Isolating the cDNA fragment inhibiting nontargeted mutagenesis in vero cell by antisense technology

作     者：Wenwei Hu Yingnian Yu Xingruo Chen Tao Song Haiyang Xie 

作者机构：Zhejiang Med Univ Dept Pathophysiol Hangzhou 310031 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1999年第44卷第6期

页      面：533-537页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：National Natural Science Foundation of China,NSFC Natural Science Foundation of Zhejiang Province,ZJNSF: 394191 

主　　题：antisense RNA mutation nontargeted genetic instability. 

摘      要：Among the expressed sequence tags (ESTs) which were isolated from genetically instable monkey kidney vero cells induced by N-methyI-N’-nitro-N-nitrosoguanidine (MNNG), a fragment 9 was isolated. When the expression of fragment 9 was blocked through transcripting antisense RNA, MNNGinduced nontargeted mutation frequency enhanced significantly compared with control ( P 0.05). The result showed that the relevant gene of fragment 9 might participate in maintaining cellular genetic stability and be involved in inhibition of nontargeted mutagenesis.