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Effect of Cryopreservation on DNA Integrity and Morphological Structure of boar sperm

Animal Husbandry and Feed Science 2014年第6期6卷 325-327页

作者： Yang Jianping Wu Caihong Zhao Xuting Zhou Chunbao Yao Jing Zhang Bin Zheng Xiaofeng College of Veterinary Medicine Yangzhou University Jiangsu Animal Husbandry and Veterinary College Wuxi Council of Agriculture

The paper was to explore the effect of cryopreservation on DNA integrity and morphological structure of boar sperm, and to explore the protective effect of trehalose and lactose on frozen boar sperm. The morphology, ultrastructure and DNA integrity of sperms were observed by phase contract microscope, scanning electron microscope （SEM） and acridine orange （AO） staining, respectively. The results showed that the normal morphological rate and DNA integrity rate of fro- zen sperms were significantly lower than that of fresh sperms （95.5% and 94.7%, respectively）, and the difference between two frozen groups was also significant （P 〈 0.05 ）. The normal morphological rates in trehalose group and lactose group were 74.7% and 67.6%, while DNA integrity rates in trehalose group and lactose group were 66.4% and 63.2%, respectively. The common deformations of frozen sperms under SEM were partial or complete fracture between head and neck, swollen neck_ dama=ed aemsome stnJetn~.

关键词： boar sperm Cryopreservation DNA Morphological structure

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IMPROVEMENT OF THE QUALITY OF boar CRYOPRESERVATION sperm BY SUPPLEMENTED WITH LAMINARIA JAPONICA POLYSACCHARIDE IN DILUENTS

IMPROVEMENT OF THE QUALITY OF BOAR CRYOPRESERVATION SPERM BY...

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The 5th Annual Conference of the Asian Reproductive Biotechnology Society

作者： Ting ZHANG~a Qing-wang LI~(a,b*) a College of Animal Science & Technology,Northwest A & F University,Yangling,ShaanXi province,712100,P.R.China b Department of Biological Engineering,College of Environment & Chemistry Engineering,YanShan University,Qinhuangdao,HeBei province,066004,P.R.China

＜正＞Laminaria Japonica Polysaccharide （LJP） was added into semen freezing diluents with the concentrations of 0.25,0.5,1.0,1.5 and 2.0 mg/ml to study the effects of LJP on boar cryopreservation *** results indicat... 详细信息

关键词： boar sperm Laminaria Japonica Polysaccharide (LJP) cryopreservation

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Bacteriospermia among smallholder artificial insemination boars in the Philippines and potential associated factors

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Asian pacific Journal of Reproduction 2023年第1期12卷 35-41页

作者： Santiago T.Peña Jr. Ma.Delia A.Pagente Bianca Therese P.Ymas Mark Edd B.Janier Department of Veterinary Basic Sciences College of Veterinary MedicineVisayas State UniversityBaybay CityLeyte 6521Philippines

Objective:To determine the prevalence of bacteriospermia,the bacterial load,and the potential factors associated with bacterial contamination in boar semen collected by local smallholder artificial insemination ***:Fifteen individual raw semen samples were collected from locally available artificial insemination boars owned by different smallholder boar operators within the 5th district of Leyte,Philippines and were subjected to standard bacteriological culture and identification,including a survey of potentially associated *** and bacterial count were determined accordingly,while boar characteristics and collection practices were clustered following agglomerative hierarchical clustering ***:One hundred percent contamination with a bacterial count of(2.01±0.38)×10^(3) CFU/mL was *** least 73.33%of the samples were positive for Bacillus spp.,while other identified isolates included Enterobacter spp.,Staphylococcus spp.,***,Pseudomonas spp.,Citrobacter spp.,and Klebsiella ***:Despite the high prevalence of bacteriospermia,the bacterial count is ***,on-farm practices on boar health and management,semen collection,and sanitation as well as the enhancement of basic protocols to control contamination should be conscientiously considered in smallholder artificial insemination operation.

关键词： Bacteriospermia Local artificial insemination boars boar sperm Artificial insemination Semen quality Smallholder pigs

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Fluorometric Viability Assessment of Capacitated and Acrosome-Reacted boar spermatozoa by Flow Cytometry

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Optics and Photonics Journal 2013年第1期3卷 40-44页

作者： Reyna Fierro Humberto Gonzalez-Marquez Rocio Ortiz Jerome Chevrier Bernard Foliguet Departamento de Ciencias de la Salud Universidad Autonoma Metropolitana-IztapalapaMexico CityMexico Laboratoire d’Histologie Embryologie et Microscopie ElectroniqueUniversite LorraineNancyFrance

sperm capacitation involves functional changes, such as the removal or appearance of specific molecules and changes in the plasma membrane;the acrosome reaction (AR) is an exocytotic event induced by calcium influx, enabling the spermatozoa to penetrate the zona pellucida. These processes can be achieved only if the spermatozoa have good viability;indeed, determination of sperm viability is used for the assessment of semen quality. Membrane integrity and mitochondrial activity are important viability parameters of spermatozoa and fluorescent techniques based on membrane permeability to dyes have been developed to determine these parameters. The aim of this work was to determine the viability of boar sperm (fresh, one hour of capacitation induction and 20 min of AR induction) by flow cytometry using propidium iodide (PI) (1.25 μg/mL) and rhodamine 123 (R123) (0.20 μg/mL). Aliquots of 5 × 105 sperm were incubated with each fluorochrome separately and simultaneously for 10 or 20 min, respectively, at 38℃. The proportion of labeled spermatozoa and their fluorescence intensities were measured using a flow cytometer. The fluorescence index (FI) with PI gradually increased during the incubation and we found significant differences between all the groups. With R123, the FI increased in the capacitated sperm but decreased in the acrosome-reacted sperm, with significant differences between the fresh and capacitated spermatozoa. Our results suggest that the increase in the R123 fluorescence intensity in capacitated spermatozoa is due to changes in the mitochondrial membrane activity because the spermatozoa experienced changes in membrane fluidity and flagellar activation during capacitation. The use of fluorochromes and flow cytometry is a good tool for monitoring many markers of sperm function. Although capacitation and AR processes have been well studied, there is still much information to be elucidated with regard to these complex processes.

关键词： boar sperm Flow Cytometry Propidium Iodide Rhodamine 123 Viability

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