital of StomatologyJilin UniversityChangchun 130021Jilin ProvinceChina Department of Oral and Maxillofacial Surgery and Periodontology Faculty of DentistryThe Arab American UniversityJenin 240Palestine

BACKGROUND bone deficiency and soft tissue atrophy in the absence of maxillary lateral incisors are among the most challenging problems for implant *** bone grafting is the gold standard for bone augmentation,but not without ***-rich fibrin(PRF),a biodegradable autologous biomaterial,has been widely used for bone and soft tissue ***,titanium plate is an advantageous barrier due to its good space-maintaining ***,there is a lack of literature on implant site development using titanium plate and PRF for congenitally missing maxillary lateral *** SUMMARY The patient was a 19-year-old girl with a congenitally missing tooth(#12).She underwent implant placement and simultaneous autologous bone grafting with titanium plate and *** the follow-up visit 15 d post-procedure,the vascularization of soft tissue was *** was no swelling or pain after the *** months postoperatively,bone regeneration was ***,the definitive restoration was placed,and the patient was satisfied with the esthetic *** Implant site development using titanium plate and PRF for congenitally missing maxillary lateral incisors is a feasible *** this case,the labial bone plate was displaced but remained connected to the base bone,ensuring blood *** titanium plate fixed the labial bone plate and maintained the osteogenic space,while the PRF provided growth factors and leukocytes for bone and soft tissue ***,the procedure reduced the surgical complexity and adverse reactions,displaying outstanding esthetic outcomes.

关键词： Implant placement Platelet-rich fibrin Missing incisor bone augmentation Soft tissue regeneration Case report

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Hard and soft tissue augmentation with occlusive titanium barriers in jaw vertical defects:a novel approach

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Plastic and Aesthetic Research 2022年第1期9卷 640-653页

作者： Fabio Perret Mario Aimetti Mirko Andreasi Bassi Department of Surgical Sciences CIR Dental SchoolSection of PeriodontologyUniversity of TurinTurin 10127Italy

Aim:The aim of this study was to evaluate hard and soft tissue changes following guided bone regeneration(GBR),with occlusive titanium barriers(OTB),in the rehabilitation of partially edentulous atrophic *** bone gain(VBG),horizontal bone width(HBW),and flap thickness(FT)changes,observed between the first and the second surgical stages,were ***:The study included 35 patients(9 men and 26 women;mean age 60±10.53 years)in need of vertical bone augmentation for implant *** implants were placed,44 with a one-stage approach(Group A)and 26 with a two-stage approach(Group B).VBG,HBW,and FT were measured and statistically ***:VBG for implants placed in Group B was significantly higher than those placed in Group A(P=0.006).The increased HBW in Group B was highly significant compared to that exhibited in Group A(P=0.000).A highly significant difference was found in FT before and after the GBR in the two groups considered together,for both the upper and lower jaws(P=0.000 for both).Conclusions:OTBs are reliable devices in GBR,yielding predictable results in terms of bone *** almost all cases(94.3%),a spontaneous increase of the FT,at the second surgical phase,was *** could be due to the titanium surface features which increases spontaneously the thickness of soft tissues over the OTB.

关键词： Guided bone regeneration occlusive titanium barriers titanium foil non-resorbable barriers soft tissue increase bone augmentation

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