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anaerobic metabolism and thermal tolerance:The importance of opine pathways on survival of a gastropod after cardiac dysfunction

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Integrative Zoology 2017年第5期12卷 361-370页

作者： Guodong HAN Shu ZHANG Yunwei DONG State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamenChina Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources Xiamen UniversityXiamenChina

Organisms on rocky shores are frequently exposed to high temperatures,which cause impairment of cardiac function and retard cellular oxygen ***,some gastropods can survive at several degrees Celsius higher than their Arrhenius break temperature of cardiac function(ABT),indicating the importance of anaerobic metabolism for their thermal *** measured the global molecular responses to heat stress in limpet Cellana toreuma using 454 GS-FLX to investigate the variations of genes involved in anaerobic metabolism at high ***,the gene expression levels of 4 anaerobic enzymes and activity of alanopine dehydrogenase(AlDH),which is involved in opine pathway,were measured in response to elevated temperature.A total of 19 heat shock proteins(HSPs)were determined using real-time PCR at different *** high temperatures,the extensive upregulation of HSP genes was an effective but energetically expensive form of protection to prevent thermal *** upregulation of hypoxia-inducible factor 1 alpha mRNA indicated the condition of cellular hypoxia and the high gene expression and enzyme activity of AlDH suggested that opine pathway was the main anaerobic *** results implied that anaerobic metabolism was enhanced to provide energy in the face of thermal *** findings highlight the ecological significance of the anaerobic metabolism of gastropods to thermal *** predicting the ecological impact of global warming on the distribution of gastropods,the role of anaerobic pathways should be evaluated.

关键词： anaerobic metabolism cardiac function enzyme activity thermal stress transcriptome

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Thermogenic protein UCP1 and UCP3 expression in non- small cell lung cancer： relation with glycolysis and anaerobic metabolism

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Cancer Biology & Medicine 2017年第4期14卷 396-404页

作者： Alexandra Giatromanolaki Konstantina Balaska Dimitra Kalamida Christos Kakouratos Efthimios Sivridis Michael I. Koukourakis Department of Pathology Department of Radiotherapy/Oncology Democritus University of Thrace University Hospital ofAlexandroupolis Alexandroupolis 68100 Greece

Uncoupling protein 1(UCP1)is a proton transporter/channel residing on the inner mitochondrial membrane and is involved in cellular heat *** immunohistochemistry,we investigated the expression of UCP1 and UCP3 in a series of 98 patients with non-small cell lung cancer(NSCLC)treated with *** patterns were correlated with histopathological variables,prognosis,and the expression of enzymes/proteins related to cell *** epithelium did not express UCP1 or UCP3,while alveolar cells strongly expressed *** tumors,strong expression of UCP1 and UCP3 was recorded in43/98(43.8%)and 27/98(27.6%)cases,***1 was significantly associated with squamous cell histology(P=0.05),whilst UCP3 was more frequently overexpressed in large cell carcinomas(P=0.08),and was inversely related to necrosis(P=0.009).In linear regression analysis,UCP1 was directly related to markers of glycolysis[hexokinase(HXKII)and phosphofructokinase(PFK1)]and anaerobic glucose metabolism[pyruvate dehydrogenase kinase(PDK1)and lactate dehydrogenase(LDH5)].UCP3 was directly linked with a glucose transporter(GLUT2),monocarboxylate transporter(MCT2),glycolysis markers(PFK1 and aldolase),and with the phosphorylation of pyruvate dehydrogenase(p PDH).Kaplan-Meier survival analysis showed that UCP3 was significantly related to poor prognosis in squamous cell carcinomas(P=0.04).UCP1 and UCP3 are overexpressed in a large subgroup of non-small cell lung tumors and their expression coincides with increased glucose absorption,intensified glycolysis,and anaerobic glucose *** UCPs are targets for therapeutic interventions in lung cancer is a hypothesis that demands further investigation.

关键词： Lung cancer thermogenesis UCP1 UCP3 glycolysis anaerobic metabolism

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Tail display is regulated by anaerobic metabolism in an Asian agamid lizard

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Integrative Zoology 2021年第5期16卷 729-740页

作者： Xinxin ZHU Xia QIU Xiaolong TANG Yin QI Chengdu Institute of Biology Chinese academy of sciencesChengduChina University of Chinese Academy of Sciences BeijingChina Institute of Biochemistry and Molecular Biology School of Life SciencesLanzhou UniversityLanzhouChina

Understanding the mechanism underlying signal variation is an important goal in the study of animal *** potential causes have been proposed for signal variation,including environmental noise(***,sound),energy limitation,and predation risk,among others,but the physiological control of many signals are often ***,we examined the correlation between tail display signal variation and energy metabolic activity using an Asian agamid lizard Phrynocephalus *** tail display signals were observed in the field,and blood lactate concentration as well as 2 energy metabolic enzymes was *** results showed that average tail coil speed was positively associated with blood lactate concentration,while tail coil duration was negatively associated with LDH *** also found that average tail lash speed was positively associated with blood lactate concentration,suggesting that the tail display behavior of *** was regulated by anaerobic ***,the correlation between tail display behavior and energy metabolism was not *** together,our research provides insight into the physiological mechanisms underlying tail display variation in lizards,and suggests that tail display variation likely transmits important information on individual body condition and resource holding potential.

关键词： anaerobic metabolism lizard Phrynocephalus vlangalii signal variation tail display

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Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli

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Synthetic and Systems Biotechnology 2023年第4期8卷 629-639页

作者： Karel Olavarria Marco V.Becker Diana Z.Sousa Mark C.M.van Loosdrecht S.Aljoscha Wahl Laboratory of Microbiology Wageningen University and ResearchStippenenweg 46708 WEWageningenThe Netherlands Centre for Living Technologies Eindhoven-Wageningen-Utrecht AlliancePrincetonlaan 63584 CBUtrechtThe Netherlands Department of Biotechnology Applied Sciences FacultyDelft University of Technologyvan der Maasweg 92629 HZDelftThe Netherlands Lehrstuhl für Bioverfahrenstechnik Friedrich-Alexander-Universit¨atPaul-Gordan-Strasse 391052ErlangenGermany

Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages,such as increased yields and reduced energy ***,lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic *** stoichiometric analyses and genetic modifications are frequently employed in metabolic engineering,the use of thermodynamic tools to evaluate the feasibility of planned interventions is less *** this study,we propose a novel metabolic engineering strategy to achieve an efficient anaerobic production of poly-(R)-3-hydroxybutyrate(PHB)in the model organism Escherichia *** approach involves re-routing of two-thirds of the glycolytic flux through non-oxidative glycolysis and coupling PHB synthesis with NADH *** complemented our stoichiometric analysis with various thermodynamic approaches to assess the feasibility and the bottlenecks in the proposed engineered *** to our calculations,the main thermodynamic bottleneck are the reactions catalyzed by the acetoacetyl-CoAβ-ketothiolase(EC 2.***.1.9)and the acetoacetyl-CoA reductase(EC 1.***.1.36).Furthermore,we calculated thermodynamically consistent sets of kinetic parameters to determine the enzyme amounts required for sustaining the conversion *** the case of the engineered conversion route,the protein pool necessary to sustain the desired fluxes could account for 20%of the whole cell dry weight.

关键词： Pathway feasibility analysis Protein cost anaerobic metabolism Engineered pathways Metabolite concentrations

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Function of Lactate Dehydrogenase in Cardiac and Skeletal Muscle of Phrynocephalus Lizard in Relation to High-Altitude Adaptation

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Asian Herpetological Research 2018年第4期9卷 258-274页

作者： Huihui WANG Xiaolong TANG Yan WANG Yuxia FENG Peng PU Shengkang MEN Youli ZHAO Zhennan PENG Qiang CHEN Institute of Biochemistry and Molecular Biology School of Life ScienceLanzhou UniversityLanzhou 730000China Lanzhou University Second Hospital Lanzhou 730000China School of Life Science Lanzhou UniversityLanzhou 730000China

Poikilothermic animals living in high-altitude environments can be greatly affected by the anaerobic metabolism and lactate recycling, which are catalyzed by an enzyme called lactate dehydrogenase(LDH). However, the function and possible regulatory mechanisms of their anaerobic glycolysis remained elusive. We compared the difference in LDH between a native high-altitude(4 353 m) lizard, Phrynocephalus erythrurus, and a closely related species, Phrynocephalus przewalskii that lives in intermediate altitude environment(1 400 m). The activity of LDH, the concentration of lactate, the distribution of isoenzyme, and the mRNA amounts of Ldh-A and Ldh-B were determined. In cardiac muscle, the lactate-forming activity of P. erythrurus in LDH was higher than of P. przewalskii LDH at all three temperatures tested(10 °C, 25 °C and 35 °C), while lactate-oxidation activity of LDH was significantly different between the two species only at 25 °C and 35 °C. In skeletal muscle, both lactate-forming and lactate-oxidation rates of P. erythrurus were lower than that of P. przewalskii. There was a higher proportion of H subunit and a significantly higher expression of Ldh-B, with a concomitant decrease of lactate concentration in P. erythrurus. These results indicate that P. erythrurus may have a strong potential for anaerobic metabolism, which is likely adapted to the hypoxic environment at high altitudes. Furthermore, P. erythrurus is capable of oxidizing more lactate than P. przewalskii. The Ldh-A cDNA of the two species consists of a 999 bp open reading frame(ORF), which encodes 332 amino acids, while Ldh-B cDNA consists of a 1 002 bp ORF encoding 333 amino acids. LDHA has the same amino acid sequence between the two species, but three amino acid substitutions(V12 I, N21S and N318K) were observed in LDHB. Structure analysis of LDH indicated that the substitutions of residues Val12 and Asp21 in P. erythrurus could be responsible for the highaltitude adaptation. The LDH characteristi

关键词： lactate dehydrogenase(LDH) high altitude adaptation anaerobic metabolism Phrynocephalus erythrurus

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The tortuous path of lactate shuttle discovery:From cinders and boards to the lab and ICU

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Journal of Sport and Health Science 2020年第5期9卷 446-460页

作者： George A.Brooks Exercise Physiology Laboratory Department of Integrative BiologyUniversity of California BerkeleyCA 94720-3140USA

Once thought to be a waste product of oxygen limited(anaerobic)metabolism,lactate is now known to form continuously under fully oxygenated(aerobic)*** shuttling between producer(driver)and consumer cells fulfills at least 3 purposes;lactate is:(1)a major energy source,(2)the major gluconeogenic precursor,and(3)a signaling *** Lactate Shuttle theory is applicable to diverse fields such as sports nutrition and hydration,resuscitation from acidosis and Dengue,treatment of traumatic brain injury,maintenance of glycemia,reduction of inflammation,cardiac support in heart failure and following a myocardial infarction,and to improve ***,dysregulated lactate shuttling disrupts metabolic flexibility,and worse,supports *** production in cancer(the Warburg effect)is involved in all main sequela for carcinogenesis:angiogenesis,immune escape,cell migration,metastasis,and self-sufficient *** history of the tortuous path of discovery in lactate metabolism and shuttling was discussed in the 2019 American College of Sports Medicine Joseph *** Lecture in Orlando,FL.

关键词： anaerobic metabolism Exercise Glycolysis Oxidative metabolism Warburg Effect

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Group-specific functional patterns of mitochondrion-related organelles shed light on their multiple transitions from mitochondria in ciliated protists

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Marine Life Science & Technology 2022年第4期4卷 609-623页

作者： Zhicheng Chen Jia Li Dayana E.Salas-Leiva Miaoying Chen Shilong Chen Senru Li Yanyan Wu Zhenzhen Yi Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring Guangdong Provincial Key Laboratory for Healthy and Safe AquacultureSchool of Life ScienceSouth China Normal UniversityGuangzhou510631China Department of Biochemistry University of CambridgeCambridgeUK

Adaptations of ciliates to hypoxic environments have arisen independently several *** on mitochondrion-related organelle(MRO)metabolisms from distinct anaerobic ciliate groups provide evidence for understanding the transitions from mitochondria to MROs within *** deepen our knowledge about the evolutionary patterns of ciliate anaerobiosis,mass-culture and single-cell transcriptomes of two anaerobic species,Metopus laminarius(class Armophorea)and Plagiopyla ***(class Plagiopylea),were sequenced and their MRO metabolic maps were *** addition,we carried out comparisons using publicly available predicted MRO proteomes from other ciliate classes(i.e.,Armophorea,Litostomatea,Muranotrichea,Oligohymenophorea,Parablepharismea and Plagiopylea).We found that single-cell transcriptomes were similarly comparable to their mass-culture counterparts in predicting MRO metabolic pathways of *** patterns of the components of the MRO metabolic pathways might be divergent among anaerobic ciliates,even among closely related ***,our findings indicate the existence of group-specific functional relics of electron transport chains(ETCs).Detailed group-specific ETC functional patterns are as follows:full oxidative phosphorylation in Oligohymenophorea and Muranotrichea;only electron-transfer machinery in Armophorea;either of these functional types in Parablepharismea;and ETC functional absence in Litostomatea and *** findings suggest that adaptation of ciliates to anaerobic conditions is group-specific and has occurred multiple *** results also show the potential and the limitations of detecting ciliate MRO proteins using single-cell transcriptomes and improve the understanding of the multiple transitions from mitochondria to MROs within ciliates.

关键词： anaerobic metabolism Mitochondrion-related organelle Single-cell transcriptome Evolutionary transition

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