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ASK1 physically interacts with COI1 and is required for male fertility in arabidopsis

Science China(Life Sciences) 2002年第6期45卷 631-636页

作者：戴良英李栒罗宽徐领会官春云黄大昉 College of Plant Science and Technology Hunan Agricultural University Changsha 410128 China Institute of Biotechnology Research Chinese Academy of Agricultural Sciences Beijing 100081 China

Jasmonates are a new class of plant hormones that play important roles in plant development and plant defense. The COI1 gene was previously shown to be required for jasmonate- regulated plant fertility and defense. We demonstrated for the first time that COI1 interacts with the arabidopsis.SKP1-LIKE1 (ASK1) to form a complex that is required for jasmonate action in planta. Functional analysis by antisense strategy showed that ASK1 is involved in male fertility.

关键词： ASK1, COI1, SCF, ubiquitin ligase, arabidopsis.

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arabidopsis.gene URO respond to the light signal and effect the auxin signal network

Arabidopsis gene URO respond to the light signal and effect ...

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中国科学院植物分子生物学前沿国际研讨会

作者： Yue Sun Zheng Yuan Wei Zhou Ben Xu Xiaofang Li Hai Huang life science college of the East China Normal University North Zhongshan Road 3663 Shanghai China 200062 Shanghai Institute of Plant Physiology and Ecology of CAS Fenglin Road 300 Shanghai China 200032

＜正＞From arabidopsis.T-DNA insertion mutagenesis population, we selected a semi-dominant mutant named wo, with some phenotype that always connected with auxin signal faults, such as lack of apical dominance, cup-like shoots and poorly lignin synthesis. We have cloned the URO （up right rosette） gene. Computer predicts that it is an unknown transcription factor with a C2H2 zinc finger domain. As the proof that URO gene connected with auxin signal pathway, it can promote the auxin response

关键词： auxin light URO gene arabidopsis.

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KD127 Plays Important Roles in Pollen Tube and Vegetative Growth in arabidopsis.thaliana

KD127 Plays Important Roles in Pollen Tube and Vegetative Gr...

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2006年拟南芥研究学术研讨会

作者： Ke-Zhen Yang, Xiao-Lei Liu, Chuan Xia, Meng-Meng Zhu, Li-Qun Chen, Xue-Qin Zhang, and De Ye State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University Yuanmingyuan Xilu, Beijing 100094, China.

＜正＞To understand the genetic mechanisms that regulate the formation and development of plant male gametophytes, we are performing a large scale characterization of male gametophytic mutants in arabidopsis. Here we report the characterization of a novel mutant kd127 that was defective in pollen tube growth. The kd127 mutant was identified by its distorted kanamycin-resistance segregation in a

关键词： KD127 pollen sexual reproduction cell signaling cell patterning arabidopsis.

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The molecular mechanism of thermo-tolerance in arabidopsis.pollen tubes and thermo-sensitive male sterility

The molecular mechanism of thermo-tolerance in Arabidopsis p...

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第六届中国植物逆境生理学与分子生物学学术研讨会

作者： Ke-Zhen Yang,Chuan Xia,Xiao-Lei Liu,Xiao-Ying Dou,Zhao-Xia Ma,Li-Qun Chen,Xue-Qin Zhang,and De YeState Key Laboratory of Plant Physiology and Biochemistry,College of Biological Sciences,China Agricultural University,Yuanmingyuan Xilu 2,Beijing 100193,China

＜正＞In most flowering plant species,pollination and fertilization occur during the hot summer.The plants must have evolved a mechanism that ensures normal growth of their pollen tubes at high temperatures.Disruption... 详细信息

关键词： Male gametophyte Pollen tube Pollination Heat stress Heat shock protein arabidopsis.

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Control of cell pluripotency in plants

Control of cell pluripotency in plants

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从植物科学到农业发展——2012全国植物生物学大会

作者： Mingzhu Fan,Chongyi Xu,Ke Xu,Wei Xin,Jing Liu and Yuxin Hu~* Key Laboratory of Plant Molecular Physiology,Institute of Botany,Chinese Academy of Sciences, Beijing 100093.

Plant cells have long been considered to be pluripotent because most of cells can regenerate a whole plant under appropriate culture conditions.Although the remarkable regeneration capability of plant tissues or organs under the culture conditions has underlain an extensive practice for decades,the early molecular mechanism underlying callus initiation is largely elusive.We analyzed the dynamic transcriptome profiling of callus initiation in arabidopsis.aerial and root explants,and identified 1,342 differentially expressed genes in both explants after incubation on callus inducing medium(CIM).These genes were mainly related to hormone homeostasis and signaling,transcriptional and post transcriptional regulations, protein phosphorelay cascades and DNA- or chromatin-modification.We further showed that four LATERAL ORGAN BOUNDARIES DOMAIN(LBD)/ASYMMETRIC LEAVES2-LIKE(ASL) downstream of AUXIN RESPONSE FACTORs(ARFs),LBD16, LBD17,LBD18 and LBD29,were rapidly and dramatically induced by CIM in multiple organs.Ectopic expression of each of the four LBD genes in arabidopsis.was sufficient to trigger the spontaneous callus formation without exogenous phytohormone,whereas suppression of LBD function inhibited the callus formation induced by CIM.We also provided the evidence that the callus directed by LBD resembled that induced by CIM by characteristics of ectopically activated root meristem genes and efficient regeneration capacity.Our work defines LBD transcription factors as key regulators in callus induction process,thereby establishing a molecular link between auxin signaling and cell pluripotency control in plants.

关键词： LBD Callus formation,Auxin Cell pluripotency arabidopsis.

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