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Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe

Food Science and Human Wellness 2023年第4期12卷 1257-1264页

作者： Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China

Food safety is a major issue to public health and have attracted global ***,sensitive,and reliable detection methods for food hazardous substances is highly *** which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances,which play an important role in the development and application of new food safety detection *** current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ***,we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer,which is immobilized-free under ambient *** design employs the Le Chatelier’s principle and could be used to effectively measure small molecule-aptamer *** binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity,in which their dissociation equilibria measurements are in excellent close to their previous *** to the simplicity and sensitivity of this new method,we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.

关键词： Aptamer Small molecule Dissociation equilibria antisense displacement probe Le Chatelier’s principle

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