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basic fibroblast growth factor and fibroblast growth factor Receptor-1 in Human Meningiomas

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2005年第1期25卷 75-77页

作者：易伟陈坚 Filimon H. Golwa 薛德麟 Department of Neurosurgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

The expression of basic fibroblast growth factor (bFGF) and fibroblast growth factor receptor-1 (FGFR-1) in human meningiomas and the relationships between their expression and the tumors' histological features and angiogenesis were investigated by means of immunohistochemical technique. The expression of bFGF and FGFR-1 was detected by antibody of bFGF or FGFR-1. The tumors' angiogenesis was evaluated by microvascular density (MVD) and, which was observed by use of CD34-antibody immunohistochemically. The results showed that there were varied degrees of the expression of bFGF and FGFR-1 proteins in meningiomas. The expression was correlated with the tumors' histological characters and angiogenesis. It was concluded that bFGF and FGFR-1 might play important roles in meningiomas' angiogenesis and proliferation. The expression positive rate of bFGF and FGFR-1 may provide an indication of evaluating the histological and malignant degree of the tumor.

关键词： meningiomas basic fibroblast growth factor fibroblast growth factor receptor-1 microvascular density immunohistochemistry

basic fibroblast growth factor attenuates the degeneration of injured spinal cord motor endplates

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Neural Regeneration Research 2013年第24期8卷 2213-2224页

作者： Jianlong Wang Jianfeng Sun Yongxiang Tang Gangwen Guo Xiaozhe Zhou Yanliang Chen Minren Shen Department of Orthopedics Third Xiangya Hospital of Central South University Department of Orthopedics Yichang Central People's Hospital Department of Nuclear Medicine Xiangya Hospital of Central South University

The distal end of the spinal cord and neuromuscular junction may develop secondary degeneration and damage following spinal cord injury because of the loss of neural connections. In this study, a rat model of spinal cord injury, established using a modified Allen＇s method, was injected with basic fibroblast growth factor solution via subarachnoid catheter. After injection, rats with spinal cord injury displayed higher scores on the Basso, Beattie and Bresnahan locomotor scale. Motor function was also well recovered and hematoxylin-eosin staining showed that spinal glial scar hyperplasia was not apparent. Additionally, anterior tibial muscle fibers slowly, but progressively, atrophied. Immu- nohistochemical staining showed that the absorbance values of calcitonin gene related peptide and acetylcholinesterase in anterior tibial muscle and spinal cord were similar, and injection of basic fi- broblast growth factor increased this absorbance. Results showed that after spinal cord injury, the distal motor neurons and motor endplate degenerated. Changes in calcitonin gene related peptide and acetylcholinesterase in the spinal cord anterior horn motor neurons and motor endplate then occurred that were consistent with this regeneration. Our findings indicate that basic fibroblast growth factor can protect the endplate through gene related peptide and acetylcholinesterase cord. attenuating the decreased expression of calcitonin n anterior horn motor neurons of the injured spinal

关键词： neural regeneration spinal cord injury motor endplate basic fibroblast growth factor calcJtonJngene related peptide acetylcholinesterase subarachnoid catheter grants-supported paper neu-roregeneration

basic fibroblast growth factor increases the numbe of endogenous neural stem cells and inhibits the expression of amino methyl isoxazole propionic acid receptors in amyotrophic lateral sclerosis mice

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Neural Regeneration Research 2012年第10期7卷 761-765页

作者： Weihui Huang Dawei Zang Yi Lu Ping Jiang Department of Neurology Tianjin First Center Hospital Tianjin Medical University Tianjin 300192 China

This study aimed to investigate the number of amino methyl isoxazole propionic acid （AMPA） receptors and production of endogenous neural stem cells in the SOD1 G93AG1H transgenic mouse model of amyotrophic lateral sclerosis, at postnatal day 60 following administration of basic fibroblast growth factor （FGF-2）. A radioligand binding assay and immunohistochemistry were used to estimate the number of AMPA receptors and endogenous neural stem cells respectively. Results showed that the number of AMPA receptors and endogenous neural stem cells in the brain stem and sensorimotor cortex were significantly increased, while motor function was significantly decreased at postnatal days 90 and 120. After administration of FGF-2 into mice, numbers of endogenous neural stem cells increased, while expression of AMPA receptors decreased, whilst motor functions were recovered. At postnatal day 120, the number of AMPA receptors was negatively correlated with the number of endogenous neural stem cells in model mice and FGF-2-treated mice. Our experimental findings indicate that FGF-2 can inhibit AMPA receptors and increase the number of endogenous neural stem cells, thus repairing neural injury in amyotrophic lateral sclerosis mice.

关键词： amino methyl isoxazole propionic acid receptor amyotrophic lateral sclerosis basic fibroblast growth factor endogenous neural stem cells

basic fibroblast growth factor gene transfection in repair of internal carotid artery aneurysm wall

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Neural Regeneration Research 2012年第36期7卷 2915-2921页

作者： Lei Jiao Ming Jiang Jinghai Fang Yinsheng Deng Zejun Chen Min Wu Department of Neurosurgery Hospital Affiliated to Jiangsu University

Surgery or interventional therapy has some risks in the treatment of cerebral aneurysm. We established an internal carotid artery aneurysm model by dripping elastase in the crotch of the right internal and external carotid arteries of New Zealand rabbits. Following model induction, lentivirus carrying basic fibroblast growth factor was injected through the ear vein. We found that the longer the action time of the lentivirus, the smaller the aneurysm volume. Moreover, platelet-derived growth factor expression in the aneurysm increased, but smooth muscle 22 alpha and hypertension-related gene 1 mRNA expression decreased. At 1,2, 3, and 4 weeks following model establishment, following 1 week of injection of lentivirus carrying basic fibroblast growth factor, the later the intervention time, the more severe the blood vessel damage, and the bigger the aneurysm volume, the lower the smooth muscle 22 aJpha and hypertension-related gene ~ mRNA expression. Simultaneously, platelet-derived growth factor expression decreased. These data suggest that recombinant lentivirus carrying basic fibroblast growth factor can repair damaged cells in the aneurysmal wall and inhibit aneurysm dynamic growth, and that the effect is dependent on therapeutic duration.

关键词： basic fibroblast growth factor lentivirus aneurysm vascular smooth muscle cells hypertension-related gene 1 smooth muscle 22 alpha platelet-derived growth factor gene therapy brain injury neural regeneration

basic fibroblast growth factor improves learning and memory functions in chronic stress mice

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Neural Regeneration Research 2011年第19期6卷 1473-1477页

作者： Xian Qu Chunying Li Hongchang Liu Chang Su basic Medical College of Beihua University Jilin 132013 Jilin Province China Jilin Provincial Qian Wei Hospital Changchun 130000 Jilin Province China

Four weeks of uncertain stress was used to establish an animal model of chronic stress. basic fibroblast growth factor was injected daily for 15 days following stress induction. Cell morphology in the hippocampal CA3 region of chronic stress mice revealed cell damage. Nitric oxide content and calcium concentration were significantly increased in the hippocampus, and learning and memory functions were significantly decreased. After basic fibroblast growth factor intervention, Ca2~ overload was decreased and neuronal damage was relieved in hippocampal neurons, which improved learning and memory functions in chronic stress mice. Latency was prolonged and the number of errors was decreased in a passive avoidance test.

关键词： basic fibroblast growth factor chronic stress step-down test passive avoidance test learning and memory nitric oxide neural regeneration

Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第12期11卷 895-904页

作者： Shao-ping NIE Xiao WANG Shi-bin QIAO Qiu-tang ZENG Ju-quan JIANG Xiao-qing LIU Xiang-ming ZHU Guo-xiang CAO Chang-sheng MA Department of Cardiology Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Union Hospital of Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Objective： Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited by a relatively short half-life of growth factors. Fibrin glue （FG） provides a reservoir for controlledrelease of growth factors. The aim of this study was to evaluate the effects of basic flbroblast growth factor （bFGF） incorporating FG on angiogenesis and cardiac performance in a canine infarct model. Methods： Acute myocardial infarction was induced by ligation of the left anterior descending coronary artery （LAD）. Group Ⅰ （n=6） underwent ligation of LAD alone. In Group Ⅱ, transmural channels were created in the infarct area （n=6）. In Group Ⅲ, nontransmural channels were created to locate FG cylinders containing bFGF （n=6）. Eight weeks after operation, myocardial perfusion was assessed by single photon emission computed tomography, cardiac function by echocardi- ography, and vascular development by immunohistochemical staining. Results： Total vascular density and the number of large vessels （internal diameter ≥50 pro） were dramatically higher in Group Ⅲ than in Groups Ⅰ and Ⅱ at eight weeks. Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect score. Animals in Group Ⅲ presented improved cardiac regional systolic and diastolic functions as well as global systolic function in comparison with the other two groups. Conclusions： Enhanced and sustained angiogenic response can be achieved by controlled-release bFGF incorporating FG within transmyocardial laser channels, thus enabling improvement in myocardial perfusion and cardiac function.

关键词： Angiogenesis basic fibroblast growth factor Controlled release Ischemic heart disease

Destabilization of basic fibroblast growth factor by Oligodeoxynucleotides

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Chinese Chemical Letters 2006年第3期17卷 415-418页

作者： Qiang WU Ju WANG Ling ZHANG An HONG Jin Song REN Sub division of Biological Inorganic Chemistry Key Laboratory of Rare Earth Chemistry and Physics Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 Bioengineering Institute of Jinan University Guangzhou 510632

The effects of oligodeoxynucleotide （ODN） on the conformation of basic fibroblast growth factor （bFGF） were studied by spectral method. The results showed that ODN destabilized the protein.

关键词： basic fibroblast growth factor oligodeoxynucleotide (ODN) conformational changes

basic fibroblast growth factor improves learning and memory deficits in a mouse model of vascular dementia: Associated with the role of free radicals clearance?

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Neural Regeneration Research 2010年第13期5卷 1015-1019页

作者： Xian Qu Chunying Li Chang Su Bing Li Liying Wang basic Medical College of Beihua University Jilin 132013 Jilin Province China

BACKGROUND： basic fibroblast growth factor （bFGF） exhibits neuroprotective functions, but the possible mechanisms of bFGF on vascular dementia remain unclear. OBJECTIVE： To explore the neuroprotective effects of bFGF on a mouse model of vascular dementia, with focus on oxidative damage. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Medical College of Beihua University from March to December 2008. MATERIALS： bFGF was purchased from Peprotech, USA. METHODS： A total of 80 healthy, Kunming mice were randomly assigned to control, sham-surgery, model, and bFGF groups. The model and bFGF groups were used to establish vascular dementia models by repetitive cerebral ischemia-reperfusion in a conscious state. In addition, bFGF group mice were intraperitoneally injected with bFGF （100 pg/kg） following model establishment, once a day for 7 consecutive days. MAIN OUTCOME MEASURES： The Morris water maze was used to determine the influence of bFGF on learning and memory abilities in vascular dementia mice. The pathomorphological changes in hippocampal CA1 neurons were observed by Nissl staining. Superoxide dismutase and malondialdehyde changes were analyzed using biochemical analysis methods. Annexin V-FITC/PI-double-labeled flow cytometry was used to detect neuronal apoptosis. RESULTS： Learning and memory functions in model mice significantly decreased, as characterized by prolonged latency and reduced time and number of platform crossings （P 〈 0.01, P 〈 0.05）. Superoxide dismutase activity was significantly reduced, malondialdehyde content was significantly increased （P 〈 0.01）, and hippocampal neuronal apoptosis was increased （P 〈 0.01） following vascular dementia, bFGF increased superoxide dismutase activity, decreased malondialdehyde content, and reduced hippocampal neuronal apoptosis （P 〈 0.01）, which resulted in improved learning and memory in mice with vascular dementia. CONCLUSION： bFGF improved learning and memory deficits in

关键词： basic fibroblast growth factor vascular dementia Morris water maze superoxide dismutase malondialdehyde neurodegenerative disease neural regeneration

Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells

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International Journal of Ophthalmology(English edition) 2015年第5期8卷 904-909页

作者： Xin-Yi Fu Xiao-Feng Zhang Wei Xia Lei Zhong Ying-Ming Wang Zheng-Tai Sun Jing Xia Department of Ophthalmology the First Affiliated Hospital of Soochow University

AIMTo expose rat retinal Müller cells to 530 nm monochromatic light and investigate the influence of varying light illumination times on basic fibroblast growth factor (bFGF) and transforming growth factor-β1 (TGF-β1) *** groups of rat retinal Müller cells cultured in vitro under a 530 nm monochromatic light were divided into 6, 12 and 24h experimental groups, while cells incubated under dark conditions served as the control group. The bFGF and TGF-β1 mRNA expression, protein levels and fluorescence intensity of the Müller cells were *** bFGF mRNA expression and protein levels were significantly upregulated in Müller cells in all three experimental groups compared with the control group (P<0.05), while that of TGF-β1 was downregulated (P<0.05). Also, bFGF expression was positively correlated, but TGF-β1 expression was negatively correlated with illumination time. The largest changes for both cytokines were seen in the 24h group. The changes in bFGF and TGF-β1 fluorescence intensity were highest in the 24h group, and significant differences were observed among the experimental groups (P<0.05).CONCLUSIONThe expressions of bFGF and TGF-β1 changed in a time-dependent manner in Müller cells exposed to 530 nm monochromatic light with 250 lx illumination intensity. Müller cells might play a role in the development of myopia by increasing bFGF expression or decreasing TGF-β1 expression. Changes in cytokine expression in retinal Müller cells may affect monochromatic light-induced myopia.

关键词： monochromatic light myopia Mü ller cells basic fibroblast growth factor transforming growth factor-β 1