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Screening and degrading characteristics and community structure of a high molecular weight polycyclic aromatic hydrocarbon-degrading bacterial consortium from contaminated soil

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Journal of Environmental Sciences 2010年第10期22卷 1576-1585页

作者： Run Sun Jinghua Jin Guangdong Sun Ying Liu Zhipei Liu State Key Laboratory of Microbial Resources Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China. Environmental Protection Research Institute of Light Industry Beijing 100089 China

Inoculation with efficient microbes had been proved to be the most important way for the bioremediation of polluted environments. For the treatment of abandoned site of Beijing Coking Chemical Plant contaminated with high level of high-molecular-weight polycyclic aromatic hydrocarbons （HMW-PAHs）, a bacterial consortium capable of degrading HMW-PAHs, designated 1-18-1, was enriched and screened from HMW-PAHs contaminated soil. Its degrading ability was analyzed by high performance liquid chromatography （HPLC）, and the community structure was investigated by construction and analyses of the 16S rRNA gene clone libraries （A, B and F） at different transfers. The results indicated that 1-18-1 was able to utilize pyrene, fluoranthene and benzo[a]pyrene as sole carbon and energy source for growth. The degradation rate of pyrene and fluoranthene reached 82.8% and 96.2% after incubation for 8 days at 30℃, respectively; while the degradation rate of benzo[a]pyrene was only 65.1% after incubation for 28 days at 30℃. Totally, 108, 100 and 100 valid clones were randomly selected and sequenced from the libraries A, B, and E Phylogenetic analyses showed that all the clones could be divided into 5 groups, Bacteroidetes, ct-Proteobacteria, Actinobacteria, β-Proteobacteda and γ- Proteobacteria. Sequence similarity analyses showed total 39 operational taxonomic units （OTUs） in the libraries. The predominant bacterial groups were α-Proteobacteria （19 OTUs, 48.7%）, γ-Proteobacteria （90TUs, 23.1%） and β-Proteobacteria （80TUs, 20.5%）. During the transfer process, the proportions of α-Proteobacteria and β-Proteobacteria increased greatly （from 47% to 93%）, while γ-Proteobacteda decreased from 32% （library A） to 6% （library F）; and Bacteroidetes group disappeared in libraries B and F.

关键词： high-molecular-weight-PAHs bacterial consortium bacterial community structure 16S rRNA gene library

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Growth Kinetics Study of a bacterial consortium Producing Biosurfactants,Constructed with Six Strains Isolated from an Oily Sludge

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Advances in Bioscience and Biotechnology 2014年第5期5卷 418-425页

作者： Zohra Sadouk-Hachaichi Ammal Tazerouti Hocine Hacene Laboratory of Applied Organic Chemistry Faculty of ChemistryUniversity of Sciences and Technology H.Boumediène(USTHB)AlgiersAlgeria Laboratory of Microbiology Faculty of Biological SciencesUniversity of Sciences and Technology H.Boumediène(USTHB)AlgiersAlgeria

We investigated the ability of a bacterial community constructed with six strains isolated from an oily sludge, to utilize diesel oil at high concentrations. The consortium was able to grow at concentrations up to 84 g diesel oil/L and had produced biosurfactants during its active growth phase;these compounds, in their crude form, reduced the surface tension of distilled water from 72 mN/m to 31 mN/m, with a corresponding Critical Micelle Concentration value γCMC = 81 mg/L. The plot of specific growth rates obtained from the growth curves versus initial concentrations was found to fit adequately the experimental data by the Andrews inhibitory model, which resulted in the following kinetic constants: μmax = 0.535d-1 ± 0.063, KS = 18.68 g/L ± 3.59 and KI = 29.02 g/L ± 4.96, reflecting the slow biodegradation rate. At 25.2 g diesel oil/L, close to the optimal concentration S* = 23.28 g/L ± 4.23, the consortium metabolized diesel oil faster than each strain did individually, suggesting that the process was stimulated by a synergistic interaction between the members of the consortium.

关键词： Andrews Model bacterial consortium Biodegradation Biosurfactants Diesel Oil Kinetic Parameters

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Unraveling the skatole biodegradation process in an enrichment consortium using integrated omics and culture-dependent strategies

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Journal of Environmental Sciences 2023年第5期127卷 688-699页

作者： Qiao Ma Nan Meng Jiancheng Su Yujie Li Jiazheng Gu Yidi Wang Jingwei Wang Yuanyuan Qu Zelong Zhao Yeqing Sun Institute of Environmental Systems Biology College of Environmental Science and EngineeringDalian Maritime UniversityDalian 116026China Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic Animals Liaoning Ocean and Fisheries Science Research InstituteDalian 116023China Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education) School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China

3-Methylindole(skatole)is regarded as one of the most offensive compounds in odor *** is feasible for skatole removal but the functional species and genes responsible for skatole degradation remain *** this study,an efficient aerobic skatole-degrading consortium was *** and Pseudomonas were identified as the two major and active populations by integrated metagenomic and metatranscriptomic *** analyses indicated that the skatole downstream degradation wasmainly via the catechol pathway,and upstream degradation was likely catalyzed by the aromatic ring-hydroxylating oxygenase and flavin *** binning and gene analyses indicated that Pseudomonas,Pseudoclavibacter,and Raineyella should cooperate with Rhodococcus for the skatole degradation ***,a pure strain Rhodococcus ***1 was successfully obtained which could utilize skatole as the sole carbon *** genome sequencing showed that strain DMU1 was the predominant population in the *** crude enzyme and RT-qPCR assays indicated that strain DMU1 degraded skatole through the catechol ortho-cleavage ***,our results suggested that synergistic degradation of skatole in the consortium should be performed by diverse bacteria with Rhodococcus as the primary degrader,and the degradation mainly proceeded via the catechol pathway.

关键词： Skatole Biodegradation bacterial consortium Metagenomics and metatranscriptomics Rhodococcus

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