Group-specific functional patterns of mitochondrion-related organelles shed light on their multiple transitions from mitochondria in ciliated protists

作     者：Zhicheng Chen Jia Li Dayana E.Salas-Leiva Miaoying Chen Shilong Chen Senru Li Yanyan Wu Zhenzhen Yi 

作者机构：Guangzhou Key Laboratory of Subtropical Biodiversity and BiomonitoringGuangdong Provincial Key Laboratory for Healthy and Safe AquacultureSchool of Life ScienceSouth China Normal UniversityGuangzhou510631China Department of BiochemistryUniversity of CambridgeCambridgeUK 

出 版 物：《Marine Life Science & Technology》 (海洋生命科学与技术（英文）)

年 卷 期：2022年第4卷第4期

页      面：609-623页

核心收录：

学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学] 

基　　金：supported by the National Natural Science Foundation of China(Grant Number 32070406) Guangdong Basic and Applied Basic Research Foundation(Grant Number 2022A1515010773) the Science and Technology Planning Project of Guangzhou(Grant Number 202102080168) 

主　　题：Anaerobic metabolism Mitochondrion-related organelle Single-cell transcriptome Evolutionary transition 

摘      要：Adaptations of ciliates to hypoxic environments have arisen independently several *** on mitochondrion-related organelle(MRO)metabolisms from distinct anaerobic ciliate groups provide evidence for understanding the transitions from mitochondria to MROs within *** deepen our knowledge about the evolutionary patterns of ciliate anaerobiosis,mass-culture and single-cell transcriptomes of two anaerobic species,Metopus laminarius(class Armophorea)and Plagiopyla ***(class Plagiopylea),were sequenced and their MRO metabolic maps were *** addition,we carried out comparisons using publicly available predicted MRO proteomes from other ciliate classes(i.e.,Armophorea,Litostomatea,Muranotrichea,Oligohymenophorea,Parablepharismea and Plagiopylea).We found that single-cell transcriptomes were similarly comparable to their mass-culture counterparts in predicting MRO metabolic pathways of *** patterns of the components of the MRO metabolic pathways might be divergent among anaerobic ciliates,even among closely related ***,our findings indicate the existence of group-specific functional relics of electron transport chains(ETCs).Detailed group-specific ETC functional patterns are as follows:full oxidative phosphorylation in Oligohymenophorea and Muranotrichea;only electron-transfer machinery in Armophorea;either of these functional types in Parablepharismea;and ETC functional absence in Litostomatea and *** findings suggest that adaptation of ciliates to anaerobic conditions is group-specific and has occurred multiple *** results also show the potential and the limitations of detecting ciliate MRO proteins using single-cell transcriptomes and improve the understanding of the multiple transitions from mitochondria to MROs within ciliates.