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Molecular cloning of Japanese eel anguilla japonica TNF-α and characterization of its expression in response to LPS, poly I:C and Aeromonas hydrophila infection

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Chinese Journal of Oceanology and Limnology 2014年第5期32卷 1046-1059页

作者：冯建军关瑞章郭松林林鹏 Frank ZADLOCK College of Fisheries Jimei University Engineering Research Center of Eel Modern Industry Technology Ministry of Education Key Laboratory of Healthy Mariculture for the East China Sea Ministry of Agriculture Department of Biology Seton Hall University

As a potent pleiotropic cytokine, tumor necrosis factor-alpha （TNF-ct） plays an important role in innate immune responses. The cDNA sequence and genomic structure of the TNF-α gene （AjTNF-c0 in the Japanese eel （anguilla japonica） were identified and characterized. The full-length AjTNF-α cDNA was 1 546 bp, including a 5＇-untranslated region （UTR） of 13 bp, a 3＇-UTR of 879 bp and an open reading frame of 654 bp encoding a protein of 218 amino acids. The full-length genomic sequence of AjTNF-ct was 2 392 bp and included four exons and three introns. The putative AjTNF-α protein contained TNF family signature motifs, including a protease cleavage site, a transmembrane domain and two conserved cysteine residues. Quantitative real-time reverse transcription PCR analysis revealed AjTNF-α expression in a wide range of tissues, with predominant expression in blood and liver. Lower levels of expression were seen in spleen, gills, kidney, intestine, heart, and skin, with very low levels in muscle. The modulation of AjTNF-ct expression after injection of eels with lipopolysaccharide （LPS）, the viral mimic, poly I：C, or Aeromonas hydrophila was assessed in blood, liver, and kidney. In blood, TNF-α mRNA levels increased rapidly and then rapidly decreased after stimulation with LPS, poly I：C or A. hydrophila. However, the response to LPS and A. hydrophila peaked at 6 h while for poly I：C the peak was at 12 h. In liver, after injection with A. hydrophila, an up- and down-regulation of AjTNF-ct expression occurred twice, peaking at 6 h and 24 h, respectively. No remarkable increase of AjTNF-α expression appeared in liver until 72 h after LPS or poly I：C treatment. In kidney, ApiNF-α expression increased significantly only at 72 h post-stimulation with LPS orA. hydrophila. Our results suggest that AjTNF-α plays an important role in fish in the defense against viral and bacterial infection.

关键词： tumor necrosis factor-alpha anguilla japonica cDNA mRNA expression immune response

Gene-associated microsatellite markers confi rm panmixia and indicate a diff erent pattern of spatially varying selection in the endangered Japanese eel anguilla japonica

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Journal of Oceanology and Limnology 2020年第5期38卷 1572-1583页

作者： YU Lei LIU Yanfang LIU Jinxian CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China.

The Japanese eel(anguilla japonica)is a commercially important fi sh species in East Asia and its recruitment has been rapidly declining since *** the genetic population structure of *** is the basis of multinational cooperation on its management and protection,due to its large distribution ***-associated markers have been proved powerful in delineating fi ne-scale population genetic structure and spatially varying *** the present study,we developed 24 polymorphic gene-associated microsatellite markers including 18 loci associated with the genes under selection in the two North Atlantic eel species(anguilla anguilla and anguilla rostrata)and 6 loci based on transcript sequences.A total of 13 geographic populations were sampled across its distribution range,including 11 samples from China(9 from China’s mainland and 2 from Taiwan region),and 2 samples from Japan.A total of 416 individuals(mostly glass eels)were collected and genotyped at the 24 *** measures of diff erentiation were accordant with a panmictic scenario(F ST=-0.001)in *** footprints of spatially varying selection were found,indicating that the selection pattern in *** might be diff erent from that in the two North Atlantic eel *** suggest that *** should be managed as a single unit and management and conservation eff orts must be coordinated at the international level,as overexploitation in any region will decrease its recruitment across the whole distributional range.

关键词： Japanese eel anguilla japonica panmixia gene-associated microsatellite spatially varying selection

Molecular characterization,expression patterns,and subcellular localization of a classical and a novel nonclassical MHC class Iαmolecules from Japanese eel anguilla japonica

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渔业学报（英文） 2023年第2期8卷 108-123页

作者： Jianjun Feng Xinwei Peng Peng Lin Yilei Wang Ziping Zhang Yuankai Xu Pengyun Chen Jingying Wu Engineer Research Center of Eel Modern Industry Technology Ministry of EducationXiamen361021China College of Fisheries Jimei UniversityXiamen361021China College of Animal Science Fujian Agriculture and Forestry UniversityFuzhou350002China Ningbo Institute of Oceanography Ningbo315832China

Major histocompatibility complex(MHC)molecules play a critical role in the immune response of vertebrate animals by presenting foreign antigens to T *** this study,we first cloned and identified a classical and a novel nonclassical MHC Iαmolecules from Japanese eel(anguilla japonica),named as AjMHC I-UBA and AjMHC I-L,*** full-length cDNA of AjMHC I-UBA contains an open reading frame(ORF)of 1047 bp encoding a predicted protein of 348 amino acids,while AjMHC I-L 1089 bp encodes 362 amino *** multiple alignment of the amino acid sequence showed that AjMHC I-UBA and AjMHC I-L consist of an N-terminal MHC I superfamily domain withinα1 andα2 helices,an IgC-MHC Iα3 domain,and a transmembrane region close to the C-terminal,which are similar to other fish and mammal *** polymorphism analysis showed that eight different major alleles of AjMHC I-UBA,named as AjMHC I-UBA*0101~1001,were identified from six Japanese eel ***,a distinguishing signature of the nonclassical L-lineage genes specific motif“HINMTL”,including an N-glycosylation site(NXS/T),was present in theα3 domain of AjMHC I-L *** the predicted three-dimensional structures of AjMHC I-UBA and AjMHC I-L are similar to that of human MHC Iα,phylogenetic analysis showed that these two protein molecules belong to classical MHC I UBA gene of U-lineage and nonclassical MHC I gene of L-lineage,*** real-time polymerase chain reaction(qRT-PCR)analysis revealed that the highest expression of AjMHC I-UBA and AjMHC I-L was found in the intestine and the expression level of AjMHC I-UBA in all of the examined tissues was significantly higher than that of AjMHC *** expressions of AjMHC I-UBA and AjMHC I-L in liver,kidney and spleen were significantly induced following injection with the viral mimic poly I:C,LPS,and Aeromonas hydrophila *** vitro,the AjMHC I-UBA and AjMHC I-L transcripts of Japanese eel liver cells were si

关键词： anguilla japonica MHC Iα mRNA expression Subcelluar localization

Gonadal development and silvering of the Japanese Eel(anguilla japonica)in the Yangtze River during downstream migration

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Aquaculture and Fisheries 2017年第4期2卷 173-178页

作者： Xiaoling Gong Dehai Wang Baolong Bao Qian Zhang Xinglong Jiang Liping Liu The Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai 201306China Engineering Research Center of Eel Industrial Technology Ministry of EducationJimei UniversityXiamen 361021China

The Yangtze River and its branches are important habitats for the Japanese Eel(anguilla japonica),however,there are few data on gonadal development and silvering indices of eels during the downstream spawning *** study investigates gonadal development in 121 Japanese eels caught downstream from the Jingjiang Reach of the Yangtze River in October *** found that in females the ovaries of most of the out-migrating population developed earlier than the testes in males,that is,the ovaries reached stage 3(F3)or early stage 4(eF4),while the testes were at stage 2(M2)or stage 3(M3).Gonadal development in eels collected in 2012 was earlier than that of samples caught from the same region in 2007e2008 and from the Pearl River Estuary in *** average age,body size and gonadal-somatic index of females were significantly greater than those in *** both females and males,there were no significant differences between the two stages of gonadal development in any of the morphological indices,which indicates that gonadal development of the Japanese Eel is independent of body *** results help us to understand the spawning population of Japanese Eels in the Yangtze River.

关键词： Japanese eel anguilla japonica Spawning migration Gonadal development Morphological indices

Behavioral migration diversity of the Yangtze River Japanese Eel,anguilla japonica,based on otolith Sr/Ca ratios

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Zoological Research 2011年第4期32卷 442-450页

作者：郭弘艺郑怡唐文乔沈皓魏凯谢正丽 Katsumi Tsukamoto 上海海洋大学鱼类研究室上海201306 复旦大学现代物理研究所上海200433 东京大学海洋大气研究所

The Yangtze River estuary is the main production area of anguilla japonica in China,as well as the only existing fishery area for adult *** eels are distributed in the main rivers and many tributaries from the Yangtze River estuary to the upper Jinsha River,which extend to nearly 3 000 ***,their migration behaviors remain relatively *** analyzed the biological characteristics of 153 specimens of silver eels collected from the Jingjiang section of Yangtze River（31o30＇N,120o42＇E） between September and November,2008,and tested the sagittal Sr/Ca ratios of 27 *** the 153 specimens examined,85 were female and 68 were male,which translated to a female-male ratio of 1：*** ages of the female specimens ranged from 3 to 7 a（average 5.52） with an average total length（TL） of（669±80） mm,average body weight（BW） of（555±229）g,average condition factor of 1.77±0.22,and average gonad somatic index（GSI） of 1.32±*** ages of the males ranged from 3 to 5 a（average：4.38） with an average TL of（518±51） mm,average BW of（234±76） g,average condition factor of 1.62±0.18,and average GSI of 0.21±*** biological parameters of females were significantly larger than those of the male specimens（P0.05）.According to the average Sr/Ca ratio（7.99±1.05） ×10-3 of the elver mark of sagitta,17 individuals（62.96%） were river eels and 10 individuals（37.04%） were estuarine *** 16 females,13 individuals（81.25%） were river eels and 3 were estuarine eels,while of 11 males,36.36% were river eels and 63.64% were estuarine *** analysis on Sr/Ca ratios for every growth layer group（GLG） indicated there were no significant differences between second-age males and ***,significant differences were observed between the third-age,fourth-age,and migration-age male and female *** was likely related to the fact that second-age eels of both sexes stay in the same inhabitation waters;however,as they grow older,they move to different areas.

关键词： Sagitta Age composition Growth parameter anguilla japonica Silver eel Yangtze River estuary

鳗鲡寄生伪指环虫(单殖吸虫)的危害性、生物学和治疗试验

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Current Zoology 1984年第2期30卷 173-180+208页

作者：钱宝珍吴宝华杭州大学生物系浙江人民卫生实验院寄生虫病研究所

本文研究了人工放养池内引起鳗鲡鳃病的寄生虫是秉伪指环虫和鳗鲡伪指环虫。影响秉伪指环虫纤毛幼虫孵化的因素,与温度和寄主粘液密切相关,而与光照可能无关。在实验室里,分别用药物处理带有病原体的鳗苗,表明:用0.3—0.5ppm90％,晶体... 详细信息

本文研究了人工放养池内引起鳗鲡鳃病的寄生虫是秉伪指环虫和鳗鲡伪指环虫。影响秉伪指环虫纤毛幼虫孵化的因素,与温度和寄主粘液密切相关,而与光照可能无关。在实验室里,分别用药物处理带有病原体的鳗苗,表明:用0.3—0.5ppm90％,晶体敌百虫,可达全部杀灭病原体的良好效果,1％氯化钠长期药浴也有显著效果。

关键词： Pseudodactylogyrus anguilla japonica Pathogenicity Biology Treatment.

日本鳗鲡巨噬细胞移动抑制因子基因鉴定及表达分析

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日本鳗鲡巨噬细胞移动抑制因子基因鉴定及表达分析