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Observation of granulations in the basal body of ovarioles and follicular dilatations for the determination of physiological age of anopheles gambiae s.s.

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Journal of Coastal Life Medicine 2015年第7期 526-530页

作者： Rodrigue Anagonou Virgile Gnanguenon Fiacre Agossa Bruno Akinro Armand Akpo Martial Gbegbo Albert salako Martin Akogbéto Center of Entomological research of Cotonou 06 BP 2604 CotonouBenin Faculty of sciences and Techniques University of Abomey CalaviCalaviBenin

Objective:To explore ovariole basal body granulations and follicular dilatations for determining physiological age inanopheles gambiaes.s.(***.s.).Methods:Mosquitoes were collected by using window trap catch and identified *** the first lot ofmosquitoes,they were dissected,and ovary was left in distilled water for reading ovarian tracheoles and the second was cut and transferred to another blade in a physiological liquid for verification of ovariole basal body *** same approach was followed with the second lot of mosquitoes where follicular dilatations were found after classic dilaceration of ovaries were transferred into physiological *** other body parts of mosquitoes were used to identify the species of *** by ***:Among the *** *** the first lot,the method of Detinova determined the age of 89 mosquitoes vers.s.114 for the observation of granulations(P>0.05).Among the *** *** the second lot,the method of Detinova determined the age of 84 mosquitoes vers.s.93 for the observation of follicular dilatations(P>0.05).Unlike the method of Detinova,observation of follicular dilatations and basal body granulations of ovarioles were possible beyond the stage II ***:Overall,the observation of follicular dilatations and ovariole basal body granulations are reliable for the determination of the physiological age ***,these two methods can be used beyond the stage II.

关键词： anopheles gambiae s.s. Physiological age Ovarioles Dilatations Granulations

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Larvicidal efficacy of monoterpenes against the larvae of anopheles gambiae

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 290-294页

作者： Eliningaya J.Kweka Tamires Cardoso Lima Chrian M.Marciale Dami?o Pergentino de sousa Division of Livestock and Human Diseas.s.Vector Control Tropical Pesticides Research Institute Department of Medical Parasitology and Entomology Catholic University of Health and Allied Sciences Department of Pharmacy Federal University of Sergipe Department of Pharmaceutical sciences Federal University of Paraíba

Objective:To evaluate the larvicidal efficacy of eight volatile components of essential oils against 3rd instar larvae of anopheles gambiae ***:Larvicidal effects of each compound were evaluated in both laboratory and semi-field *** solution was prepared and serial dilutions were made in six concentrations for each compound.A total of 20 larvae were exposed to larvicides for each replicate and monitored at intervals of 12,24,48 and 72 *** monitoring was done on bas.s.of dead and live larvae in all ***:All assayed compounds were larvicides and presented varying degrees of larval toxicity,with LC50 values ranging from 1.28 to 1 938.92 mg/L depending on the treatment time(12,24,48 or 72 h).(-)-Perillyl alcohol presented the strongest larvicidal activity towards anopheles gambiae larvae,with LC_(50) values of 73.60,18.36,1.72 and1.28 mg/L after 12,24,48 and 72 h of exposure,*** next strongest were(-)-isopulegol(LC_(50)= 135.10,49.39,34.39 and 20.22 mg/L) and(-)-carvone epoxide(LC_(50)= 168.86,124.74,80.84 and 23.46 mg/L).After 12,24 and 48 h of treatment,hydroxydihydrocarvone was the least toxic compound,with LC_(50) values of 1 938.92,1 172.18 and 401.03 mg/L,***:The data obtained in this.s.udy suggest that all evaluated monoterpenes,especially(-)-perillyl alcohol,have remarkable larvicidal effects and may be considered as potential sources for the development of suitable natural larvicides for mosquito management *** small-scale field trials.s.ould be conducted.

关键词： Larvicidal activity Malaria anopheles gambiae s.s. Essential oils Monoterpenes Natural products Mosquito

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