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CCS Chemistry 2022年第3期4卷 1044-1053页

作者： Zhaohong Wang Liping Chen Dandan Wang Zhenyao Ding Xiqi Zhang Xinjian Feng Lei Jiang College of Chemistry Chemical Engineering and Materials ScienceSoochow UniversitySuzhou 215123 Key Laboratory of Bio-Inspired Materials and Interfacial Science Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190 School of Future Technology University of Chinese Academy of SciencesBeijing 101407

Developing photoelectrochemical(PEC)bioassays based on the principle of a photocathodic measurement of enzymatic product H_(2)O_(2) is highly attractive because it can naturally avoid interfering signals arising from reductive species inherent to biofluids.However,fluctuant oxygen levels in the analyte solution can compromise the accuracy of photocathodic Bioanalysis and restrict its application because oxygen reduction potential is similar to H_(2)O_(2).Herein,we addressed this restriction by constructing a triphase biophotocathode with air–liquid–solid joint interfaces by immobilizing an oxidase enzyme film on the tip part of superhydrophobic p-type semiconductor nanowire arrays.Such a triphase biophotocathode has a reaction zone with steady and air phasedependent oxygen concentration which stabilizes and increases the oxidase kinetics,and enables the photocathodic measurement principle in reliable PEC bioassay development with high selectivity,good accuracy,and a wide linear detection range.Moreover,the biophotocathode shows good stability during repeated testing under light illumination.This reliable PEC bioassay system has broad potential in the fields of disease diagnosis,medical research,and environmental monitoring.

关键词： superhydrophobicity triphase interface photocathode photoelectrochemical Bioanalysis

DNA Technology-assisted Signal Amplification Strategies in Electrochemiluminescence Bioanalysis

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Journal of Analysis and Testing 2021年第2期5卷 95-111页

作者： Yue Cao Cheng Ma Jun-Jie Zhu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Sensitive and accurate detection of biological analytes,such as proteins,genes,small molecules,ions,cells,etc.,has been a significant project in life science.Signal amplification is one of the most effective approaches to improve the sensitivity of Bioanalysis.Taking advantage of specific base pairing,programmable operation,and predictable assembly,DNA is flexible and suitable to perform the signal amplification procedure.In recent years,signal amplification strategies by means of DNA technology have been widely integrated into the construction of electrochemiluminescence(ECL)biosensors,achieving desirable analytical performance in clinical diagnosis,biomedical research,and drug development.To the best of our knowledge,these DNA signal amplification technologies mainly include classical polymerase chain reaction,and various amplification approaches conducted under mild conditions,such as rolling circle amplification(RCA)or hyperbranched RCA,cleaving enzyme-assisted amplification,DNAzyme-involved amplification,toehold-mediated DNA strand displacement amplification without enzyme participation,and so on.This review overviews the recent advancements of DNA signal amplification strategies for Bioanalysis in the ECL realm,sketching the creative trajectory from strategies design to ultrasensitive ECL platform construction and resulting applications.

关键词： Electrochemiluminescence DNA technology Signal amplification strategies Bioanalysis

Bioanalysis in single cells: current advances and challenges

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Science China Chemistry 2020年第5期63卷 564-588页

作者： Hong-Yuan Chen State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Cells, basic units of living structures and functions, build up a complicated small world, and their order and complexity resemble a small universe. The detailed understanding and elucidation of the matter transport and energy conversion mechanisms within a single cell will expand our knowledge about the origin and evolution of life, the disease mechanisms and much more. In past decades, single-cell analysis has been rapidly and significantly improved and various methodologies have been developed to reveal the complexity of mass, energy, and information within single cells. In this review, we focused on the methods developed in recent years for single-cell analysis, including electrochemical method, optical method, and mass spectrometry method. We reviewed the recent advances and representative studies in this research field, and also discussed the strengths and limitations of each method. Finally, we presented the existing technical challenges and further directions for single-cell analysis.

关键词： Bioanalysis single-cell electrochemistry single-cell optical analysis single-cell mass spectrometry

Bioanalysis and Pharmacokinetics of Eight Active Components from Huanglian Jiedu Decoction in Rat Plasma by LC-ESI-MS/MS Method

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Chinese Herbal Medicines 2014年第3期6卷 198-210页

作者： Cheng-cheng Peng Shu-ping Wang Hui-zi Jin Jian-fei Tao Guo-wei Wang Pan-lei Wei Xu-feng Zhang Le Li Run-hui Liu Wei-dong Zhang School of Pharmacy Shanghai Jiao Tong University School of Pharmacy Second Military Medical University Pharmacy Department Shanghai Yangsi Hosipital School of Chemistry and Environmental Engineering Shanghai Institute of Technology

Objective To develop a sensitive and rapid liquid chromatography-electrospray ionization-tandem mass spectrometry （LC-ESI-MS/MS） method for the simultaneous determination of wogonin, coptisine, berberine, palmatine, jatrorrhizine, phellodendrine, magnoflorine, and wogonoside in rat plasma and to evaluate the pharmacokinetic characteristics of Huanglian Jiedu Decoction （HJD）. Methods LC separation was performed on an Acquity HSS T3 column （100 mm × 2.1 mm, 1.8 μm） using gradient elution with the mobile phase consisting of acetonitrile and 0.1% formic acid water. The detection was accomplished by using positive electrospray ionization in multiple-reaction monitoring mode. Plasma samples were pretreated by protein precipitation. Results The method showed a good linearity over a wide concentration range （r^2 〉 0.99）. The lower limits of quantification were 0.20 ng/mL for coptisine and phellodendrine, 0.48 ng/mL for berberine, 0.10 ng/mL for jatrorrhizine, 0.32 ng/mL for magnoflorine, 0.30 ng/mL for palmatine, and 4.80 ng/mL for wogonin and wogonoside, respectively. The intra- and inter-day precision of the analytes was less than 12.11%, while the accuracy was between -14.46% and 4.86%. The mean recovery of all the analytes ranged from 93.10% to 110.91%. Conclusion This validated method offers thee advantages of high sensitivity. It is successfully applied to evaluating the pharmacokinetic properties of HJD.

关键词： alkaloid Bioanalysis flavonoid Huanglian Jiedu Decoction LC-ESI-MS/MS pharmacokinetics

Phage Display Based Bioanalysis and Biosensing

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Phage Display Based Bioanalysis and Biosensing

Attenuated Total Reflection Surface-Enhanced Infrared Absorption Spectroscopy: a Powerful Technique for Bioanalysis

中国化学会第30届学术年会

作者： Aihua Liu Lei Han Huan Qi Pei Liu Fei Wang Qiaolin Lang Institute for Biosensing and College of Chemical Science and EngineeringQingdao University

Specific probes against targets can be selected from the landscape phage library f8/8 displaying random octapeptides on the pⅧ coat protein of the phage fd-tet,and demonstrating many excellent features including multivalency,stability,and high structural homogeneity.In this talk,the advanced phage clones binding with targets were screened from the landscape phage library will be addressed.Furthermore,a wide range of applications developed in our laboratory such as phage ELISA for biomarker detection,peptide microarray,nanomedicine,label-free electrochemical impedance cytosensor will be presented.Taken together,the phage display technology should become efficient and cost-effective in Bioanalysis or biosensing.

关键词： Phage display Specific probes Bioanalysis Phage ELISA Cytosensor

来源： cnki会议评论

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Journal of Analysis and Testing 2017年第1期1卷 71-83页

作者： Jian Li Bo Zheng Qian-Wen Zhang Yang Liu Cai-Feng Shi Feng-Bin Wang Kang Wang Xing-Hua Xia State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210093China

Attenuated total reflection surface-enhanced infrared absorption spectroscopy(ATR-SEIRAS)has recently been proven to be a powerful tool for Bioanalysis.It enables in situ and in real-time observation of dynamic processes occurring on specific interface,revealing rich structural and functional information of biomolecules at sub monolayer level.The aim of this general review was to give an overview of the cutting edge applications of ATRSEIRAS.We start with description of the basic configuration of the standard ATR-SEIRAS platform.The enhanced mechanisms and methods to fabricate enhanced substrates are then presented.We discuss the recent developments,challenges and applications of ATR-SEIRAS in Bioanalysis,mainly focusing on DNA analysis,protein behavior and cell properties.Finally,further development of the ATRSEIRAS technique with enhanced sensitivity,improved time and spatial resolutions will be prospected.

关键词： Surface-enhanced infrared absorption spectroscopy ATR-SEIRAS Bioanalysis DNA Protein Cell

Recent progress in aptamer-based microfluidics for the detection of circulating tumor cells and extracellular vesicles

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Journal of Pharmaceutical Analysis 2023年第4期13卷 340-354页

作者： Duanping Sun Ying Ma Maoqiang Wu Zuanguang Chen Luyong Zhang Jing Lu Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances Center for Drug Research and DevelopmentGuangdong Pharmaceutical UniversityGuangzhou510006China Key Specialty of Clinical Pharmacy The First Affiliated Hospital of Guangdong Pharmaceutical UniversityGuangzhou510699China National and Local United Engineering Lab of Druggability and New Drugs Evaluation School of Pharmaceutical SciencesSun Yat-Sen UniversityGuangzhou510006China

Liquid biopsy is a technology that exhibits potential to detect cancer early,monitor therapies,and predict cancer prognosis due to its unique characteristics,including noninvasive sampling and real-time analysis.Circulating tumor cells(CTCs)and extracellular vesicles(EVs)are two important components of circulating targets,carrying substantial disease-related molecular information and playing a key role in liquid biopsy.Aptamers are single-stranded oligonucleotides with superior affinity and specificity,and they can bind to targets by folding into unique tertiary structures.Aptamer-based microfluidic platforms offer new ways to enhance the purity and capture efficiency of CTCs and EVs by combining the advantages of microfluidic chips as isolation platforms and aptamers as recognition tools.In this review,we first briefly introduce some new strategies for aptamer discovery based on traditional and aptamer-based microfluidic approaches.Then,we subsequently summarize the progress of aptamer-based microfluidics for CTC and EV detection.Finally,we offer an outlook on the future directional challenges of aptamer-based microfluidics for circulating targets in clinical applications.

关键词： Aptamer Microfluidic Circulating tumor cells Extracellular vesicles Bioanalysis

Current developments of bioanalytical sample preparation techniques in pharmaceuticals

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Journal of Pharmaceutical Analysis 2022年第4期12卷 517-529页

作者： Rahul G.Ingle Su Zeng Huidi Jiang Wei-Jie Fang Institute of Drug Metabolism and Pharmaceutical Analysis College of Pharmaceutical SciencesZhejiang UniversityHangzhou310058China Hangzhou Institute of Innovative Medicine Zhejiang UniversityHangzhou310016China

Sample preparation is considered as the bottleneck step in Bioanalysis because each biological matrix has its own unique challenges and complexity.Competent sample preparation to extract the desired analytes and remove redundant components is a crucial step in each bioanalytical approach.The matrix effect is a key hurdle in bioanalytical sample preparation,which has gained extensive consideration.Novel sample preparation techniques have advantages over classical techniques in terms of accuracy,automation,ease of sample preparation,storage,and shipment and have become increasingly popular over the past decade.Our objective is to provide a broad outline of current developments in various bioanalytical sample preparation techniques in chromatographic and spectroscopic examinations.In addition,how these techniques have gained considerable attention over the past decade in bioanalytical research is mentioned with preferred examples.Modern trends in bioanalytical sample preparation techniques,including sorbent-based microextraction techniques,are primarily emphasized.

关键词： Bioanalysis Biological matrices Matrix effect Microextraction Sample preparationtechnique

Metabolomics Combined with Network Pharmacology Uncovers Effective Targets of Tao-Hong-Si-Wu Decoction for Its Protection from Sepsis-Associated Acute Lung Injury

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Journal of Analysis and Testing 2023年第2期7卷 172-186页

作者： Jinzhi He Longfei Wang Yang Wang Zeng Li Feihu Chen Zhicheng Liu Anhui Provincial Laboratory of Infl ammatory and Immunity Disease Anhui Institute of Innovative Drugs School of PharmacyAnhui Medical UniversityHefei 230032China Shanxi Key Laboratory of Big Data for Clinical Decision Research School of ManagementShanxi Medical UniversityTaiyuan 030000China

Sepsis-induced acute lung injury(ALI)is a leading cause of death among septic complications.Tao-Hong-Si-Wu decoction(TSD),a classical recipe from traditional Chinese medicine used for treating ischemic stroke,has been recently reported to alleviate inflammation and inflammation-stimulated injuries related to the pathology of ALI.Here,we first observed the therapeutic effect of TSD on sepsis-induced ALI.Based on integrated metabolomics and network pharmacology analysis(NPA)techniques,we aim to understand the mechanism of TSD alleviating ALI.TSD’s effects were observed in rats modeled by cecal ligation and puncture(CLP)and rat macrophages stimulated by lipopolysaccharide(LPS).Metabolomics analyses were applied to determine the ingredients in the medicine and key metabolites correlated to the NPA for the prediction of TSD targets.Gene and protein expressions of the key predicted targets were evaluated in the lung tissue and macrophages of septic model rat by quantitative polymerase chain reaction(PCR)and enzyme-linked immunosorbent assays,respectively.TSD improved survival rate and protected against lung injury in CLP rats.Eleven endogenous metabolites were related to TSD’s actions.TSD significantly suppressed IL-6 and TNF-αsecretions and their gene expressions both in the lung tissue of the model rats and in LPS-stimulated macrophages.TSD also restored decreased lung protein expression of VEGFA in septic model rats.Targeted proteins and their affecting metabolites were finally validated in an external test set of rats.This study shows that metabolomics coupled with NPA is a promising approach to explore potential targets of medicine with complex compositions.

关键词： Metabolomics Drug eff ect analysis Sepsis Traditional Chinese medicine Cytokines Bioanalysis

Lipid–oligonucleotide conjugates for bioapplications

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National Science Review 2020年第12期7卷 1933-1953页

作者： Xiaowei Li Kejun Feng Long Li Lu Yang Xiaoshu Pan Hoda Safari Yazd Cheng Cui Juan Li Leonid Moroz Yujia Sun Bang Wang Xiang Li Tong Huang Weihong Tan Center for Research at Bio/Nano Interface Department of Chemistry and Department of Physiology and Functional GenomicsHealth Cancer CenterUF Genetics Institute and Mc Knight Brain InstituteUniversity of Florida School of Chemistry and Materials Engineering Huizhou University The Cancer Hospital of the University of Chinese Academy of Sciences Institute of Cancer and Basic Medicine(IBMC) Chinese Academy of Sciences Molecular Science and Biomedicine Laboratory (MBL) State Key Laboratory for Chemo/BioSensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of Biologyand Aptamer Engineering Center of Hunan ProvinceHunan University

Lipid–oligonucleotide conjugates(LONs) are powerful molecular-engineering materials for various applications ranging from biosensors to biomedicine.Their unique amphiphilic structures enable the self-assembly and the conveyance of information with high fidelity.In particular,LONs present remarkable potential in measuring cellular mechanical forces and monitoring cell behaviors.LONs are also essential sensing tools for intracellular imaging and have been employed in developing cell-surface-anchored DNA nanostructures for biomimetic-engineering studies.When incorporating therapeutic oligonucleotides or small-molecule drugs,LONs hold promise for targeted therapy.Moreover,LONs mediate the controllable assembly and fusion of vesicles based on DNA-strand displacements,contributing to nanoreactor construction and macromolecule delivery.In this review,we will summarize the general synthesis strategies of LONs,provide some characterization analysis and emphasize recent advances in bioanalytical and biomedical applications.We will also consider the relevant challenges and suggest future directions for building better functional LONs in nanotechnology and materials-science applications.

关键词： lipid–oligonucleotide DNA nanostructure biosensor biomedicine Bioanalysis nanoreactor