The effect of Tyrophagus putrescentiae on Fusarium poae transmission and fungal community in stored barley in a laboratory experiment
The effect of Tyrophagus putrescentiae on Fusarium poae transmission and fungal community in stored barley in a laboratory experiment作者机构：Crop Research Institute Praha 6 Czech Repubfic
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2014年第21卷第1期
页 面：65-73页
核心收录：
学科分类：0710[理学-生物学] 0820[工学-石油与天然气工程] 082001[工学-油气井工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学]
主 题：barley deoxinivalenol (DON) Fusariumpoae mites mycotoxin storage
摘 要：The effect of Tyrophagus putrescentiae on Fusarium poae transmission and fungal community composition was studied in nonsterile barley grain. The experiments included following treatments： control barley without mites; barley containing l0 or 50 mites without preincubation on E poae （Tpl0 and TpS0）; barley containing 10 or 50 mites after preincubation on E poae （FTp 10 and FTp50）. The number of mites, suc cessful transfer of E poae, and changes in the fungal communities were examined after 21 d of experiment. Increase of deoxynivalenol （DON） content in the barley was chosen as a criterion of successful Epoae transfer. The preincubation of T. putrescentiae on Epoae increased DON level approximately to 800 and 300μg/kg of grain for FTpl0 and FTpS0, respectively. T. putrescentiae population growth in FTpl0 was lower than in Tpl0, while no difference was found between FTp50 and Tp50. Fungal communities were compared by amplification, cloning and sequencing of ITS fragments, and operational taxonomic units （OTU） analysis. The OTU analysis did not support the transfer ofF. poae via mites. From the analyzed clones, only 13 cloned sequences clustered with E poae in an OTU defined at distance level 0.07. The related clones originated from FTpl0, Tpl 0, Tp50 and control treatments, but not from FTp50. However, the presence of E poae in FTp50 was confirmed by PCR amplification with specific primers. The observation may be explained by different effect of mite population density, that is, in the high density, （FTp50 treatment） the fungus was overgrazed, while the lower population density （FTp 10） supported E poae transfer.
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