Unraveling the skatole biodegradation process in an enrichment consortium using integrated omics and culture-dependent strategies

作     者：Qiao Ma Nan Meng Jiancheng Su Yujie Li Jiazheng Gu Yidi Wang Jingwei Wang Yuanyuan Qu Zelong Zhao Yeqing Sun Qiao Ma;Nan Meng;Jiancheng Su;Yujie Li;Jiazheng Gu;Yidi Wang;Jingwei Wang;Yuanyuan Qu;Zelong Zhao;Yeqing Sun

作者机构：Institute of Environmental Systems BiologyCollege of Environmental Science and EngineeringDalian Maritime UniversityDalian 116026China Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic AnimalsLiaoning Ocean and Fisheries Science Research InstituteDalian 116023China Key Laboratory of Industrial Ecology and Environmental Engineering(Ministry of Education)School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第127卷第5期

页      面：688-699页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 0903[农学-农业资源与环境] 0703[理学-化学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(No.32170121) the Fundamental Research Funds for the Central Universities(No.3132022159) 

主　　题：Skatole Biodegradation Bacterial consortium Metagenomics and metatranscriptomics Rhodococcus 

摘      要：3-Methylindole(skatole)is regarded as one of the most offensive compounds in odor *** is feasible for skatole removal but the functional species and genes responsible for skatole degradation remain *** this study,an efficient aerobic skatole-degrading consortium was *** and Pseudomonas were identified as the two major and active populations by integrated metagenomic and metatranscriptomic *** analyses indicated that the skatole downstream degradation wasmainly via the catechol pathway,and upstream degradation was likely catalyzed by the aromatic ring-hydroxylating oxygenase and flavin *** binning and gene analyses indicated that Pseudomonas,Pseudoclavibacter,and Raineyella should cooperate with Rhodococcus for the skatole degradation ***,a pure strain Rhodococcus ***1 was successfully obtained which could utilize skatole as the sole carbon *** genome sequencing showed that strain DMU1 was the predominant population in the *** crude enzyme and RT-qPCR assays indicated that strain DMU1 degraded skatole through the catechol ortho-cleavage ***,our results suggested that synergistic degradation of skatole in the consortium should be performed by diverse bacteria with Rhodococcus as the primary degrader,and the degradation mainly proceeded via the catechol pathway.