Study on Response Surface Methodology Optimization for Extraction Process of Cyclocarya paliurus Extract and Its Blood Sugar-lowering Effect
作者机构：College of PharmacyGuangxi University of Chinese MedicineNanning 530200China Guangxi Zhuang Yao Medicine Center of Engineering and TechnologyNanning 530200China
出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)
年 卷 期：2020年第9卷第5期
页 面：127-133页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学]
基 金：High-level Innovation Team and Outstanding Scholar Project of Guangxi Colleges and Universities(2019-52) "Qihuang Project"High-level Talent Team Cultivation Project of Guangxi University of Chinese Medicine(2018002) 2019 Nanning City Level Scientific Research and Technology Development Planning Project(20193115)
主 题：Cyclocarya paliurus C.paliurus extract Yield Blood sugar lowering Radical scavenging
摘 要：[Objectives]This study was conducted to discuss the mechanism of the blood sugar-lowering effect of Cyclocarya paliurus extract.[Methods]With *** leaves as the raw material,the ethyl acetate-butyl acetate microwave extraction process of *** was *** the yield of *** extract as an evaluation index,single-factor experiments were carried out on such 6 factors as the dry *** leaf powder size,solid-to-liquid ratio,extraction time,microwave frequency,microwave power,and microwave extraction time,and the extraction and purification process was further optimized by response surface *** normal mice and the hyperglycemic mice modeled by alloxan were injected with *** extract to study the blood sugar-lowering effects of different groups of *** extract.[Results]The optimal process combination for the extraction of *** leaves was obtained:dry *** powder size 90-100 mesh,solid-to-liquid ratio 1∶20(g/ml),extraction time 192 min,microwave frequency 2500 MHz,microwave power 490 W,and extraction time 248 *** these conditions,the yield of *** extract reached the maximum,95.10%,reaching 99.83%of the predicted *** blood sugar-lowering test on mice showed that the *** extract had a good effect on lowering blood sugar,indicating that *** extract can improve the free radical scavenging capacity to a certain extent.[Conclusions]This study provides certain reference for the in-depth study of the physiological effects of *** extract and the comprehensive development and utilization of *** extract.
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