检索结果-南通市图书馆

Inhibition of Cell Proliferation and Cell Cycle Progression by Antisense Inhibition of CIC-3 Chloride Channels in Nasopharyngeal Carcinoma Cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibition of Cell Proliferation and Cell Cycle Progression ...

Effects of CDK4 Antisense RNA on Human breast cancer cell proliferation and expression of cyclinD1,cyclinE and CDK2

中国细胞生物学学会第九次会员代表大会暨青年学术大会

作者： Jian-Wen Mao~1 Bin Xu~1 Li-Wei Wang~2 Li-Xin Chen~2 Li-Jing Wang~1 Hong-Zhi Li~1 Jiao Guo~1 Wei-Dong Li~1 1 Institute of Basic Medical Sciences and Department of Biology,Guangdong Pharmaceutical University,China 2 Department of Physiology,Medical College,Jinan University,China

Activation of the volume-activated chloride channel has been suggested to play a role in key elements of tumor development including proliferation,migration,invasiveness,and angiogenesis.In present study,the Antisense‘knock- out’strategy was used to investigate the role and the underlying mechanism of C1C-3,the most likely molecular

关键词： ClC-3 Antisense cell proliferation cell cycle chloride channels volume regulation

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1999年第6期44卷 541-544页

作者： SANG Jianli WANG YongchaoLaboratory of Cell Biology, Department of Biology, Beijing Normal University, Beijing 100875, China Laboratory of Cell Biology Department of Biology Beijing Normal University Beijing China

The role of CDK4 in human breast cancer cell proliferation and expression of cyclinD1, cyclinE and CDK2 has been investigated using inhibition of CDK4 expression by Antisense RNA. When CDK4 expression was inhibited, the rate of cell proliferation and tumorigenecity decreased apparently.This indicates that CDK4 plays an important role in formation and development of breast tumor. The resuits of Northern blot analysis showed that the levels of cyclinD1 and CDK2 mRNAs changed slightly whereas the level of cyclinE mRNA decreased obviously. It is suggested that the expression of CDK4 is necessary for induction of cyclinE expression. Thus, inhibition of CDK4 expression affects not only the role of CDK4 itself but also the role of other genes.

关键词： CDK4 breast cancer cell Antisense RNA cyclinE.

Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by Antisense technology

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1999年第6期44卷 533-537页

作者： Wenwei Hu Yingnian Yu Xingruo Chen Tao Song Haiyang Xie Zhejiang Med Univ Dept Pathophysiol Hangzhou 310031 Peoples R China

Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyI-N’-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting Antisense RNA, MNNGinduced nontargeted mutation frequency enhanced significantly compared with control ( P < 0.05). The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.

关键词： Antisense RNA mutation nontargeted genetic instability.

RETROVIRUS MEDIATED TRANSFER OF Antisense HUMAN c-myc GENE INTO HUMAN ESOPHAGEAL CANCER CELLS SUPPRESSED CELL PROLIFERATION AND MALIGNANCY

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1992年第1期35卷 76-83页

作者：叶昕吴旻 National Laboratory of Molecular Ontology Cancer Institute Chinese Academy of Medical Sciences Beijing 100021 PRC

A retroviral vector, called pDAM3, containing the neomycin resistant gene and the Antisense human c-myc gene fragment (the third exon and 3’flanking sequence) was constructed. pDAM3 was introduced into amphotropic packaging cells PA317 by the calcium phosphate precipitation method. Several G418-resistant PA317 clones were isolated. The virus titer of these cell lines was determined by infectivity of their cuhure fluid to NIH/3T3 cells. The highest titer obtained was 8×10~5 G418-resistant colony forming units/ml. Clonal and pooled G418-resistant PA317 colonies with high titers were expanded and analyzed by Southern blot for the presence of intact viral sequences. All cell lines were found to harbor the internal sequences of the pDAM3 vector without any rearrangement. Recombinant virus DAM3 infected human esophageal cancer cell line EC8712 efficiently. The DAM3-infected EC8712 (called EC-DAM3) was found to contain the full DAM3 sequence (4.8kb) by Southern blot analysis. Antisense myc RNA expressed in

关键词： retroviral vector myc oncogene Antisense RNA esophageal cancer cell.

The effects of Antisense PTEN gene transfection on the growth and invasion of glioma cells

在线全文

学校读者我要写书评

暂无评论

Journal of Medical Colleges of PLA(China) 2006年第5期21卷 307-311页

作者：陈宏颉郑兆聪王如密王手森杨卫忠 Department of Neurosurgery Fuzhou General Hospital

Objective:To study the effects of Antisense PTEN gene on the growth and invasion of glioma cells. Methods:A pcDNA3. 1/Hygro (-) recombinant plasmid containing Antisense PTEN gene fragment was constructed. Glioma cells of primary culture were transfected with Antisense PTEN gene vector and stably transfected clones were selected. Then, the different growth and invasion abilities and the different MMP9 mRNA expressions of three kinds of cells were observed, including the transfected cells, untransfected cells and the cells transfected with empty vector. Results :The abilities of growth and invasion of the transfected cells and the expressions of MMP9 mRNA were obviously enhanced. Conclusion: Antisense PTEN gene could have a negative impact on the growth and invasion of primary culture glioma cells.

关键词： PTEN gene glioma invasion Antisense

Abrupt reduction of c-myc expression by Antisense RNA inducing terminal differentiation and apoptosis of a human esophageal cancer cell line

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1995年第5期38卷 580-589页

作者：赵晓航王秀琴周传农彭仁玲阎水中吴旻 (State Key Laboratory of Molecular Oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100021 China)

A human esophageal cancer cell line (EC8712) expressing high-level Myc protein was infected with recombmant retroviral particles (pA-BD9) at a multiplicity of infection (MOI) 1:1. This viral particle contains a neomycin-resistant gene and a 1.53-kb Antisense RNA spanning the 2nd exon and its flanking sequences of the human c-myc oncogene. The G418-resistant EC8712 clones showed an 86% inhibition of growth rate and morphological changes characteristic of terminal differentiation and apoptosis. A decrease of about 80% of Myc protein was also observed in these infected cells by ABC-ELISA assay. 12-24 h after the infection of ECS712 cells with pA-BD9 at a high viral particle concentration (MOI = 1:10), the integration of the extrinsic 1.53-kb Antisense c-myc fragment into the cancer cell genome was evidenced by the Southern blot analysis. Northern blot analyses showed the expression of this Antisense fragment and a decrease of the intrinsic c-myc expression by 74% in comparison with that of the parental

关键词： c-myc gene Antisense RNA esophageal cancer differentiation apoptosis.

Reversal of drug resistance in multidrug-resistant human lung cancer cells by retrovirus-mediated MDR1 Antisense RNA

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1996年第23期41卷 1996-2000页

作者：高振强高志萍 Department of Pathology Beijing Medical University Beijing 100083 China

Human lung cancer is one of the commonest cancers in the world,and its incidenceand mortality are very high.About 50% of the patients with lung cancer can be treatedwith surgery,and another 50% need chemotherapy because of cancer metastasis.Addition-ally the patients already having surgical treatment often need chemotherapy.However,thechemotherapy often loses its function on these patients,especially in non-small-cell

关键词： lung cancer reversal of drug resistance MDR1 Antisense RNA retrovirus.

THE Antisense C-HA-RAS OLIGODEOXY-NUCLEOTIDES INHIBIT THE PROLIFERATION OF THE GASTROCARCINOMA-DNA-TRANSFORMED RAT 3-3 CELLS AND REDUCE THE P21 EXPRESSION OF THE CELLS

在线全文

学校读者我要写书评

暂无评论

Science China Chemistry 1991年第1期34卷 35-41页

作者：许毅邓国仁梁亚云李赤 Biochemistry Department Beijing Institute for Cancer Research Beijing 100034 PRC

The Rat 3-3 is a secondary transformant of the rat fibroblast cell line (Rat-1) transfectedwith total DNA of a gastrocarcinoma cell line BGC-823. The cells over-express the c-Ha-rasoncogene which contains point mutation at the 12th codon. In order to determine how theactivated c-Ha-ras oncogene expression governs the cell’s transformation, two pendadecadeoxy-nucleotides AS-1 and AS-2 were synthesized. AS-1 was complementary to the single strandof the first three codons and the upstream sequence close to the ribosome binding site of c-Ha-ras mRNA. AS-2 was complementary to the 3’ end of the first intron and the 5’ end ofthe second exon of c-Ha-ras unripe RNA enclosed in the nucleus. The oligonucleotides couldblock either the translation of c-Ha-ras mRNA or the splicing of c-Ha-ras unripe RNA, thusinhibiting the expression of the activated c-Ha-ras oncogene and the proliferation of thetransformed cells Rat 3-3. The inhibitory effect increased with a growing concentration ofthe Antisense

关键词： Antisense oligodeoxynucleotides transformants inhibition of cell proliferation oncogene

Effects of expressing calmodulin Antisense RNA on cell proliferation and transformation in human hepatoma cell lines

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 1997年第9期42卷 787-791页

作者： Liu, GZ Hu, YY Liu, HT BEIJING NORMAL UNIV DEPT BIOLBEIJING 100875PEOPLES R CHINA

AS the main intracellular receptor of Ca2+,calmodulin（CaM）,which is extensively distribut-ed in various types of eukaryotic cells,is a multifunctional protein regulating the cell growth,differentiation and transform... 详细信息

关键词： calmodulin Antisense RNA cell proliferation and transformation gene expression.