Transcriptome analysis of the symbiosis-related genes between Funneliformis mosseae and Amorpha fruticosa

作     者：Xuan Liu Mingguo Jiang Fuqiang Song 

作者机构：Heilongjiang University GuangXi University for Nationalities 

出 版 物：《Journal of Forestry Research》 (林业研究（英文版）)

年 卷 期：2019年第30卷第2期

页      面：483-495页

核心收录：

学科分类：09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31070576 and 31270535) the Natural Science Foundation of Heilongjiang Province(No.ZD201206) the Excellent Youth Foundation of Heilongjiang Province(No.JC201306) High-level Talents Support Program of Heilongjiang University(Ecological Restoration Team) 

主　　题：Amorpha fruticose Arbuscular mycorrhizal fungi RNA-seq Symbiosis-related gene Quantitative real-time PCR 

摘      要：Arbuscular mycorrhizal fungi(AMF) can colonize and form associations with the roots of Amorpha fruticosa L.(desert false indigo). Various genes are induced during the symbiotic process. In this study, de novo transcriptome sequencing using RNA-seq was conducted for the first time for a comprehensive analysis of AMF-A. fruticosa symbionts at the transcript level. We obtained 12 G of raw data from illumina sequencing and recovered 115,786 unigenes with an average length of547 bp, among them 41,848 of significance. A total of2460 diffexpression genes were identified, including 1579 down-regulated and 881 up-regulated genes. A threshold for false discovery rate of \ 0.001 and fold change of [ 1 determined significant differences in gene *** these criteria, we screened 285 significant differentially expressed genes, of which 82 were up-regulated and203 down-regulated. The 82 up-regulated genes were classified according to their functions and assigned into seven categories: stress and defense, metabolism, signaling transduction, protein folding and degradation, energy,protein synthesis, and transcription. The 203 down-regulated genes were screened according to fold change [ 2,and 50 highly significant down-regulated genes were obtained related to stress and defense. The results of this study will provide a useful foundation for further investigation on the metabolic characteristics and molecular mechanisms of AMF associations with leguminous woody shrubs.