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Fine-mapping and characterisation of genes on barley(Hordeum vulgare)chromosome 2H for salinity stress tolerance during germination

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The Crop Journal 2022年第3期10卷 754-766页

作者： Edward Mwando Yong Han Tefera Angessa Xiao-Qi Zhang Chengdao Li Western Crop Genetics Alliance College of ScienceHealthEngineering and EducationMurdoch UniversityMurdochWA 6150Australia Western Australian State Agricultural Biotechnology Centre Murdoch UniversityMurdochWA 6150Australia Department of Primary Industries and Regional Development 3 Baron-Hay CourtSouth PerthWA 6151Australia

Salinity causes a detrimental impact on plant growth,particularly when the stress occurs during germination and early development stages.barley is one of the most salt-tolerant crops;previously we mapped two quantitative trait loci(QTL)for salinity tolerance during germination on the short arm of chromosome 2 H using a CM72/Gairdner doubled haploid(DH)population.Here,we narrowed down the major QTL to a region of 0.341 or 0.439 Mb containing 9 or 24 candidate genes belonging to 6 or 20 functional gene families according to barley reference genomes v1 and v3 respectively,using two DH populations of CM72/Gairdner and Skiff/CM72,F_(2)and F;generations of CM72/Gairdner/;Spartacus CL,Two Receptorlike kinase 4(RLPK4)v1 or Receptor-like kinase(RLK)v3 could be the candidates for enhanced germination under salinity stress because of their upregulated expression in salt-tolerant variety CM72.Besides,several insertion/deletion polymorphisms were identified within the 3 rd exon of the genes between CM72 and Gairdner.The sequence variations resulted in shifted functional protein domains,which may be associated with differences in salinity tolerance.Two molecular markers were designed for selecting the locus with receptor-like protein kinase 4,and one was inside HORVU2 Hr1 G111760.1 or HORVU.MOREX.r3.2 HG0202810.1.The diagnostic markers will allow for pyramiding of 2 H locus in barley varieties and facilitate genetic improvement for saline soils.Further,validation of the genes to elucidate the mechanisms involved in enhancing salinity tolerance at germination and designing RLPK4 specific markers is proposed.For this publication,all the analysis was based on barley reference genome of2017(v1),and it was used throughout for consistence.However,the positions of the markers and genes identified were updated according to new genome(v3)for reference.

关键词： barley Germination Salinity tolerance Diagnostic markers Receptor-like protein kinase 4 Gene expression Sequence analysis

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A single nucleotide substitution in the MATE transporter gene regulates plastochron and the many noded dwarf phenotype in barley(Hordeum vulgare L.)

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Journal of Integrative Agriculture 2023年第8期22卷 2295-2305页

作者： GUO Bao-jian SUN Hong-wei QI Jiang HUANG Xin-yu HONG Yi HOU Jian Lü Chao WANG Yu-lin WANG Fei-fei ZHU Juan GUO Gang-gang XU Ru-gen Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009P.R.China State Key Laboratory of barley and Yak Germplasm Resources and Genetic Improvement/Tibet Academy of Agricultural and Animal Husbandry Sciences(TAAAS) Lhasa 850002P.R.China Key Laboratory of Crop Germplasm Resources and Utilization Ministry of Agriculture/National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China

In higher plants,the shoot apical meristem produces lateral organs in a regular spacing(phyllotaxy)and timing(plastochron).The molecular analysis of mutants associated with phyllotaxy and plastochron would increase our understanding of the mechanism of shoot architecture formation.In this study,we identified mutant mnd8ynp5 that shows an increased rate of leaf emergence and a larger number of nodes in combination with a dwarfed growth habit from an EMS-treated population of the elite barley cultivar Yangnongpi 5.Using a map-based cloning strategy,the mnd8 gene was narrowed down to a 6.7-kb genomic interval on the long arm of chromosome 5H.Sequence analysis revealed that a C to T single-nucleotide mutation occurred at the first exon(position 953)of HORVU5Hr1G118820,leading to an alanine(Ala)to valine(Val)substitution at the 318th amino acid site.Next,HORVU5Hr1G118820 was defined as the candidate gene of MND8 encoding 514 amino acids and containing two multidrug and toxic compound extrusion(MATE)domains.It is highly homologous to maize Bige1and has a conserved function in the regulation of plant development by controlling the leaf initiation rate.Examination of modern barely varieties showed that Hap-1 was the dominant haplotype and was selected in barley breeding around the world.Collectively,our results indicated that mnd8ynp5 is a novel allele of the HORVU5Hr1G118820 gene that is possibly responsible for the shortened plastochron and many noded dwarf phenotype in barley.

关键词： barley EMS plastochron many noded dwarf MATE transporter shoot architecture

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Genetic analysis and gene mapping of a dwarf and liguleless mutation in barley

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The Crop Journal 2022年第4期10卷 1094-1102页

作者： Baojian Guo Jiang Qi Dongfang Li Hongwei Sun Chao Lyu Feifei Wang Juan Zhu Ganggang Guo Rugen Xu Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology Jiangsu Co-Innovation Center for Modern Production Technology of Grain CropsYangzhou UniversityYangzhou 225009JiangsuChina Joint International Research Laboratory of Agriculture and Agri-Product Safety The Ministry of Education of ChinaYangzhou UniversityYangzhou 225009JiangsuChina Key Laboratory of Crop Germplasm Resources and Utilization(MOA) The National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Leaf development underlies crop growth and productivity and has been a major target of crop domestication and improvement.However,most genes controlling leaf development in barley remain unknown.We identified a dwarf and liguleless(dl)mutant derived by ethylmethane sulfonate mutagenesis.The dl mutant showed dramatic changes in shoot architecture compared with wild-type(Yangnongpi 5)plants.Besides lacking ligules,the dl mutant showed much shorter plant height(28 cm)than Yangnongpi 5(78 cm).By map-based cloning,the dl gene was localized to a 56.58-kb genomic interval on the long arm of chromosome 7.A C-to-T single-nucleotide substitution was identified at exon position 790,and is a functional mutation resulting in a proline-to-serine substitution at the 264th amino acid residue of HORVU7Hr1G106960.Consequently,HORVU7Hr1G106960 was identified as the DL gene,encoding 269 amino acids and containing the Arabidopsis LSH1 and Oryza G1(ALOG)domain.DL is highly similar to rice OsG1-LIKE 1/2(OsG1L1/2)and sorghum AWN1/AWN1-10 at the amino acid level.Although the dl mutant allele showed no expression changes in selected tissues by real-time PCR,we propose HORVU7Hr1G106960 as a candidate gene conferring the dwarf and liguleless phenotype in barley.

关键词： barley EMS Liguleless ALOG Shoot architecture

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Salicylic Acid Application Mitigates Oxidative Damage and Improves the Growth Performance of barley under Drought Stress

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Phyton-International Journal of Experimental Botany 2023年第5期92卷 1513-1537页

作者： Shah Mohammad Naimul Islam Niloy Paul Md.Mezanur Rahman Md.Ashraful Haque Md.Motiar Rohman Mohammad Golam Mostofa Institute of Biotechnology and Genetic Engineering Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur1706Bangladesh Institute of Genomics for Crop Abiotic Stress Tolerance Department of Plant and Soil ScienceTexas Tech UniversityLubbock79409USA Plant Breeding Division Bangladesh Agricultural Research InstituteGazipur1701Bangladesh Department of Energy Plant Research Laboratory Michigan State UniversityEast Lansing48824USA

Drought is a severe environmental constraint,causing a significant reduction in crop productivity across the world.Salicylic acid(SA)is an important plant growth regulator that helps plants cope with the adverse effects induced by various abiotic stresses.The current study investigated the potential effects of SA on drought tolerance efficacy in two barley(Hordeum vulgare)genotypes,namely BARI barley 5 and BARI barley 7.Ten-day-old barley seedlings were exposed to drought stress by maintaining 7.5%soil moisture content in the absence or presence of 0.5,1.0 and 1.5 mM SA.Drought exposure led to severe damage to both genotypes,as indicated by phenotypic aberrations and reduction of dry biomass.On the other hand,the application of SA to drought-stressed plants protected both barley genotypes from the adverse effects of drought,which was reflected in the improvement of phenotypes and biomass production.SA supplementation improved relative water content and proline levels in drought-stressed barley genotypes,indicating the osmotic adjustment functions of SA under water-deficit conditions.Drought stress induced the accumulation of reactive oxygen species(ROS),such as hydrogen peroxide(H2O2)and superoxide(O_(2)•^(−)),and the lipid peroxidation product malondialdehyde(MDA)in the leaves of barley plants.Exogenous supply of SA reduced oxidative damage by restricting the accumulation of ROS through the stimulation of the activities of key antioxidant enzymes,including superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),ascorbate peroxidase(APX)and glutathione peroxidase(GPX).Among the three-applied concentrations of SA,0.5 mM SA exhibited better mitigating effects against drought stress considering the phenotypic performance and biochemical data.Furthermore,BARI barley 5 showed better performance under drought stress than BARI barley 7 in the presence of SA application.Collectively,our results suggest that SA played a crucial role in improving water status and antioxidant defense strategy to protect barley plants from t

关键词： Antioxidant defense barley drought stress plant growth reactive oxygen species salicylic acid

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Effects of Rate and Source Organo-Mineral Material on Forage Yield and Nutritive Value of barley-Pea Mixed under Arid Conditions

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1483-1493页

作者： Walid Soufan Nasser A.Al-Suhaibani Plant Production Department College of Food and Agriculture SciencesKing Saud UniversityRiyadh11451Saudi Arabia

Cereal-legume intercropping plays a vital role in the subsistence food production system that prevails in the arid regions.It not only provides profitable crop productivity for agricultural communities but also plays an important role in improving soil fertility.Therefore,the present research was conducted to assess the effect of the organic and mineral fertilizers on the forage yield and nutritional value in barley-pea intercropping system.The results revealed that the quality of forage grass is significantly influenced by both organic and inorganic fertilizer.Thus,organic fertilizer application has significantly influenced the dry matter(DM),crude ash(CA),crude protein(CP),neutral detergent fiber(NDF),and acid detergent fiber(ADF).Among the treatments,organic fertilizer added at rate of 35 m^(−3) ha^(−1) produced the maximum fresh and dry matter in barley and peas.In addition,the same level of organic fertilizer also improved silage composition by significantly increasing the protein and fiber content and showed highest the values.Based on overall results,it is concluded that organic fertilizer treatment(35 m^(−3) ha^(−1))has the potential as an effective strategy to improve the productivity and nutritional quality of the barley-pea intercropping system in arid areas.The results revealed that organic fertilizer can be utilized in sustainable agricultural as a source of nutrients for numerous various crops under arid conditions.

关键词： Organic fertilizer barley pea yield quality hay silage

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Transcriptome Profiling of barley Cultivar Hua 30 MDEC in Response to Agrobacterium Infection

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Phyton-International Journal of Experimental Botany 2022年第6期91卷 1153-1164页

作者： Yingbo Li Ting He Guimei Guo Hongwei Xu Yingjie Zong Shuwei Zhang Ruiju Lu Longhua Zhou Chenghong Liu Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai201403China Shanghai Key Laboratory of Agricultural Genetics and Breeding Shanghai201106China

Agrobacterium-mediated transformation has been widely used in plants.However,the mechanism in plant cells’response to Agrobacterium infection was very complex.The mechanism of the determinants in host cell remains obscure,especially in barley,which is recalcitrant for Agrobacterium-mediated transformation.In the present study,microspore-derived embryogenic calli(MDEC)from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process.Hua 30 MDEC can be successfully infected by Agrobacterium.RNA-sequencing at different infection points(0,2,6,12,24 hpi)was performed.The average expressional intensity of the whole genomics increased from 0 to 2 hpi,and then decreased subsequently.More upregulated than downregulated differentially expressed genes(DEGs)were counted at the same time.GO enrichment analysis showed that protein modification was significantly overrepresented in upregulated DEGs.Chromosome-related biological processes,gene expression and cellular metabolic processes were significantly overrepresented in downregulated DEGs.KEGG analysis showed that plant defense responses,phenylpropanoid biosynthesis and biosynthesis of amino acids were significantly enriched across the infection time course.Nine DEGs related to defense responses were identified.All DEGs were upregulated from 2 to 24 hpi.We speculate that these genes are possibly related to Agrobacterium infection.These findings will provide deep insights into the molecular events occurring during the process of Agrobacterium-mediated transformation.

关键词： barley Agrobacterium-mediated transformation microspore-derived embryogenic callus transcriptome analysis

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Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid,Diploid and Tetraploid barley Seedlings Derived from Microspore Culture

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1419-1428页

作者： Longhua Zhou Ting He Jing Li Guimei Guo Yingbo Li Hongwei Xu Runhong Gao Linli Huang Yifei Wang Ruiju Lu Zhiwei Chen Chenghong Liu Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai200000China Biotechnology Research Institute Shanghai Key Laboratory of Agricultural Genetics and BreedingShanghai200000China Shanghai Baixin Biotechnology Company Limited Shanghai200000China

Phytohormones play important roles in plant growth and development,and polyploids are thought to be an important method for plant breeding.However,the relationship between ploidy and phytohormone is still unclear.In this study,barley at three ploidy levels were produced by microspore culture.Therefore,we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution.The results showed that Abscisic acid(ABA),gibberellin(GA),jasmonic acid(JA),auxin(IAA),salicylic acid(SA)and cytokinin(CTK)were successfully determined in shoots and roots using LC-MS(liquid chromatography mass spectrometry).By comparing the shoots of the haploid and diploid plants,it was found that the distribution trend of the six phytohormones was consistent,and another consistent trend was found in the roots of the diploid and tetraploid plants.In addition,we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid,diploid and tetraploid.Here,the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.

关键词： barley polyploidy microspore culture phytohormone shoot root

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Construction of the Seed-Coat cDNA Microarray and Screening of Differentially Expressed Genes in barley

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Acta Biochimica et Biophysica Sinica 2004年第10期36卷 695-700页

作者： Jin-Song PANG*, Meng-Yuan HE, and Bao LIU Laboratory of Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China Laboratory of Molecular Epigenetics Institute of Genetics and Cytology Northeast Normal UniversityChangchun 130024 China

Some barley mutants can synthesize neither anthocyanins nor proanthocyanidins in the seed coat, which is related to several genes in locus Ant13, but the exact model of action remains unknown. We used the cDNA microarray technology with barley transcription-deficient mutant (ant13-152) that does not synthesize proanthocyanidins as the tester, and its wild type genotype (Triumph) as the driver, to study this question. Six-thousand and forty-eight clones from the wild type Morex testa+pericarp cDNA library were amplified using PCR, and the DNA fragments were spotted on commercial amino-modified glass slide as microarray. The mRNAs from the developing seed coat (8–15 days) of both the mutant and the wild-type barley plants were isolated, and labeled respectively with Cy3-dUTP and Cy5-dUTP when reversely trans- cribed to cDNAs. The labeled cDNAs were used as probes, mixed at the same molar concentration, and hybridized with the DNA fragments on the slide. Seventy clones exhibiting marked differential expression (ratio>4) were identified from the microarray. All the 25 cDNA clones that showed an over-expression in wild type in comparison to the mutant ant13-152 were sequenced. It was found that most of these over- expressing clones were transcription/translation and hordein-associated genes. These results have laid a solid material basis for further elucidation of the metabolic pathway in proanthocyanidin synthesis in barley and likely other plants.

关键词： barley seed coat proanthocyanidin cDNA microarray

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barley grain for ruminants: A global treasure or tragedy

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Journal of Animal Science and Biotechnology 2012年第4期3卷 167-175页

作者： Akbar Nikkhah Department of Animal Sciences Faculty of Agricultural Sciences University of Zanjan

barley grain （Hordeum vulgate L.） is characterized by a thick fibrous coat, a high level of β-glucans and simply-arranged starch granules. World production of barley is about 30 % of that of corn. In comparison with corn, barley has more protein, methionine, lysine, cysteine and tryptophan. For ruminants, barley is the third most readily degradable cereal behind oats and wheat. Due to its more rapid starch fermentation rate compared with corn, barley also provides a more synchronous release of energy and nitrogen, thereby improving microbial nutrient assimilation. As a result, feeding barley can reduce the need for feeding protected protein sources. However, this benefit is only realized if rumen acidity is maintained within an optimal range （e.g., 〉 5.8 to 6.0）; below this range, microbial maintenance requirements and wastage increase. With a low pH, microbial endotoxines cause pro-inflammatory responses that can weaken immunity and shorten animal longevity. Thus, mismanagement in barley processing and feeding may make a tragedy from this treasure or pearl of cereal grains. Steam-rolling of barley may improve feed efficiency and post-rumen starch digestion. However, it is doubtful if such processing can improve milk production and feed intake. Due to the need to process barley less extensively than other cereals （as long as the pericarp is broken）, consistent and global standards for feeding and processing barley could be feasibly established. In high-starch diets, barley feeding reduces the need for capacious small intestinal starch assimilation, subsequently reducing hindgut starch use and fecal nutrient loss. With its nutritional exclusivities underlined, barley use will be a factual art that can either matchlessly profit or harm rumen microbes, cattle production, farm economics and the environment.

关键词： barley Cereal Ruminant Starch Treasure

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The effect of Tyrophagus putrescentiae on Fusarium poae transmission and fungal community in stored barley in a laboratory experiment

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Insect Science 2014年第1期21卷 65-73页

作者： Jan Hubert Marta Nesvorna Jan Kopecky Crop Research Institute Praha 6 Czech Repubfic

The effect of Tyrophagus putrescentiae on Fusarium poae transmission and fungal community composition was studied in nonsterile barley grain. The experiments included following treatments： control barley without mites; barley containing l0 or 50 mites without preincubation on E poae （Tpl0 and TpS0）; barley containing 10 or 50 mites after preincubation on E poae （FTp 10 and FTp50）. The number of mites, suc cessful transfer of E poae, and changes in the fungal communities were examined after 21 d of experiment. Increase of deoxynivalenol （DON） content in the barley was chosen as a criterion of successful Epoae transfer. The preincubation of T. putrescentiae on Epoae increased DON level approximately to 800 and 300μg/kg of grain for FTpl0 and FTpS0, respectively. T. putrescentiae population growth in FTpl0 was lower than in Tpl0, while no difference was found between FTp50 and Tp50. Fungal communities were compared by amplification, cloning and sequencing of ITS fragments, and operational taxonomic units （OTU） analysis. The OTU analysis did not support the transfer ofF. poae via mites. From the analyzed clones, only 13 cloned sequences clustered with E poae in an OTU defined at distance level 0.07. The related clones originated from FTpl0, Tpl 0, Tp50 and control treatments, but not from FTp50. However, the presence of E poae in FTp50 was confirmed by PCR amplification with specific primers. The observation may be explained by different effect of mite population density, that is, in the high density, （FTp50 treatment） the fungus was overgrazed, while the lower population density （FTp 10） supported E poae transfer.

关键词： barley deoxinivalenol （DON） Fusariumpoae mites mycotoxin storage

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