检索结果-南通市图书馆

High-throughput experimental methods for investigating biomolecular condensates

Quantitative Biology 2021年第3期9卷 255-266页

作者： Taoyu Chen Qi Lei Minglei Shi Tingting Li Department of Biomedical Informatics School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China MOE Key Laboratory of Bioinformatics Bioinformatics Division and Center for Synthetic&Systems BiologyTNLISTSchool of MedicineTsinghua UniversityBeijing 100084China

Background:The concept of biomolecular condensate was put forward recently to emphasize the ability of certain cellular compartments to concentrate molecules and comprise proteins and nucleic acids with specific biological functions,from ribosome genesis to RNA *** to their unique role in biological processes,it is crucial to investigate their compositions,which is a primary determinant of condensate ***:Since a wide range of macromolecules comprise biomolecular condensates,it is necessary for researchers to investigate them using high-throughput methodologies while low-throughput experiments are not efficient *** high-throughput methods usually purify interacting protein libraries from condensates before being scanned in mass *** is possible to extract organelles as a whole for specific condensates for further analysis,however,most condensates do not have a distinguishable marker or are sensitive to shear force to be extracted as a *** tagging allows a comprehensive view of interacting proteins of target molecule yet only proteins with strong bonds may be pulled *** labeling serves as a complementary method to label more dynamic proteins with weaker interactions,increasing sensitivity while decreasing ***-based fluorescent screening takes another path by scanning images automatically to illustrate the condensing state of biomolecules within membraneless organelles,which is a unique feature unlike the previous mass spectrometry-based ***:This review presents a rough glimpse into high-throughput methodologies for biomolecular condensate investigation to encourage usage of bioinformatic tools by researchers in relevant fields.

关键词： biomolecular condensates high-throughput phase separation interaction

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

PABP-driven secondary condensed phase within RSV inclusion bodies activates viral mRNAs for ribosomal recruitment

引用

Virologica Sinica 2024年第2期39卷 235-250页

作者： Qiang Zhang Hanzhe Ye Cong Liu Haiwu Zhou Mingbin He Xiaodong Liang Yu Zhou Kun Wang Yali Qin Zhifei Li Mingzhou Chen State Key Laboratory of Virology and Modern Virology Research Center College of Life SciencesWuhan UniversityWuhan430072China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan430072China Hubei Jiangxia Laboratory Wuhan430200China

Inclusion bodies(IBs)of respiratory syncytial virus(RSV)are formed by liquid-liquid phase separation(LLPS)and contain internal structures termed“IB-associated granules”(IBAGs),where anti-termination factor M2-1 and viral mRNAs are ***,the mechanism of IBAG formation and the physiological function of IBAGs are ***,we found that the internal structures of RSV IBs are actual M2-1-free viral messenger ribonucleoprotein(mRNP)condensates formed by secondary ***,the RSV nucleoprotein(N)and M2-1 interact with and recruit PABP to IBs,promoting PABP to bind viral mRNAs transcribed in IBs by RNArecognition motif and drive secondary phase ***,PABP-eIF4G1 interaction regulates viral mRNP condensate composition,thereby recruiting specific translation initiation factors(eIF4G1,eIF4E,eIF4A,eIF4B and eIF4H)into the secondary condensed phase to activate viral mRNAs for ribosomal *** study proposes a novel LLPS-regulated translation mechanism during viral infection and a novel antiviral strategy via targeting on secondary condensed phase.

关键词： Respiratory syncytial virus(RSV) Inclusion bodies(IBs) biomolecular condensates Liquid-liquid phase separation(LLPS) Secondary condensed phase mRNA activation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Phase separation in cGAS-STING signaling

引用

Frontiers of Medicine 2023年第5期17卷 855-866页

作者： Quanjin Li Pu Gao CAS Key Laboratory of Infection and Immunity National Laboratory of BiomacromoleculesCAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing100049China

biomolecular condensates formed by phase separation are widespread and play critical roles in many physiological and pathological ***-STING signaling functions to detect aberrant DNA signals to initiate anti-infection defense and antitumor *** the same time,cGAS-STING signaling must be carefully regulated to maintain immune ***,exciting recent studies have reported that biomolecular phase separation exists and plays important roles in different steps of cGAS-STING signaling,including cGAS condensates,STING condensates,and IRF3 *** addition,several intracellular and extracellular factors have been proposed to modulate the condensates in cGAS-STING *** studies reveal novel activation and regulation mechanisms of cGAS-STING signaling and provide new opportunities for drug ***,we summarize recent advances in the phase separation of cGAS-STING signaling and the development of potential drugs targeting these innate immune condensates.

关键词： biomolecular condensates phase separation cGAS-STING pathway cGAS STING cGAMP interferon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Plant RNA-binding proteins:Phase separation dynamics and functional mechanisms underlying plant development and stress responses

引用

Molecular Plant 2024年第4期17卷 531-551页

作者： Sheng Fan Yu Zhang Shaobo Zhu Lisha Shen Temasek Life Sciences Laboratory 1 Research LinkNational University of Singapore1 Research LinkSingapore 117604Singapore Department of Biological Sciences National University of Singapore14 Science Drive 4Singapore 117543Singapore

RNA-binding proteins(RBPs)accompany RNA from synthesis to decay,mediating every aspect of RNA metabolism and impacting diverse cellular and developmental processes in *** RBPs undergo phase separation along with their bound RNA to form and function in dynamic membraneless biomolecular condensates for spatiotemporal coordination or regulation of RNA *** evidence suggests that phase-separating RBPs with RNA-binding domains and intrinsically disordered regions play important roles in plant development and stress ***,we summarize the current knowledge about how dynamic partitioning of RBPs into condensates controls plant development and enables sensing of experimental changes to confer growth plasticity under stress conditions,with a focus on the dynamics and functional mechanisms of RBP-rich nuclear condensates and cytoplasmic granules in mediating RNA *** also discuss roles of multiple factors,such as environmental signals,protein modifications,and N6-methyladenosine RNA methylation,in modulating the phase separation behaviors of RBPs,and highlight the prospects and challenges for future research on phase-separating RBPs in crops.

关键词： RNA-binding proteins phase separation biomolecular condensates RNA metabolism development stress resilience

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Liquid–liquid phase separation in plants:Advances and perspectives from model species to crops

引用

Plant Communications 2024年第1期5卷 24-40页

作者： Qianwen Liu Wenxuan Liu Yiding Niu Tao Wang Jiangli Dong College of Biological Sciences China Agricultural UniversityBeijing 100193China College of Life Sciences Henan Agricultural UniversityZhengzhou 450002China Key Laboratory of Forage and Endemic Crop Biology Ministry of EducationSchool of Life SciencesInner Mongolia UniversityHohhot 010021China

Membraneless biomolecular condensates play important roles in both normal biological activities and re-sponses to environmental stimuli in living ***‒liquid phase separation(LLPS)is an organi-zational mechanism that has emerged in recent years to explain the formation of biomolecular *** the past decade,advances in LLPS research have contributed to breakthroughs in *** contrast,although LLPS research in plants has progressed over the past 5 years,it has been concentrated on the model plant Arabidopsis,which has limited relevance to agricultural *** this review,we provide an overview of recently reported advances in LLPS in plants,with a particular focus on photomorphogenesis,flowering,and abiotic and biotic stress *** propose that many potential LLPS proteins also exist in crops and may affect crop growth,development,and stress *** possibility presents a great challenge as well as an opportunity for rigorous scientific research on the biological functions and applications of LLPS in crops.

关键词： biomolecular condensates crops intrinsic disordered proteins LLPS potential prediction

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mechanisms and regulation underlying membraneless organelle plasticity control

引用

Journal of Molecular Cell Biology 2021年第4期13卷 239-258页

作者： Hazrat Ismail Xu Liu Fengrui Yang Junying Li Ayesha Zahid Zhen Dou Xing Liu Xuebiao Yao MOE Key Laboratory for Membraneless Organelles&Cellular Dynamics and CAS Center for Excellence in Molecular Cell Science Hefei National Laboratory for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230027China Keck Center for Organoids Plasticity Control AtlantaGA 30310USA Anhui Key Laboratory for Cellular Dynamics&Chemical Biology Hefei 230027China

Evolution has enabled living cells to adopt their structural and functional complexity by organizing intricate cellular compartments,such as membrane-bound and membraneless organelles(MLOs),for spatiotemporal catalysis of physiochemical reactions essential for cell plasticity *** evidence and view support the notion that MLOs are built by multivalent interactions of biomolecules via phase separation and transition *** healthy cells,dynamic chemical modifications regulate MLO plasticity,and reversible phase separation is essential for cell *** evidence revealed that aberrant phase separation results in numerous neurodegenerative disorders,cancer,and other *** this review,we provide molecular underpinnings on(i)mechanistic understanding of phase separation,(ii)unifying structural and mechanistic principles that underlie this phenomenon,(iii)various mechanisms that are used by cells for the regulation of phase separation,and(iv)emerging therapeutic and other applications.

关键词： liquid-liquid phase separation membraneless organelles biomolecular condensates intrinsically disordered proteins post-translational modifications

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Visualizing carboxyl-terminal domain of RNA polymerase Ⅱ recruitment by FET fusion protein condensates with DNA curtains

引用

Biophysics Reports 2022年第2期8卷 80-89页

作者： Linyu Zuo Jiawei Ding Zhi Qi Center for Quantitative Biology and Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdiscip-linary Studies Peking University

Many recent references show that living cells can form some membrane-less organelles by liquid–liquid phase separation(LLPS) of biomolecules, like proteins and nucleic acids. LLPS has been confirmed to link with many important biological functions in living cells, and one of the most important functions of biomolecular condensates is in the field of RNA transcription. Many studies confirm that mammalian RNA polymerase Ⅱ(Pol Ⅱ) molecules containing the CTD with different phosphorylation level are purposed to shuttle between initiation condensates and elongation condensates of RNA transcription. Traditional ensemble assays often experience difficulties in quantitively and directly recording the transient recruitment of Pol Ⅱ CTD. Novel single-molecule approach — DNA curtains can be used to directly visualize biomolecular condensates formation and also recruitment of RNA polymerase Ⅱ(Pol Ⅱ) carboxyl-terminal domain(CTD) at the target sites in solution and in real time. This method can offer the potential for new insights into the mechanism of gene transcription. Here, we highlight the detailed protocol of DNA curtains method for studying LLPS.

关键词： Liquid–liquid phase separation (LLPS) biomolecular condensates Single-molecule biophysics DNA curtains In vitro transcription assay

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Liquid-liquid phase separation as a major mechanism of plant abiotic stress sensing and responses

引用

Stress Biology 2023年第1期3卷 610-620页

作者： Xin Liu Jian-Kang Zhu Chunzhao Zhao Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghaiChina Institute of Advanced Biotechnology and School of Life Sciences Southern University of Science and TechnologyShenzhenChina Center for Advanced Bioindustry Technologies Chinese Academy of Agricultural SciencesBeijingChina

Identification of environmental stress sensors is one of the most important research topics in plant abiotic stress *** strategies to identify stress sensors or early signaling components based on the cell membrane as a primary site of sensing and calcium signal as a second messenger have had only limited ***,the current theoretical framework underlying stress sensing in plants should be reconsidered and additional mechanisms need to be ***,accumulating evidence has emerged to suggest that liquid-liquid phase separation(LLPS)is a major mechanism for environmental stress sensing and response in *** this review,we briefly introduce LLPS regarding its concept,compositions,and dynamics,and then summarize recent progress of LLPS research in plants,emphasizing the contribution of LLPS to the sensing of various environmental stresses,such as dehydration,osmotic stress,and low and high ***,we propose strategies to identify key proteins that sense and respond to environmental stimuli on the basis of LLPS,and discuss the research directions of LLPS in plant abiotic stress responses and its potential application in enhancing stress tolerance in crops.

关键词： Liquid-liquid phase separation biomolecular condensates Stress sensors Osmotic stress High temperature

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tuning the rheostat of immune gene translation

引用

Stress Biology 2023年第1期3卷 95-97页

作者： Shuai Huang Department of Molecular Genetics The Ohio State UniversityColumbusOH 43210USA

biomolecular condensates assembled through phase transitions regulate diverse aspects of plant growth,develop-ment,and stress *** biomolecular condensates control plant immunity is poorly *** Nature Plants,a new study(Zhou et al.,Nat Plants 9:289-301,2023)reveals how plants assemble translational condensates to balance tissue health and disease resistance.

关键词： Plant immunity Liquid-liquid phase separation biomolecular condensates Gene translation Cell death Arabidopsis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A simple FCS method to quantify liquid-liquid phase separation beyond the optical diffraction limit

A simple FCS method to quantify liquid-liquid phase separati...

引用

第十一届全国化学生物学学术会议

作者： Sijia Peng Weiping Li Wenjing Xing Yirong Yao Pilong Li 陈春来 School of Life Sciences Tsinghua-Peking Joint Center for Life SciencesBeijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureTsinghua University 清华大学

Liquid-liquid phase separation,driven by multivalent macromolecular interactions,causes formation of membraneless compartments,which are biomolecular condensates containing concentrated *** condensates are involved in diverse cellular *** by current detection methods,free-diffusing condensates and condensates smaller than micron-scale are less well *** is also unclear what is the lower limit of the sizes of functional phase-separated ***,we present a fluorescence correlation spectroscopy method to quantify sizes of biomolecular condensates beyond the optical diffraction limit and binding affinities of recruited molecules in *** capture small-sized condensates formed at concentrations which are up to 10 folds lower than the detection limit of confocal fluorescence microscopy,whose radiuses are 20-70 *** our phase separation system,we quantify that a client molecule is concentrated by about 30 folds in a sizeindependent *** results suggest that,in addition to micron-scale condensates,small-sized condensates are likely to play important roles in cells as well.

关键词： fluorescence correlation spectroscopy liquid-liquid phase separation biomolecular condensates

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：