Investigation on small molecule-aptamer dissociation equilibria based on antisense displacement probe

作     者：Lei Wang Lili Yao Qihui Ma Yu Mao Hao Qu Lei Zheng Lei Wang;Lili Yao;Qihui Ma;Yu Mao;Hao Qu;Lei Zheng

作者机构：School of Food and Biological EngineeringHefei University of TechnologyHefei 230009China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第4期

页      面：1257-1264页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 

基　　金：supported by the National Key R&D Program of China(2017YFC1600603) the Funds for Huangshan Professorship of Hefei University of Technology(407-037019) 

主　　题：Aptamer Small molecule Dissociation equilibria Antisense displacement probe Le Chatelier’s principle 

摘      要：Food safety is a major issue to public health and have attracted global ***,sensitive,and reliable detection methods for food hazardous substances is highly *** which can bind to the target molecules with high affinity and specificity represent an attractive tool for the recognition of food hazardous substances,which play an important role in the development and application of new food safety detection *** current assays for characterizing small molecule-aptamer binding are limited by either the mass sensitivity or the size differentiation ***,we proposed a comprehensive method for assessing the dissociation equilibria of small molecule-aptamer,which is immobilized-free under ambient *** design employs the Le Chatelier’s principle and could be used to effectively measure small molecule-aptamer *** binding aptamer and anti-aflatoxin B1 aptamer were used as the model system to determine their affinity,in which their dissociation equilibria measurements are in excellent close to their previous *** to the simplicity and sensitivity of this new method,we believe that it could be recommended as an effective tool for characterizing small molecule-aptamer interactions and promote the further application of small molecular aptamer in food safety.