The Metabolism of Methylene Blue and Its Derivatives in Japanese Eel(Anguilla Japonica)

作     者：WANG Yali HUANG Xuanyun DU Bingtan LV Xinmei SUN Jing ZHENG Ruizhou HU Kun WANG Yali;HUANG Xuanyun;DU Bingtan;LV Xinmei;SUN Jing;ZHENG Ruizhou;HU Kun

作者机构：National Pathogen Collection Center for Aquatic AnimalsShanghai 201306China National Demonstration Center for Experimental Fisheries Science EducationShanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of AquacultureShanghai 201306China East China Sea Fisheries Research InstituteChinese Academy of Fishery SciencesShanghai 200090China 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2020年第19卷第3期

页      面：653-658页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 08[工学] 09[农学] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by the National Key R&D Program of China (No.2019YFD0900102) the Science and Technology Commission of Shanghai Municipality (Nos.17050502100 and 18391901500) the National Natural Resources Platform of China Knowledge Service Platform of Shanghai Ocean University 

主　　题：methylene blue Anguilla japonica thiazine dye aquaculture 

摘      要：Methylene blue(MB) is commonly used in aquaculture as a fungicide and antidotes. This study was designed to explore the pharmacokinetics of MB in Japanese eel(Anguilla Japonica) immersed in 10 mg L^-1 and 20 mg L^-1 MB for 0.5 h. The concentrations of MB and its derivatives in the blood, liver, kidney, skin and muscle were determined by HPLC after immersion. The results showed that the changes of drug concentrations in Japanese eel were basically the same in different dose groups, showing a general trend of increasing at first and then decreasing, but the peak time was slightly different. The peak concentration of the drug was positively correlated with the dose level. The peak concentrations of MB in MB(20 mg L^-1) group, MB, azure A and azure B in the tissues of Japanese eel were significantly higher than those in MB(10 mg L^-1) group. Moreover, MB, azure A and azure B remained for a long time and could still be detected at 64 days, and azure C was not detected in tissues.