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Study on Response Surface Methodology Optimization for Extraction Process of Cyclocarya paliurus Extract and Its blood sugar-lowering Effect

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Agricultural Biotechnology 2020年第5期9卷 127-133页

作者： Yufeng LI Xiayun LIAO Gang WANG Lichun ZHAO College of Pharmacy Guangxi University of Chinese MedicineNanning 530200China Guangxi Zhuang Yao Medicine Center of Engineering and Technology Nanning 530200China

[Objectives]This study was conducted to discuss the mechanism of the blood sugar-lowering effect of Cyclocarya paliurus extract.[Methods]With *** leaves as the raw material,the ethyl acetate-butyl acetate microwave extraction process of *** was *** the yield of *** extract as an evaluation index,single-factor experiments were carried out on such 6 factors as the dry *** leaf powder size,solid-to-liquid ratio,extraction time,microwave frequency,microwave power,and microwave extraction time,and the extraction and purification process was further optimized by response surface *** normal mice and the hyperglycemic mice modeled by alloxan were injected with *** extract to study the blood sugar-lowering effects of different groups of *** extract.[Results]The optimal process combination for the extraction of *** leaves was obtained:dry *** powder size 90-100 mesh,solid-to-liquid ratio 1∶20(g/ml),extraction time 192 min,microwave frequency 2500 MHz,microwave power 490 W,and extraction time 248 *** these conditions,the yield of *** extract reached the maximum,95.10%,reaching 99.83%of the predicted *** blood sugar-lowering test on mice showed that the *** extract had a good effect on lowering blood sugar,indicating that *** extract can improve the free radical scavenging capacity to a certain extent.[Conclusions]This study provides certain reference for the in-depth study of the physiological effects of *** extract and the comprehensive development and utilization of *** extract.

关键词： Cyclocarya paliurus C.paliurus extract Yield blood sugar lowering Radical scavenging

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