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Journal of Forestry Research 2019年第5期30卷 1735-1741页

作者： Jingyao Tian Siddique Muhammad Ai Chen Peng Chen Jinghong Wang Chengwu Yang Hui Yuan Zhensheng Wang College of Landscape Architecture Northeast Forestry University

In this paper,the ultrasonic-assisted extraction process of flavonoid compounds from leaves of amorpha fruticosa is optimized.In single factor experiments,solid/liquid ratios,ultrasonic power,ethanol concentrations and extraction cycles were experimental factors.Box–Behnken central composite design and RSM analyzed the effects of the four factors on the yield of total flavonoids.The optimal extraction parameters were solid/liquid ratio 1:50 g/mL,ultrasonic power 316 W,ethanol concentration 50%,4 extraction cycles.In the optimized condition,the estimated value of the regression model was 66.6456 mg/g while the measured value was 66.4329 mg/g.

关键词： amorpha fruticosa L. Flavonoid compounds Ultrasonic extraction Response surface methodology

Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and amorpha fruticosa

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Journal of Forestry Research 2019年第2期30卷 483-495页

作者： Xuan Liu Mingguo Jiang Fuqiang Song Heilongjiang University GuangXi University for Nationalities

Arbuscular mycorrhizal fungi(AMF) can colonize and form associations with the roots of amorpha fruticosa L.(desert false indigo). Various genes are induced during the symbiotic process. In this study, de novo transcriptome sequencing using RNA-seq was conducted for the first time for a comprehensive analysis of AMF-A. fruticosa symbionts at the transcript level. We obtained 12 G of raw data from illumina sequencing and recovered 115,786 unigenes with an average length of547 bp, among them 41,848 of significance. A total of2460 diffexpression genes were identified, including 1579 down-regulated and 881 up-regulated genes. A threshold for false discovery rate of \ 0.001 and fold change of [ 1 determined significant differences in gene expression.Using these criteria, we screened 285 significant differentially expressed genes, of which 82 were up-regulated and203 down-regulated. The 82 up-regulated genes were classified according to their functions and assigned into seven categories: stress and defense, metabolism, signaling transduction, protein folding and degradation, energy,protein synthesis, and transcription. The 203 down-regulated genes were screened according to fold change [ 2,and 50 highly significant down-regulated genes were obtained related to stress and defense. The results of this study will provide a useful foundation for further investigation on the metabolic characteristics and molecular mechanisms of AMF associations with leguminous woody shrubs.

关键词： amorpha fruticose Arbuscular mycorrhizal fungi RNA-seq Symbiosis-related gene Quantitative real-time PCR

Karyotype analysis and physical mapping of 45S rDNA in eight species of Sophora, Robinia, and amorpha

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Frontiers in Biology 2006年第3期1卷 290-294页

作者： LIU Bo CHEN Chengbin LI Xiulan QI Liwang HAN Suying Department of Biology College of Life SciencesNankai UniversityTianjin 300071China Laboratory of Cell Biology Research Institute of ForestryChinese Academy of ForestryBeijing 100091China

The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species,and two forms of Sophora,two species of Robina,and one species of amorpha.S.japonica L.,S.japonica L.f.oligophylla Franch.,S.japonica L.f.pendula Loud.,and S.xanthantha C.Y.Ma.are all tetraploids with 2n=28.There were four 45S rDNA sites in pericentromeric regions of two pairs of chromosomes in each of them.S.rubriflora Tsoong.is a triploid with 2n=21,and three sites were located in each satellite of group 5 chromosomes.In R.pseudoacacia L.(2n=2x=22),we examined four intensive signals in te-lomeric regions of two pairs of satellite chromosomes.In R.hispida L.(2n=2x=30),there were four other signals in centromeric regions besides those like in R.pseudoacacia.amorpha fruticosa L.has most chromosomes(2n=40)among the eight materials,however,there were only six 45S rDNA loci and they laid in centromeric regions,and satellites of three pairs of chromosomes.45S rDNA is a valuable chromosomal landmark in karyotype analysis.The distribution and genomic organization of rDNA in the three genera were also discussed.

关键词： Sophora Robinia amorpha karyotype analy-sis 45S rDNA fluorescence in situ hybridization(FISH)

Arbuscular mycorrhizal fungi ameliorate the chemical properties and enzyme activities of rhizosphere soil in reclaimed mining subsidence in northwestern China

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Journal of Arid Land 2019年第1期11卷 135-147页

作者： QIU Lang BI Yinli JIANG Bin WANG Zhigang ZHANG Yanxu Yryszhan ZHAKYPBEK State Key Laboratory of Coal Resources and Safe Mining China University of Mining and Technology (Beijing) College of Geoscience and Surveying Engineering China University of Mining and Technology (Beijing) Satbayev University

In semi-arid region of northwestern China, underground mining subsidence often results in decreased vegetation coverage, impoverishment of soil fertility and water stress. In addition, the physical-chemical and biological properties of soil also change, resulting in more susceptible to degradation. In particular, subsidence causes disturbance of the symbioses of plant and microbe that can play a beneficial role in the establishment of vegetation communities in degraded ecosystems. The objective of this study was to evaluate the effects of revegetation with exotic arbuscular mycorrhizal fungi(AMF) inoculum on the chemical and biological properties of soil over time in mining subsidence areas. Soils were sampled at a depth up to 30 cm in the adjacent rhizosphere of amorpha fruticose Linn. from five reclaimed vegetation communities in northwestern China. In August 2015, a field trial was set up with five historical revegetation experiments established in 2008(7-year), 2011(4-year), 2012(3-year), 2013(2-year) and 2014(1-year), respectively. Each reclamation experiment included two treatments, i.e., revegetation with exotic AMF inoculum(AMF) and non-AMF inoculum(the control). Root mycorrhizal colonization, glomalin-related soil protein(GRSP), soil organic carbon(SOC), soil nutrients, and enzyme activities were also assessed. The results showed that mycorrhizal colonization of inoculated plants increased by 33.3%–163.0% compared to that of non-inoculated plants(P<0.05). Revegetation with exotic AMF inoculum also significantly improved total GRSR(T-GRSP) and easily extracted GRSP(EE-GRSP) concentrations compared to control, besides the T-GRSP in 1-year experiment and the EE-GRSP in 2-year experiment. A significant increase in SOC content was only observed in 7-year AMF reclaimed soils compared to non-AMF reclaimed soils. Soil total N(TN), Olsen phosphorus(P) and available potassium(K) were significantly higher in inoculated soil after 1–7 years of reclamation(except for individual cases), and increased with reclamation

关键词： revegetation mycorrhizal colonization glomalin-related soil proteins arbuscular mycorrhizal fungi coal mining amorpha fruticose

Bio-Morphological Characters of Alien Legume Species, Influencing Their Invasion in Natural Plant Communities

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American Journal of Plant Sciences 2016年第16期7卷 2390-2398页

作者： Yulia Vinogradova Main Botanical Garden Moscow Russia

Five alien legume species, actively invading in natural plant communities in European part of Russia, were studied—Lupinus polyphyllus Lindl., Galega orientalis Lam., Robinia pseudoacacia L., amorpha fruticosa L. and Caragana arborescens L. Distribution ranges (considering their invasive status) were mapped. Not a single bio-morphological character to forecast invasion success in natural plant communities within secondary distribution range was found. The data of key value/importance for explaining invasive success of the species studied were obtained. Two hypotheses— Propagule Pressure and Evolution of Invasiveness—were critically analyzed in view of the received data.

关键词： Alien Species Weeds Legumes Lupinus Galega Robinia amorpha Caragana Invasion Propagule Pressure Hypothesis Evolution of Invasiveness Hypothesis

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栽培基质对紫穗槐幼苗生长的影响

林业科技通讯 2017年第4期 37-39页

作者：刘建军原平市林木种苗管理站山西原平034100

试验选用秸秆+珍珠岩、秸秆+蛭石、珍珠岩+蛭石、珍珠岩、蛭石和秸秆6种栽培基质,研究基质对紫穗槐(amorpha fruticosa Linn.)生长的影响,筛选最适紫穗槐的栽培基质,结果表明,不同基质处理下紫穗槐幼苗的生长不同,即紫穗槐幼苗的生长优... 详细信息

试验选用秸秆+珍珠岩、秸秆+蛭石、珍珠岩+蛭石、珍珠岩、蛭石和秸秆6种栽培基质,研究基质对紫穗槐(amorpha fruticosa Linn.)生长的影响,筛选最适紫穗槐的栽培基质,结果表明,不同基质处理下紫穗槐幼苗的生长不同,即紫穗槐幼苗的生长优劣与育苗基质有关。研究发现秸秆+蛭石为最适合紫穗槐生长的基质,其次是秸秆+珍珠岩,最差基质是单一的珍珠岩。

关键词：紫穗槐 amorpha fruticosa Linn. 栽培基质生长